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Protein

Glutamyl-tRNA reductase

Gene

hemA

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).UniRule annotation

Miscellaneous

During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.UniRule annotation

Catalytic activityi

L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH.UniRule annotation

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamyl-tRNA reductase (hemA)
  2. Glutamate-1-semialdehyde 2,1-aminomutase (hemL)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei50NucleophileUniRule annotation1
Sitei104Important for activityUniRule annotation1
Binding sitei114SubstrateUniRule annotation1
Binding sitei125SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi199 – 204NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPorphyrin biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciPMUL272843:G1FZ8-720-MONOMER
UniPathwayiUPA00251; UER00316

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA reductaseUniRule annotation (EC:1.2.1.70UniRule annotation)
Short name:
GluTRUniRule annotation
Gene namesi
Name:hemAUniRule annotation
Synonyms:gltX1
Ordered Locus Names:PM0684
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
Proteomesi
  • UP000000809 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001140521 – 434Glutamyl-tRNA reductaseAdd BLAST434

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272843.PM0684

Structurei

3D structure databases

ProteinModelPortaliP95525
SMRiP95525
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 52Substrate bindingUniRule annotation4
Regioni119 – 121Substrate bindingUniRule annotation3

Domaini

Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.UniRule annotation

Sequence similaritiesi

Belongs to the glutamyl-tRNA reductase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C7E Bacteria
COG0373 LUCA
HOGENOMiHOG000109650
KOiK02492
OMAiFAFKCAA

Family and domain databases

Gene3Di3.30.460.30, 1 hit
HAMAPiMF_00087 Glu_tRNA_reductase, 1 hit
InterProiView protein in InterPro
IPR000343 4pyrrol_synth_GluRdtase
IPR015896 4pyrrol_synth_GluRdtase_dimer
IPR015895 4pyrrol_synth_GluRdtase_N
IPR018214 GluRdtase_CS
IPR036453 GluRdtase_dimer_dom_sf
IPR036343 GluRdtase_N_sf
IPR036291 NAD(P)-bd_dom_sf
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF00745 GlutR_dimer, 1 hit
PF05201 GlutR_N, 1 hit
PF01488 Shikimate_DH, 1 hit
PIRSFiPIRSF000445 4pyrrol_synth_GluRdtase, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF69075 SSF69075, 1 hit
SSF69742 SSF69742, 1 hit
TIGRFAMsiTIGR01035 hemA, 1 hit
PROSITEiView protein in PROSITE
PS00747 GLUTR, 1 hit

Sequencei

Sequence statusi: Complete.

P95525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTILVIGINH KTASVAIREK VAFSAEKRVE ALAQIQQQAL AESAVILSTC
60 70 80 90 100
NRTEVYFHHK AIPPQEAESW TARCMQWFAE IHQLSLDALA GCLYSQQNQQ
110 120 130 140 150
AVLHLMRVAC GLDSLVLGEP QILGQVKDAY QLSKMYYQGQ NQPLSSEFSR
160 170 180 190 200
LFQKTFSVAK RVRTETNIGG NAVSVAYGAC SLARQIFDSL KTLNVLLVGA
210 220 230 240 250
GETIELTCRH LLRHGVQRIM IANRTFERAQ HLVTKLDGAE NVQVLALTQL
260 270 280 290 300
QEGLNQADIV ISSTGSPTIL ITQDMVKIAQ KARCDLPMLL VDIAVPRDIE
310 320 330 340 350
ESVGELDSIY HYTVDDLQTI IQRNLVEREK ASAQAWVIIQ QECADFFEWL
360 370 380 390 400
KVHQFSNLIR SYRENAEDIR QILLEKALLA LRQGEDSEAV LQALSYKLTN
410 420 430
KLLHSPTQVM NAMVKTGNST GLALFSSTLK SDVE
Length:434
Mass (Da):48,408
Last modified:April 27, 2001 - v2
Checksum:i648A624862C5E164
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71 – 72TA → AT in CAA71452 (Ref. 1) Curated2
Sequence conflicti91G → D in CAA71452 (Ref. 1) Curated1
Sequence conflicti135M → P in CAA71452 (Ref. 1) Curated1
Sequence conflicti171N → S in CAA71452 (Ref. 1) Curated1
Sequence conflicti282A → V in CAA71452 (Ref. 1) Curated1
Sequence conflicti326V → A in CAA71452 (Ref. 1) Curated1
Sequence conflicti344A → V in CAA71452 (Ref. 1) Curated1
Sequence conflicti379L → F in CAA71452 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10430 Genomic DNA Translation: CAA71452.1
AE004439 Genomic DNA Translation: AAK02768.1
RefSeqiWP_010906790.1, NC_002663.1

Genome annotation databases

EnsemblBacteriaiAAK02768; AAK02768; PM0684
KEGGipmu:PM0684
PATRICifig|272843.6.peg.692

Similar proteinsi

Entry informationi

Entry nameiHEM1_PASMU
AccessioniPrimary (citable) accession number: P95525
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 27, 2001
Last modified: March 28, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health