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P95493

- CPG2_PORGI

UniProt

P95493 - CPG2_PORGI

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Protein

Gingipain R2

Gene

rgpB

Organism
Porphyromonas gingivalis (strain ATCC BAA-308 / W83)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Its proteolytic activity is a major factor in both periodontal tissue destruction and in bacterial host defense mechanisms. Activates complement C3 and C5 (By similarity).By similarity

Catalytic activityi

Hydrolysis of proteins and small molecule substrates, with a preference for Arg in P1.

Enzyme regulationi

Inhibited by human histatin-3 1/24 (histatin-5).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei440 – 4401Proton donor
Active sitei473 – 4731Nucleophile

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Virulence

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BioCyciPGIN242619:GHX8-458-MONOMER.
BRENDAi3.4.22.37. 4997.
SABIO-RKP95493.

Protein family/group databases

MEROPSiC25.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Gingipain R2 (EC:3.4.22.37)
Alternative name(s):
Arg-gingipain
Gingipain 2
RGP-2
Gene namesi
Name:rgpB
Synonyms:prtRII, rgp2
Ordered Locus Names:PG_0506
OrganismiPorphyromonas gingivalis (strain ATCC BAA-308 / W83)
Taxonomic identifieri242619 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesPorphyromonadaceaePorphyromonas
ProteomesiUP000000588: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424By similarityAdd
BLAST
Propeptidei25 – 2292051 PublicationPRO_0000026535Add
BLAST
Chaini230 – 736507Gingipain R2PRO_0000026536Add
BLAST

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiP95493.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HTN3P155167EBI-8505881,EBI-738783From a different organism.

Protein-protein interaction databases

IntActiP95493. 1 interaction.
MINTiMINT-8415911.
STRINGi242619.PG0506.

Structurei

Secondary structure

1
736
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi36 – 416
Beta strandi44 – 507
Beta strandi55 – 617
Beta strandi64 – 707
Beta strandi87 – 937
Beta strandi96 – 11217
Helixi130 – 1323
Helixi139 – 1424
Beta strandi151 – 1544
Beta strandi158 – 1603
Beta strandi163 – 1708
Beta strandi173 – 1764
Turni177 – 1804
Beta strandi181 – 19818
Helixi215 – 2217
Beta strandi240 – 2456
Helixi247 – 2526
Helixi254 – 2629
Beta strandi266 – 2716
Helixi272 – 2754
Helixi281 – 29313
Beta strandi299 – 3068
Turni308 – 3103
Beta strandi314 – 3163
Beta strandi319 – 3224
Helixi323 – 3264
Beta strandi329 – 3335
Beta strandi336 – 3427
Helixi347 – 36216
Turni369 – 3724
Beta strandi373 – 3786
Helixi387 – 3893
Helixi392 – 40615
Beta strandi409 – 41810
Helixi421 – 43010
Beta strandi433 – 4397
Beta strandi443 – 4464
Turni447 – 4493
Turni453 – 4553
Helixi456 – 4583
Beta strandi467 – 4748
Beta strandi480 – 4823
Helixi485 – 4917
Beta strandi502 – 5098
Beta strandi512 – 5143
Helixi515 – 52713
Helixi535 – 5373
Helixi539 – 56426
Beta strandi565 – 5695
Beta strandi589 – 5924
Beta strandi596 – 6049
Beta strandi608 – 6136
Beta strandi616 – 6227
Beta strandi627 – 6315
Beta strandi640 – 6478
Beta strandi654 – 6618

