Reviewed, UniProtKB/Swiss-Prot P95544 (DHE4_PRERU)

Names and origin

Protein names

Recommended name:
    NAD(P)-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.3
Alternative name(s):
    NAD(P)H-dependent glutamate dehydrogenase

Gene names

Name:

gdhA

Organism

Prevotella ruminicola (Bacteroides ruminicola)

Taxonomic identifier

839 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Prevotellaceae › Prevotella

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	NAD(P)-specific glutamate dehydrogenase		PRO_0000182773
Sites
	Active site	124	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P95544-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 32CAD7F278CF06A9	FASTA	444	48,878
	10 20 30 40 50 60 MKATEVIEKL KAKFPGQPEY IQAVSQVLGT IEEEYNKHPE FEKANLIERL CVPDRILQFR 70 80 90 100 110 120 VSWVDDNGNV QTNLGYRVQH NNAIGPYKGG LRFHKSVNAS ILKFLAFEQT FKNSLTTLPM 130 140 150 160 170 180 GGAKGGSDFD PHGKSDMEVM RFCQAFMNEL YRLIGPDEDV PAGDIGVGGR EVGYMFGQYK 190 200 210 220 230 240 KLTHQFQGIL TGKGLEFGGS LIRPEATGYG NVYFLEDMLK TRGESLEGKT VLVSGSGNVA 250 260 270 280 290 300 QYTIEKLLQL GAKPVTCSDS NGYIYDPDGI DAEKLAFIME LKNVKRGRIK EYAEKYGVKY 310 320 330 340 350 360 VENARPWGEK ADIATPCATQ DEINEAEAKT LIANGVFAVS EGANMPTEPA AIKVFQDAKI 370 380 390 400 410 420 LYCPGKASNA GGVATSGLEM SQNSERLSWT REEVDTKLHN IMDEIHANCV KYGTEPDGYI 430 440 NYVKGANVAG FMKVAKAMMA QGIY

References

[1]

"The NAD(P)H-dependent glutamate dehydrogenase activities of Prevotella ruminicola B(1)4 can be attributed to one enzyme (GdhA), and gdhA expression is regulated in response to the nitrogen source available for growth." Wen Z., Morrison M. Appl. Environ. Microbiol. 62:3826-3833(1996) [PubMed: 8837439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B14.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U82240 Genomic DNA. Translation: AAB40142.1.
PIR	T10487.
3D structure databases
SMR	P95544. Positions 3-444.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.3. 97798.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_PRERU

Accession

Primary (citable) accession number: P95544

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	January 19, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)