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Protein

RNA polymerase sigma factor RpoD

Gene

sigA

Organism
Rhizobium etli
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.SAAS annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi645 – 664H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotationSAAS annotation, Sigma factorUniRule annotationSAAS annotation
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma factor RpoDUniRule annotation
Alternative name(s):
Sigma-70UniRule annotation
Gene namesi
Name:sigAImported
Synonyms:rpoDUniRule annotation
OrganismiRhizobium etliImported
Taxonomic identifieri29449 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Interacts transiently with the RNA polymerase catalytic core.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliP95597
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini475 – 488RNA_pol_sigma70InterPro annotationAdd BLAST14
Domaini644 – 670RNA_pol_sigma70InterPro annotationAdd BLAST27

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni451 – 521Sigma-70 factor domain-2UniRule annotationAdd BLAST71
Regioni530 – 606Sigma-70 factor domain-3UniRule annotationAdd BLAST77
Regioni619 – 672Sigma-70 factor domain-4UniRule annotationAdd BLAST54

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili402 – 422Sequence analysisAdd BLAST21
Coiled coili437 – 457Sequence analysisAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi475 – 478Interaction with polymerase core subunit RpoCUniRule annotation4

Sequence similaritiesi

Belongs to the sigma-70 factor family. RpoD/SigA subfamily.SAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiENOG4105DG1 Bacteria
COG0568 LUCA

Family and domain databases

Gene3Di1.10.10.102 hits
HAMAPiMF_00963 Sigma70_RpoD_SigA, 1 hit
InterProiView protein in InterPro
IPR014284 RNA_pol_sigma-70_dom
IPR000943 RNA_pol_sigma70
IPR009042 RNA_pol_sigma70_r1_2
IPR007627 RNA_pol_sigma70_r2
IPR007624 RNA_pol_sigma70_r3
IPR007630 RNA_pol_sigma70_r4
IPR007631 RNA_pol_sigma_70_non-ess
IPR007127 RNA_pol_sigma_70_r1_1
IPR013325 RNA_pol_sigma_r2
IPR013324 RNA_pol_sigma_r3/r4-like
IPR012760 RNA_pol_sigma_RpoD_C
IPR028630 Sigma70_RpoD
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF04546 Sigma70_ner, 1 hit
PF03979 Sigma70_r1_1, 1 hit
PF00140 Sigma70_r1_2, 1 hit
PF04542 Sigma70_r2, 1 hit
PF04539 Sigma70_r3, 1 hit
PF04545 Sigma70_r4, 1 hit
PRINTSiPR00046 SIGMA70FCT
SUPFAMiSSF88659 SSF88659, 2 hits
SSF88946 SSF88946, 2 hits
TIGRFAMsiTIGR02393 RpoD_Cterm, 1 hit
TIGR02937 sigma70-ECF, 1 hit
PROSITEiView protein in PROSITE
PS00715 SIGMA70_1, 1 hit
PS00716 SIGMA70_2, 1 hit

Sequencei

Sequence statusi: Complete.

P95597-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATKVKENED AEVERDGATD GPLLDLSDDA VKKMIKAAKK RGYVTMDELN
60 70 80 90 100
AVLPSEEVTS EQIEDTMSML SDMGINVIED EEAEEAGASG GGDDDDAGGD
110 120 130 140 150
EDSEGGELAP SAGTALATAK KKEPTDRTDD PVRMYLREMG SVELLSREGE
160 170 180 190 200
IAIAKRIEAG RETMIAGLCE SPLTFQALII WRDELNEGTT LLREIIDLET
210 220 230 240 250
TYSGPEAKAA PQFQSPEKIE ADRKAAEEKE KTRRARSGDD DITDVGGEGL
260 270 280 290 300
PPEEEEEDED ESNLSLAAME AELRPQVMET LDIIAETYKK LRKLQDQQVE
310 320 330 340 350
QRLAATGTLS SAQERRYKEL KDELIKAVKS LSLNQNRIDA LVEQLYDINK
360 370 380 390 400
RLVQNEGRLL RLAESYAVKR DSFLEQDQGA ELDPNWMKSI GNLAARGWKE
410 420 430 440 450
FAKAENTTIR DIRQEIQNLA TETGISISEF RRIVHMVQKG EREARIAKKE
460 470 480 490 500
MVEANLRLVI SIAKKYTNRG LQFLDLIQEG NIGLMKAVDK FEYRRGYKFS
510 520 530 540 550
TYATWWIRQA ITRSIADQAR TIRIPVHMIE TINKIVRTSR QMLHEIGREP
560 570 580 590 600
TPEELAEKLA MPLEKVRKVL KIAKEPISLE TPVGDEEDSH LGDFIEDKNA
610 620 630 640 650
LLPIDAAIQA NLRETTTRVL ASLTPREERV LRMRFGIGMN TDHTLEEVGQ
660 670 680
QFSVTRERIR QIEAKALRKL KHPSRSRKLR SFLDS
Length:685
Mass (Da):77,181
Last modified:May 1, 1997 - v1
Checksum:i36577060A65D80EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08818 Genomic DNA Translation: CAA70052.1

Similar proteinsi

Entry informationi

Entry nameiP95597_RHIET
AccessioniPrimary (citable) accession number: P95597
Entry historyiIntegrated into UniProtKB/TrEMBL: May 1, 1997
Last sequence update: May 1, 1997
Last modified: April 25, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)