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CVRX-ray2.00A230-664[»]
4IEFX-ray2.30A/C/E/G25-229[»]
B/D/F/H230-662[»]
ProteinModelPortaliP95493.
SMRiP95493. Positions 230-661.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP95493.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C25 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG12793.
KOiK08589.
OMAiKENGRMI.
OrthoDBiEOG6BGNW2.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.40.50.10390. 1 hit.
3.40.50.1460. 1 hit.
InterProiIPR029030. Caspase-like_dom.
IPR001769. Gingipain.
IPR029031. Gingipain_N.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR005536. Peptidase_C25_Ig-like_domain.
IPR012600. Propeptide_C25.
IPR026444. Secre_tail.
[Graphical view]
PfamiPF01364. Peptidase_C25. 1 hit.
PF03785. Peptidase_C25_C. 1 hit.
PF08126. Propeptide_C25. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR04183. Por_Secre_tail. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P95493-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKNFSRIVS IVAFSSLLGG MAFAQPAERG RNPQVRLLSA EQSMSKVQFR
60 70 80 90 100
MDNLQFTGVQ TSKGVAQVPT FTEGVNISEK GTPILPILSR SLAVSETRAM
110 120 130 140 150
KVEVVSSKFI EKKDVLIAPS KGVISRAENP DQIPYVYGQS YNEDKFFPGE
160 170 180 190 200
IATLSDPFIL RDVRGQVVNF APLQYNPVTK TLRIYTEIVV AVSETAEAGQ
210 220 230 240 250
NTISLVKNST FTGFEDIYKS VFMNYEATRY TPVEEKENGR MIVIVPKKYE
260 270 280 290 300
EDIEDFVDWK NQRGLRTEVK VAEDIASPVT ANAIQQFVKQ EYEKEGNDLT
310 320 330 340 350
YVLLVGDHKD IPAKITPGIK SDQVYGQIVG NDHYNEVFIG RFSCESKEDL
360 370 380 390 400
KTQIDRTIHY ERNITTEDKW LGQALCIASA EGGPSADNGE SDIQHENIIA
410 420 430 440 450
NLLTQYGYTK IIKCYDPGVT PKNIIDAFNG GISLANYTGH GSETAWGTSH
460 470 480 490 500
FGTTHVKQLT NSNQLPFIFD VACVNGDFLY NVPCFAEALM RAQKDGKPTG
510 520 530 540 550
TVAIIASTIN QSWASPMRGQ DEMNEILCEK HPNNIKRTFG GVTMNGMFAM
560 570 580 590 600
VEKYKKDGEK MLDTWTVFGD PSLLVRTLVP TKMQVTAPAN ISASAQTFEV
610 620 630 640 650
ACDYNGAIAT LSDDGDMVGT AIVKDGKAII KLNESIADET NLTLTVVGYN
660 670 680 690 700
KVTVIKDVKV EGTSIADVAN DKPYTVAVSG KTITVESPAA GLTIFDMNGR
710 720 730
RVATAKNRMV FEAQNGVYAV RIATEGKTYT EKVIVK
Length:736
Mass (Da):80,967
Last modified:October 3, 2003 - v2
Checksum:iC848DD3FAB420833
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581G → D in AAB41892. (PubMed:9705298)Curated
Sequence conflicti246 – 2461P → A in AAB41892. (PubMed:9705298)Curated
Sequence conflicti251 – 2511E → G in AAB41892. (PubMed:9705298)Curated
Sequence conflicti254 – 2541E → K in AAB41892. (PubMed:9705298)Curated
Sequence conflicti398 – 3981I → V in AAB41892. (PubMed:9705298)Curated
Sequence conflicti435 – 4351A → V in AAB41892. (PubMed:9705298)Curated
Sequence conflicti480 – 4823YNV → FSM in AAB41892. (PubMed:9705298)Curated
Sequence conflicti510 – 5101N → D AA sequence (PubMed:9705298)Curated
Sequence conflicti512 – 5121S → Y AA sequence (PubMed:9705298)Curated
Sequence conflicti515 – 5151S → P AA sequence (PubMed:9705298)Curated
Sequence conflicti560 – 5601K → N in AAB41892. (PubMed:9705298)Curated
Sequence conflicti582 – 5821K → E in AAB41892. (PubMed:9705298)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U85038 Genomic DNA. Translation: AAB41892.1.
AF007124 Genomic DNA. Translation: AAC26371.1.
AE015924 Genomic DNA. Translation: AAQ65700.1.
RefSeqiNP_904801.1. NC_002950.2.
WP_010956050.1. NC_002950.2.

