ID   P95564_PSESP            Unreviewed;       447 AA.
AC   P95564;
DT   01-MAY-1997, integrated into UniProtKB/TrEMBL.
DT   01-MAY-1997, sequence version 1.
DT   27-MAR-2024, entry version 90.
DE   SubName: Full=ISPalpha 2NT {ECO:0000313|EMBL:AAB40383.1};
GN   Name=ntdAc {ECO:0000313|EMBL:AAB40383.1};
OS   Pseudomonas sp.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=306 {ECO:0000313|EMBL:AAB40383.1};
RN   [1] {ECO:0000313|EMBL:AAB40383.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=JS42 {ECO:0000313|EMBL:AAB40383.1};
RX   PubMed=8973308; DOI=10.1016/S0378-1119(96)00462-3;
RA   Parales J.V., Kumar A., Parales R.E., Gibson D.T.;
RT   "Cloning and sequencing of the genes encoding 2-nitrotoluene dioxygenase
RT   from Pseudomonas sp. JS42.";
RL   Gene 181:57-61(1996).
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase
CC       alpha subunit family. {ECO:0000256|ARBA:ARBA00008751}.
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DR   EMBL; U49504; AAB40383.1; -; Genomic_DNA.
DR   PIR; JC5352; JC5352.
DR   AlphaFoldDB; P95564; -.
DR   SMR; P95564; -.
DR   BioCyc; MetaCyc:MONOMER-13326; -.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd08881; RHO_alpha_C_NDO-like; 1.
DR   Gene3D; 2.102.10.10; Rieske [2Fe-2S] iron-sulphur domain; 1.
DR   InterPro; IPR043266; RHO_NdoB-like_C.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR   InterPro; IPR015881; Ring-hydroxy_dOase_2Fe2S_BS.
DR   InterPro; IPR015879; Ring_hydroxy_dOase_asu_C_dom.
DR   InterPro; IPR001663; Rng_hydr_dOase-A.
DR   PANTHER; PTHR43756:SF1; 3-PHENYLPROPIONATE_CINNAMIC ACID DIOXYGENASE SUBUNIT ALPHA; 1.
DR   PANTHER; PTHR43756; CHOLINE MONOOXYGENASE, CHLOROPLASTIC; 1.
DR   Pfam; PF00355; Rieske; 1.
DR   Pfam; PF00848; Ring_hydroxyl_A; 1.
DR   PRINTS; PR00090; RNGDIOXGNASE.
DR   SUPFAM; SSF55961; Bet v1-like; 1.
DR   SUPFAM; SSF50022; ISP domain; 1.
DR   PROSITE; PS51296; RIESKE; 1.
DR   PROSITE; PS00570; RING_HYDROXYL_ALPHA; 1.
PE   3: Inferred from homology;
KW   2Fe-2S {ECO:0000256|ARBA:ARBA00022714};
KW   Aromatic hydrocarbons catabolism {ECO:0000256|ARBA:ARBA00022797};
KW   Dioxygenase {ECO:0000256|ARBA:ARBA00022964};
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   NAD {ECO:0000256|ARBA:ARBA00023027};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT   DOMAIN          37..121
FT                   /note="Rieske"
FT                   /evidence="ECO:0000259|PROSITE:PS51296"
SQ   SEQUENCE   447 AA;  49486 MW;  1CB0E223E528E3BD CRC64;
     MSYQNLVSEA GLTQKLLIHG DKELFQHELK TIFARNWLFL THDSLIPSPG DYVTAKMGVD
     EVIVSRQNDG SVRAFLNVCR HRGKTLVHTE AGNAKGFVCG YHGWGYGSNG ELQSVPFEKE
     LYGDAIKKKC LGLKEVPRIE SFHGFIYGCF DAEAPPLIDY LGDAAWYLEP TFKHSGGLEL
     VGPPGKVVVK ANWKPFAENF VGDIYHVGWT HAAALRAGQS VFSSLAGNAK LPPEGAGLQM
     TSKYGSGMGL TWDYYSGNFS ADMVPDLMAF GAAKQEKLAK EIGDVRARIY RSILNGTVFP
     NNSFLTGSAT FKVWNPIDEN TTEVWTYAFV EKDMPEDLKR RLADAAQRSI GPAGFWESDD
     NENMETLSQN AKKYQSSNSD QIASLGFGKD VYGDECYPGV VGKSAIGETS YRGFYRAYQA
     HISSSNWAEF ENASRNWHTE LTKTTDR
//