Reviewed, UniProtKB/Swiss-Prot P95524 (DYR6_PROMI)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase type 6
    EC=1.5.1.3
Alternative name(s):
    Dihydrofolate reductase type VI

Gene names

Name:	dhfrVI
Synonyms:	dfrVI

Encoded on

Plasmid pUK672

Organism

Proteus mirabilis

Taxonomic identifier

584 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Proteus

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Subunit structure	Homodimer By similarity.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	Antibiotic resistance Methotrexate resistance One-carbon metabolism Trimethoprim resistance
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW response to methotrexate Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	Dihydrofolate reductase type 6		PRO_0000186423
Regions
	Domain	2 – 156	155	DHFR

Sequences

	Sequence		Length	Mass (Da)
	P95524-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: FE10DDF3724E7D27	FASTA	157	17,444
	10 20 30 40 50 60 MKISLMAAVS ENGVIGSGLD IPWHVQGEQL LFKAMTYNQW LLVGRKTFDS MGKLPNRKYA 70 80 90 100 110 120 VVTRSKIISN DPDVVYFASV ESALAYLNNA TAHIFVSGGG EIYKALIDQA DVIHLSVIHK 130 140 150 HISGDVFFPP VPQGFKQTFE QSFSSNIDYT YQIWAKG

References

[1]	"Nucleotide sequence of dihydrofolate reductase type VI." Wylie B.A., Koornhof H.J. J. Med. Microbiol. 35:214-218(1991) [PubMed: 1941991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: J120.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z86002 Genomic DNA. Translation: CAB06660.1.
3D structure databases
HSSP	HSSP built from PDB template 1DYR based on UniProtKB P16184.
ModBase	Search...
Family and domain databases
InterPro	IPR012259. DHFR. IPR001796. DHFR_reg. [Graphical view]
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR6_PROMI

Accession

Primary (citable) accession number: P95524

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	November 25, 2008
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)