Oxygen-independent coproporphyrinogen III oxidase - Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)

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Reviewed, UniProtKB/Swiss-Prot P95651 (HEMN_RHOS5)

Last modified November 25, 2008. Version 49. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Oxygen-independent coproporphyrinogen III oxidase
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.99.22

Gene names

Name:	hemN
Ordered Locus Names:	Rsph17025_0977

Organism

Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3) [Complete proteome] [HAMAP]

Taxonomic identifier

349102 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

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Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX By similarity.
Catalytic activity	Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO(2) + 2 L-methionine + 2 5'-deoxyadenosine.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen III (AdoMet route): step 1/1.
Subunit structure	Monomer.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the anaerobic coproporphyrinogen III oxidase family.

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Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW coproporphyrinogen dehydrogenase activity Inferred from electronic annotation. Source: EC coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 452

452

Oxygen-independent coproporphyrinogen III oxidase

PRO_0000109949

Regions

Region

112 – 113

S-adenosyl-L-methionine 2 binding By similarity

Sites

Metal binding

Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Metal binding

Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Metal binding

Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Binding site

S-adenosyl-L-methionine 1 By similarity

Binding site

S-adenosyl-L-methionine 2; via carbonyl oxygen By similarity

Binding site

111

S-adenosyl-L-methionine 1; via amide nitrogen and carbonyl oxygen By similarity

Binding site

144

S-adenosyl-L-methionine 1 By similarity

Binding site

171

S-adenosyl-L-methionine 2 By similarity

Binding site

183

S-adenosyl-L-methionine 2 By similarity

Binding site

208

S-adenosyl-L-methionine 2 By similarity

Experimental info

Sequence conflict

S → C in AAB38508. Ref.1

Sequence conflict

R → P in AAB38508. Ref.1

Sequence conflict

197 – 198

LR → CA in AAB38508. Ref.1

Sequence conflict

302

S → T in AAB38508. Ref.1

Sequence conflict

306

R → G in AAB38508. Ref.1

Sequence conflict

356

G → A in AAB38508. Ref.1

Sequence conflict

402

I → L in AAB38508. Ref.1

Sequence conflict

443

T → S in AAB38508. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P95651-1 [UniParc].

Last modified October 23, 2007. Version 2.
Checksum: 814AFC17EE710CDA
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FASTA

452

49,947

        10         20         30         40         50         60 
MTNIALLQSL GLFDARVPRY TSYPAAPVFS GAVGADFQAQ AIEALDPAVP ISVYIHVPFC 

        70         80         90        100        110        120 
ERLCWFCACR TQGTQTLAPV EAYVGTLLQE LELVKKHLPA GVKAGRLHWG GGTPTILSPE 

       130        140        150        160        170        180 
LIHKLAQAIK AVIPFAEDYE FSVEIDPMMV DEPKIRALSE EGMNRASIGI QDFTDIVQSA 

       190        200        210        220        230        240 
IGREQPFENT RACVETLRRY GVHSLNTDLV YGLPHQNRES LAATIDKVLQ LGPDRVAIFG 

       250        260        270        280        290        300 
YAHVPWMAKR QKLIDENVLP NDMERHELAN LAAKMFTEGG FERIGIDHFA RPDDSMAVAA 

       310        320        330        340        350        360 
RSGKLRRNFQ GYTDDTCPTL LGIGASSISK FEQGYLQNTA ATAAYIKAIE EGRLPGYRGH 

       370        380        390        400        410        420 
RMTDEDYLHG RAIEMIMCEF RLDLPALRAR FGEAAETMVP RITEAAAKFA PFITVDEAGS 

       430        440        450 
MSIEAEGRAL TRMIARVFDA YETPEARYSQ AS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Shi J., Bartnikas T.B., Shapleigh J.P. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome of Rhodobacter sphaeroides ATCC 17025." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. Kaplan S. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases
	U80081 Genomic DNA. Translation: AAB38508.1. CP000661 Genomic DNA. Translation: ABP69878.1.
PIR	T10882.
RefSeq	YP_001167183.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5083193.
GenomeReviews	Gene locus Rsph17025_0977 in contig CP000661_GR.
KEGG	rsq:Rsph17025_0977.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR006638. Elp3/MiaB/NifB. IPR004558. HemN. IPR010723. HemN_C. IPR007197. Radical_SAM. [Graphical view]
Pfam	PF06969. HemN_C. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00538. hemN. 1 hit.
ProtoNet	Search...

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Entry information

Entry name

HEMN_RHOS5

Accession

Primary (citable) accession number: P95651
Secondary accession number(s): A4WR64

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	October 23, 2007
Last modified:	November 25, 2008
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Family and domain databases

Entry information

Relevant documents