ID   P95566_9PSED            Unreviewed;       459 AA.
AC   P95566;
DT   01-MAY-1997, integrated into UniProtKB/TrEMBL.
DT   01-MAY-1997, sequence version 1.
DT   27-MAR-2024, entry version 91.
DE   SubName: Full=Isopropylbenzene-2,3-dioxygenase {ECO:0000313|EMBL:AAB36666.1};
GN   Name=ipbA1 {ECO:0000313|EMBL:AAB36666.1};
OS   Pseudomonas sp. JR1.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=47160 {ECO:0000313|EMBL:AAB36666.1};
RN   [1] {ECO:0000313|EMBL:AAB36666.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=JR1 {ECO:0000313|EMBL:AAB36666.1};
RX   PubMed=8899984;
RA   Pflugmacher U., Averhoff B., Gottschalk G.;
RT   "Cloning, sequencing, and expression of isopropylbenzene degradation genes
RT   from Pseudomonas sp. strain JR1: identification of isopropylbenzene
RT   dioxygenase that mediates trichloroethene oxidation.";
RL   Appl. Environ. Microbiol. 62:3967-3977(1996).
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase
CC       alpha subunit family. {ECO:0000256|ARBA:ARBA00008751}.
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DR   EMBL; U53507; AAB36666.1; -; Genomic_DNA.
DR   AlphaFoldDB; P95566; -.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd08881; RHO_alpha_C_NDO-like; 1.
DR   CDD; cd03472; Rieske_RO_Alpha_BPDO_like; 1.
DR   Gene3D; 2.102.10.10; Rieske [2Fe-2S] iron-sulphur domain; 1.
DR   InterPro; IPR043266; RHO_NdoB-like_C.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR   InterPro; IPR015881; Ring-hydroxy_dOase_2Fe2S_BS.
DR   InterPro; IPR015879; Ring_hydroxy_dOase_asu_C_dom.
DR   InterPro; IPR001663; Rng_hydr_dOase-A.
DR   PANTHER; PTHR43756:SF1; 3-PHENYLPROPIONATE_CINNAMIC ACID DIOXYGENASE SUBUNIT ALPHA; 1.
DR   PANTHER; PTHR43756; CHOLINE MONOOXYGENASE, CHLOROPLASTIC; 1.
DR   Pfam; PF00355; Rieske; 1.
DR   Pfam; PF00848; Ring_hydroxyl_A; 1.
DR   PRINTS; PR00090; RNGDIOXGNASE.
DR   SUPFAM; SSF55961; Bet v1-like; 1.
DR   SUPFAM; SSF50022; ISP domain; 1.
DR   PROSITE; PS51296; RIESKE; 1.
DR   PROSITE; PS00570; RING_HYDROXYL_ALPHA; 1.
PE   3: Inferred from homology;
KW   2Fe-2S {ECO:0000256|ARBA:ARBA00022714};
KW   Aromatic hydrocarbons catabolism {ECO:0000256|ARBA:ARBA00022797};
KW   Dioxygenase {ECO:0000256|ARBA:ARBA00022964, ECO:0000313|EMBL:AAB36666.1};
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   NAD {ECO:0000256|ARBA:ARBA00023027};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT   DOMAIN          59..157
FT                   /note="Rieske"
FT                   /evidence="ECO:0000259|PROSITE:PS51296"
SQ   SEQUENCE   459 AA;  52117 MW;  A9E7C91A202D5EDF CRC64;
     MSSIINKEVQ EAPLKWVKNW SDEEIKALVD EEKGLLDPRI FSDQDLYEIE LERVFARSWL
     LLGHEGHIPK AGDYLTTYMG EDPVIVVRQK DRSIKVFLNQ CRHRGMRIER SNFGNAKSFT
     CTYHGWAYDT AGNLVNVPYE KEAFVTKKEG DCGFDKADWG PLQARVDTYK GLIFANWDTE
     APDLKTYLSD ATPYMDVMLD RTEAGTAVIT GMQKTVIPCN WKFAAEQFCS DMYHAGTMAH
     LSGVLSSLPP EMDLSQVKLP SSGNQFRAKW GGHGTGWFND DFALLQAIMG PKVVDYWTKG
     PAAERAKERL GKVLPADRMV AQHMTIFPTC SFLPGINTVR TWHPRGPNEI EVWSFIVVDA
     DAPADIKEEY RRKNIFTFNQ GGTYEQDDGE NWVEVQRGLR GYKARSRPLC AQMGAGVPNK
     NNPEFPGKTS YVYSEEAARG FYHHWSRMMS EPSWDTLKS
//