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P95651

- HEMN_RHOS5

UniProt

P95651 - HEMN_RHOS5

Protein

Oxygen-independent coproporphyrinogen-III oxidase

Gene

hemN

Organism
Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (23 Oct 2007)
      Previous versions | rss
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    Functioni

    Involved in the heme and chlorophyll biosynthesis. Catalyzes the anaerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX By similarity.By similarity

    Catalytic activityi

    Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO2 + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541S-adenosyl-L-methionine 1By similarity
    Metal bindingi60 – 601Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi64 – 641Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Binding sitei66 – 661S-adenosyl-L-methionine 2; via carbonyl oxygenBy similarity
    Metal bindingi67 – 671Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Binding sitei111 – 1111S-adenosyl-L-methionine 1; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei144 – 1441S-adenosyl-L-methionine 1By similarity
    Binding sitei171 – 1711S-adenosyl-L-methionine 2By similarity
    Binding sitei183 – 1831S-adenosyl-L-methionine 2By similarity
    Binding sitei208 – 2081S-adenosyl-L-methionine 2By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB
    2. coproporphyrinogen dehydrogenase activity Source: UniProtKB
    3. coproporphyrinogen oxidase activity Source: InterPro
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. porphyrin-containing compound biosynthetic process Source: UniProtKB
    2. protoporphyrinogen IX biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Porphyrin biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciRSPH349102:GHE1-2305-MONOMER.
    UniPathwayiUPA00251; UER00323.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Oxygen-independent coproporphyrinogen-III oxidase (EC:1.3.99.22)
    Short name:
    CPO
    Alternative name(s):
    Coproporphyrinogen III dehydrogenase
    Short name:
    CPDH
    Gene namesi
    Name:hemN
    Ordered Locus Names:Rsph17025_0977
    OrganismiRhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
    Taxonomic identifieri349102 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
    ProteomesiUP000000234: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 452452Oxygen-independent coproporphyrinogen-III oxidasePRO_0000109949Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi349102.Rsph17025_0977.

    Structurei

    3D structure databases

    ProteinModelPortaliP95651.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni112 – 1132S-adenosyl-L-methionine 2 bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0635.
    HOGENOMiHOG000257214.
    KOiK02495.
    OMAiRAIEMIM.
    OrthoDBiEOG683S9D.

    Family and domain databases

    Gene3Di3.80.30.20. 1 hit.
    InterProiIPR004558. Coprogen_oxidase_HemN.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    [Graphical view]
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000167. HemN. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00538. hemN. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P95651-1 [UniParc]FASTAAdd to Basket

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    MTNIALLQSL GLFDARVPRY TSYPAAPVFS GAVGADFQAQ AIEALDPAVP    50
    ISVYIHVPFC ERLCWFCACR TQGTQTLAPV EAYVGTLLQE LELVKKHLPA 100
    GVKAGRLHWG GGTPTILSPE LIHKLAQAIK AVIPFAEDYE FSVEIDPMMV 150
    DEPKIRALSE EGMNRASIGI QDFTDIVQSA IGREQPFENT RACVETLRRY 200
    GVHSLNTDLV YGLPHQNRES LAATIDKVLQ LGPDRVAIFG YAHVPWMAKR 250
    QKLIDENVLP NDMERHELAN LAAKMFTEGG FERIGIDHFA RPDDSMAVAA 300
    RSGKLRRNFQ GYTDDTCPTL LGIGASSISK FEQGYLQNTA ATAAYIKAIE 350
    EGRLPGYRGH RMTDEDYLHG RAIEMIMCEF RLDLPALRAR FGEAAETMVP 400
    RITEAAAKFA PFITVDEAGS MSIEAEGRAL TRMIARVFDA YETPEARYSQ 450
    AS 452
    Length:452
    Mass (Da):49,947
    Last modified:October 23, 2007 - v2
    Checksum:i814AFC17EE710CDA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti22 – 221S → C in AAB38508. 1 PublicationCurated
    Sequence conflicti70 – 701R → P in AAB38508. 1 PublicationCurated
    Sequence conflicti197 – 1982LR → CA in AAB38508. 1 PublicationCurated
    Sequence conflicti302 – 3021S → T in AAB38508. 1 PublicationCurated
    Sequence conflicti306 – 3061R → G in AAB38508. 1 PublicationCurated
    Sequence conflicti356 – 3561G → A in AAB38508. 1 PublicationCurated
    Sequence conflicti402 – 4021I → L in AAB38508. 1 PublicationCurated
    Sequence conflicti443 – 4431T → S in AAB38508. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U80081 Genomic DNA. Translation: AAB38508.1.
    CP000661 Genomic DNA. Translation: ABP69878.1.
    PIRiT10882.
    RefSeqiWP_011908017.1. NC_009428.1.
    YP_001167183.1. NC_009428.1.

    Genome annotation databases

    EnsemblBacteriaiABP69878; ABP69878; Rsph17025_0977.
    GeneIDi5083193.
    KEGGirsq:Rsph17025_0977.
    PATRICi23160133. VBIRhoSph94549_1000.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U80081 Genomic DNA. Translation: AAB38508.1 .
    CP000661 Genomic DNA. Translation: ABP69878.1 .
    PIRi T10882.
    RefSeqi WP_011908017.1. NC_009428.1.
    YP_001167183.1. NC_009428.1.

    3D structure databases

    ProteinModelPortali P95651.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 349102.Rsph17025_0977.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP69878 ; ABP69878 ; Rsph17025_0977 .
    GeneIDi 5083193.
    KEGGi rsq:Rsph17025_0977.
    PATRICi 23160133. VBIRhoSph94549_1000.

    Phylogenomic databases

    eggNOGi COG0635.
    HOGENOMi HOG000257214.
    KOi K02495.
    OMAi RAIEMIM.
    OrthoDBi EOG683S9D.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00323 .
    BioCyci RSPH349102:GHE1-2305-MONOMER.

    Family and domain databases

    Gene3Di 3.80.30.20. 1 hit.
    InterProi IPR004558. Coprogen_oxidase_HemN.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    [Graphical view ]
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000167. HemN. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00538. hemN. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Shi J., Bartnikas T.B., Shapleigh J.P.
      Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 17025 / ATH 2.4.3.

    Entry informationi

    Entry nameiHEMN_RHOS5
    AccessioniPrimary (citable) accession number: P95651
    Secondary accession number(s): A4WR64
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3