Reviewed, UniProtKB/Swiss-Prot P95629 (PUTA_RHIME)

Names and origin

Protein names

Recommended name:
    Bifunctional protein putA
Including the following 2 domains:
    1- Recommended name:
            Proline dehydrogenase
              EC=1.5.99.8
        Alternative name(s):
            Proline oxidase
    2- Recommended name:
            Delta-1-pyrroline-5-carboxylate dehydrogenase
                Short name=P5C dehydrogenase
              EC=1.5.1.12

Gene names

Name:

putA

Organism

Rhizobium meliloti (Sinorhizobium meliloti)

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	1224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidizes proline to glutamate for use as a carbon and nitrogen source, and also functions as a transcriptional repressor of its own gene.
Catalytic activity	L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor. (S)-1-pyrroline-5-carboxylate + NAD(P)(+) + 2 H(2)O = L-glutamate + NAD(P)H.
Cofactor	FAD.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2. Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.
Sequence similarities	In the N-terminal section; belongs to the proline dehydrogenase family. In the C-terminal section; belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Proline metabolism Transcription Transcription regulation
Ligand	DNA-binding FAD Flavoprotein NAD
Molecular function	Oxidoreductase Repressor
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro proline catabolic process Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW proline dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1224	1224	Bifunctional protein putA		PRO_0000056527
Regions
	Region	164 – 501	338	Proline dehydrogenase
	Region	576 – 1032	457	Aldehyde dehydrogenase
Sites
	Active site	800	1	By similarity
	Active site	834	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P95629-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: BEC655494FCE98D1	FASTA	1,224	131,191
	10 20 30 40 50 60 MSPNPLQKPA IDAAPAPFAD FAPPVRPQST LRRAITAAYR RPETECLPPL VEAATQSKEI 70 80 90 100 110 120 RDAAASTARK LIEALRGKHS GSGVEGLVQE YSLSSQEGVA LMCLAERPVR IPDTATRDAL 130 140 150 160 170 180 IRDKIADGNW KSHLGRSRSL FVNAATWGLV VTGKLTSTVN DRTLAARVTR LISRCGEPVI 190 200 210 220 230 240 RRGVDMAMRM MGEQFVTGET IEALKRSKEL EEKGFSYSYD MLRERPTAAD AERYYRDYES 250 260 270 280 290 300 AIHATAKPRG RGIYEGPGIS IKLSALHPRY RQAARVMGEL LPRVKALALL AKNYDIGLNI 310 320 330 340 350 360 DAEEADRLEL SLDLLEVLCL DGDLSGWNGM GFVVQAYGKR CPFVLDFIID LARRSGRRIM 370 380 390 400 410 420 VRLVKGAYWD AEIKRAQLDG LADFPVFTRK IHTDVSYMPR TQAACRDRCG VPQFATHNAQ 430 440 450 460 470 480 TLAAIYHMAG KDFHVGKYEF QCLHGMGEPL YEEVVGRGKL DRPCRIYAPV GTHETLLAYL 490 500 510 520 530 540 VRRLLENGAN SSFVHRINDP KVSIDELIAD PVEVVRAMPV VGAKHDRIAL PAVLFGDART 550 560 570 580 590 600 NSAGFDLSNE ETLASLTEAL RESAAMKWTA LPQFATGPAA GETRTVLNPG DHRDVVGSVT 610 620 630 640 650 660 ETRKRTHGAP CACRRRGAGL GGRLAERAAC LDRAAELMQA RMPTLLGLII REAGKSALNA 670 680 690 700 710 720 IAEVREAIDF LRYYAEQTRR TLGPATPLGP IVCISPWNFP LAIFTGQIAA ALVAGNPVLA 730 740 750 760 770 780 KPAEETPLIA AEGVRILREA GIPASALQLL PGDGRVGAAL VAGRDAGVMF TGSTEVARLI 790 800 810 820 830 840 QAQLADRLSP AGRPVPLIAE TGGQNAMIVD SSALAGQVVG DVITSAFDSA GQRCSALRVL 850 860 870 880 890 900 CLQEDVAGPH PDDAEGRAAR HCISAAPIVF SVDVGPVITS EAKDNIEKHI ERMRGLGRKV 910 920 930 940 950 960 EQIGLASETG VGTFVPPTII ELEKLSDLQR EVFGPVLHVI RYRRDDLDRL VDDVNATGYG 970 980 990 1000 1010 1020 LTFGLHTRLD ETIAHVTSRI KAGNLYINRN IIGAVVGVQP FGGRGLSGTG PKAGGPLYLG 1030 1040 1050 1060 1070 1080 RLVTTAPVPP QHSSVHTDPV LLDFAKWLDG KGARAEVEAA RNAGSSSALG LDLELPGPVG 1090 1100 1110 1120 1130 1140 ERNLYTLHAR GRILLVPATE SGLYHQLAAA LATGNSVAID AASGLQASLK NLPQTVGLRV 1150 1160 1170 1180 1190 1200 SWSKDWAADG PFAGALVEGD AERIRAVNKA IAALPGPLLL VQAASSGEIA RNPDAYCLNW 1210 1220 LVEEVSASIN TAAAGGNASL MAIG

References

[1]	"The Rhizobium meliloti putA gene: its role in the establishment of the symbiotic interaction with alfalfa." Jimenez-Zurdo J.I., Garcia-Rodriguez F.M., Toro N. Mol. Microbiol. 23:85-93(1997) [PubMed: 9004223] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GR4.
[2]	"Sinorhizobium meliloti putA gene regulation: a new model within the family rhizobiaceae." Soto M.J., Jimenez-Zurdo J.I., van Dillewijn P., Toro N. J. Bacteriol. 182:1935-1941(2000) [PubMed: 10715000] [Abstract] Cited for: AUTOREGULATORY ROLE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y08500 Genomic DNA. Translation: CAA69727.1.
PIR	T43218.
3D structure databases
HSSP	HSSP built from PDB template 1K87 based on UniProtKB P09546.
ModBase	Search...
Family and domain databases
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR005933. d-1-pyrroline-5-COlate_DHase-3. IPR002872. Proline_DHase. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. PF01619. Pro_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01238. D1pyr5carbox3. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUTA_RHIME

Accession

Primary (citable) accession number: P95629

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1997
Last modified:	November 25, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)