P95646 (TRPE_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component 1
EC=4.1.3.27

Alternative name(s):
Anthranilate synthase component I

Gene names

Name:	trpE
Ordered Locus Names:	RHOS4_05860
ORF Names:	RSP_2004

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II By similarity.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Belongs to the anthranilate synthase component I family.
Sequence caution	The sequence ABA78154.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Anthranilate synthase component 1		PRO_0000154109
Experimental info
	Sequence conflict	196 – 197	2	DA → EC in CAA70293. Ref.1
	Sequence conflict	380	1	V → L in CAA70293. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P95646 [UniParc]. Last modified January 24, 2006. Version 3. Checksum: 3FD8EF30BF3A9466	FASTA	500	54,909
	10 20 30 40 50 60 MTSFESFERG WKAGQNQIVY ARLTADLDTP VSLMLKLAEA RTDTFMLESV TGGEIRGRYS 70 80 90 100 110 120 VVGMKPDLIW QCHGQDSRIN REARFDRQAF QPLEGHPLET LRALIAESRI EMPADLPPIA 130 140 150 160 170 180 AGLFGYLGYD MIRLVEHLPG INPDPLGLPD AVLMRPSVVA VLDGVKGEVT VVAPAWVSSG 190 200 210 220 230 240 LSARAAYAQA AERVMDALRD LDRAPPAQRD FGEVAQVGEM RSNFTHEGYK AAVEKAKDYI 250 260 270 280 290 300 RAGDIFQVVP SQRWAQDFRL PPFALYRSLR KTNPSPFMFF FNFGGFQVVG ASPEILVRLR 310 320 330 340 350 360 DREVTVRPIA GTRKRGATPE EDRALEADLL SDKKELAEHL MLLDLGRNDV GRVAKIGTVR 370 380 390 400 410 420 PTEKFIIERY SHVMHIVSNV VGEIAEGEDA LSALLAGLPA GTVSGAPKVR AMEIIDELEP 430 440 450 460 470 480 EKRGVYGGGV GYFAANGEMD FCIALRTAVL KDETLYIQSG GGVVYDSDPE AEYQETVNKA 490 500 RALRRAAEDA GLFARRAGNG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Rosanas A., Barbe J., Gibert I. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09072 Genomic DNA. Translation: CAA70293.1. CP000143 Genomic DNA. Translation: ABA78154.1. Different initiation.
RefSeq	YP_352055.1. NC_007493.1.
3D structure databases
ProteinModelPortal	P95646.
ModBase	Search...
Protein-protein interaction databases
STRING	P95646.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3719337.
GenomeReviews	Gene locus RHOS4_05860 in contig CP000143_GR.
KEGG	rsp:RSP_2004.
NMPDR	fig|272943.3.peg.665.
PATRIC	23150992. VBIRhoSph57909_0893.
Phylogenomic databases
eggNOG	COG0147.
HOGENOM	HBG507440.
PhylomeDB	P95646.
ProtClustDB	PRK13573.
Enzyme and pathway databases
BioCyc	RSPH272943:RSP_2004-MONOMER.
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR019999. Anth_synth_I. IPR006805. Anth_synth_I_N. IPR005256. Anth_synth_I_PabB. IPR015890. Chorismate-bd_C. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
KO	K01657.
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view]
PRINTS	PR00095. ANTSNTHASEI.
SUPFAM	SSF56322. TRPE_1_chor_bd. 1 hit.
TIGRFAMs	TIGR00564. TrpE_most. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRPE_RHOS4

Accession

Primary (citable) accession number: P95646
Secondary accession number(s): Q3J4Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program