Genome annotation databases

EnsemblBacteriaiAAQ65700; AAQ65700; PG_0506.
GeneIDi2552233.
KEGGipgi:PG0506.
PATRICi22978106. VBIPorGin134034_0464.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U85038 Genomic DNA. Translation: AAB41892.1 .
AF007124 Genomic DNA. Translation: AAC26371.1 .
AE015924 Genomic DNA. Translation: AAQ65700.1 .
RefSeqi NP_904801.1. NC_002950.2.
WP_010956050.1. NC_002950.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CVR X-ray 2.00 A 230-664 [» ]
4IEF X-ray 2.30 A/C/E/G 25-229 [» ]
B/D/F/H 230-662 [» ]
ProteinModelPortali P95493.
SMRi P95493. Positions 230-661.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P95493. 1 interaction.
MINTi MINT-8415911.
STRINGi 242619.PG0506.

Protein family/group databases

MEROPSi C25.003.

Proteomic databases

PRIDEi P95493.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAQ65700 ; AAQ65700 ; PG_0506 .
GeneIDi 2552233.
KEGGi pgi:PG0506.
PATRICi 22978106. VBIPorGin134034_0464.

Phylogenomic databases

eggNOGi NOG12793.
KOi K08589.
OMAi KENGRMI.
OrthoDBi EOG6BGNW2.

Enzyme and pathway databases

BioCyci PGIN242619:GHX8-458-MONOMER.
BRENDAi 3.4.22.37. 4997.
SABIO-RK P95493.

Miscellaneous databases

EvolutionaryTracei P95493.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
3.40.50.10390. 1 hit.
3.40.50.1460. 1 hit.
InterProi IPR029030. Caspase-like_dom.
IPR001769. Gingipain.
IPR029031. Gingipain_N.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR005536. Peptidase_C25_Ig-like_domain.
IPR012600. Propeptide_C25.
IPR026444. Secre_tail.
[Graphical view ]
Pfami PF01364. Peptidase_C25. 1 hit.
PF03785. Peptidase_C25_C. 1 hit.
PF08126. Propeptide_C25. 1 hit.
[Graphical view ]
SUPFAMi SSF81296. SSF81296. 1 hit.
TIGRFAMsi TIGR04183. Por_Secre_tail. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative properties of two cysteine proteinases (gingipains R), the products of two related but individual genes of Porphyromonas gingivalis."
    Potempa J., Mikolajczyk-Pawlinska J., Brassell D., Nelson D., Thoegersen I.B., Enghild J.J., Travis J.
    J. Biol. Chem. 273:21648-21657(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 230-651.
    Strain: HG66.
  2. "Characterization of a second cell-associated Arg-specific cysteine proteinase of Porphyromonas gingivalis and identification of an adhesin-binding motif involved in association of the prtR and prtK proteinases and adhesins into large complexes."
    Slakeski N., Bhogal P.S., O'Brien-Simpson N.M., Reynolds E.C.
    Microbiology 144:1583-1592(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 53978 / W50.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-308 / W83.
  4. "Salivary histatin 5 is an inhibitor of both host and bacterial enzymes implicated in periodontal disease."
    Gusman H., Travis J., Helmerhorst E.J., Potempa J., Troxler R.F., Oppenheim F.G.
    Infect. Immun. 69:1402-1408(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  5. "Crystal structure of gingipain R: an Arg-specific bacterial cysteine proteinase with a caspase-like fold."
    Eichinger A., Beisel H.-G., Jacob U., Huber R., Medrano F.-J., Banbula A., Potempa J., Travis J., Bode W.
    EMBO J. 18:5453-5462(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).

Entry informationi

Entry nameiCPG2_PORGI
AccessioniPrimary (citable) accession number: P95493
Secondary accession number(s): O33441
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 3, 2003
Last modified: October 29, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3