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P95651

- HEMN_RHOS5

UniProt

P95651 - HEMN_RHOS5

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Protein

Oxygen-independent coproporphyrinogen-III oxidase

Gene

hemN

Organism
Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the heme and chlorophyll biosynthesis. Catalyzes the anaerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX (By similarity).By similarity

Catalytic activityi

Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO2 + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541S-adenosyl-L-methionine 1By similarity
Metal bindingi60 – 601Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi64 – 641Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Binding sitei66 – 661S-adenosyl-L-methionine 2; via carbonyl oxygenBy similarity
Metal bindingi67 – 671Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Binding sitei111 – 1111S-adenosyl-L-methionine 1; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei144 – 1441S-adenosyl-L-methionine 1By similarity
Binding sitei171 – 1711S-adenosyl-L-methionine 2By similarity
Binding sitei183 – 1831S-adenosyl-L-methionine 2By similarity
Binding sitei208 – 2081S-adenosyl-L-methionine 2By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB
  2. coproporphyrinogen dehydrogenase activity Source: UniProtKB
  3. coproporphyrinogen oxidase activity Source: InterPro
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. porphyrin-containing compound biosynthetic process Source: UniProtKB
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Porphyrin biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRSPH349102:GHE1-2305-MONOMER.
UniPathwayiUPA00251; UER00323.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen-independent coproporphyrinogen-III oxidase (EC:1.3.99.22)
Short name:
CPO
Alternative name(s):
Coproporphyrinogen III dehydrogenase
Short name:
CPDH
Gene namesi
Name:hemN
Ordered Locus Names:Rsph17025_0977
OrganismiRhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Taxonomic identifieri349102 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000000234: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452Oxygen-independent coproporphyrinogen-III oxidasePRO_0000109949Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi349102.Rsph17025_0977.

Structurei

3D structure databases

ProteinModelPortaliP95651.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni112 – 1132S-adenosyl-L-methionine 2 bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0635.
HOGENOMiHOG000257214.
KOiK02495.
OMAiRAIEMIM.
OrthoDBiEOG683S9D.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
InterProiIPR004558. Coprogen_oxidase_HemN.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF000167. HemN. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00538. hemN. 1 hit.

Sequencei

Sequence statusi: Complete.

P95651-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNIALLQSL GLFDARVPRY TSYPAAPVFS GAVGADFQAQ AIEALDPAVP
60 70 80 90 100
ISVYIHVPFC ERLCWFCACR TQGTQTLAPV EAYVGTLLQE LELVKKHLPA
110 120 130 140 150
GVKAGRLHWG GGTPTILSPE LIHKLAQAIK AVIPFAEDYE FSVEIDPMMV
160 170 180 190 200
DEPKIRALSE EGMNRASIGI QDFTDIVQSA IGREQPFENT RACVETLRRY
210 220 230 240 250
GVHSLNTDLV YGLPHQNRES LAATIDKVLQ LGPDRVAIFG YAHVPWMAKR
260 270 280 290 300
QKLIDENVLP NDMERHELAN LAAKMFTEGG FERIGIDHFA RPDDSMAVAA
310 320 330 340 350
RSGKLRRNFQ GYTDDTCPTL LGIGASSISK FEQGYLQNTA ATAAYIKAIE
360 370 380 390 400
EGRLPGYRGH RMTDEDYLHG RAIEMIMCEF RLDLPALRAR FGEAAETMVP
410 420 430 440 450
RITEAAAKFA PFITVDEAGS MSIEAEGRAL TRMIARVFDA YETPEARYSQ

AS
Length:452
Mass (Da):49,947
Last modified:October 23, 2007 - v2
Checksum:i814AFC17EE710CDA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221S → C in AAB38508. 1 PublicationCurated
Sequence conflicti70 – 701R → P in AAB38508. 1 PublicationCurated
Sequence conflicti197 – 1982LR → CA in AAB38508. 1 PublicationCurated
Sequence conflicti302 – 3021S → T in AAB38508. 1 PublicationCurated
Sequence conflicti306 – 3061R → G in AAB38508. 1 PublicationCurated
Sequence conflicti356 – 3561G → A in AAB38508. 1 PublicationCurated
Sequence conflicti402 – 4021I → L in AAB38508. 1 PublicationCurated
Sequence conflicti443 – 4431T → S in AAB38508. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80081 Genomic DNA. Translation: AAB38508.1.
CP000661 Genomic DNA. Translation: ABP69878.1.
PIRiT10882.
RefSeqiWP_011908017.1. NC_009428.1.
YP_001167183.1. NC_009428.1.

Genome annotation databases

EnsemblBacteriaiABP69878; ABP69878; Rsph17025_0977.
GeneIDi5083193.
KEGGirsq:Rsph17025_0977.
PATRICi23160133. VBIRhoSph94549_1000.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80081 Genomic DNA. Translation: AAB38508.1 .
CP000661 Genomic DNA. Translation: ABP69878.1 .
PIRi T10882.
RefSeqi WP_011908017.1. NC_009428.1.
YP_001167183.1. NC_009428.1.

3D structure databases

ProteinModelPortali P95651.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 349102.Rsph17025_0977.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP69878 ; ABP69878 ; Rsph17025_0977 .
GeneIDi 5083193.
KEGGi rsq:Rsph17025_0977.
PATRICi 23160133. VBIRhoSph94549_1000.

Phylogenomic databases

eggNOGi COG0635.
HOGENOMi HOG000257214.
KOi K02495.
OMAi RAIEMIM.
OrthoDBi EOG683S9D.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00323 .
BioCyci RSPH349102:GHE1-2305-MONOMER.

Family and domain databases

Gene3Di 3.80.30.20. 1 hit.
InterProi IPR004558. Coprogen_oxidase_HemN.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
[Graphical view ]
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF000167. HemN. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00538. hemN. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Shi J., Bartnikas T.B., Shapleigh J.P.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17025 / ATH 2.4.3.

Entry informationi

Entry nameiHEMN_RHOS5
AccessioniPrimary (citable) accession number: P95651
Secondary accession number(s): A4WR64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 23, 2007
Last modified: November 26, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3