Reviewed, UniProtKB/Swiss-Prot P95487 (CCMA_PSEPG)

Names and origin

Protein names

Recommended name:
    Cytochrome c biogenesis ATP-binding export protein ccmA
    EC=3.6.3.41
Alternative name(s):
    Heme exporter protein A

Gene names

Name:	ccmA
Ordered Locus Names:	PputGB1_3896

Organism

Pseudomonas putida (strain GB-1) [Complete proteome] [HAMAP]

Taxonomic identifier

76869 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of the ABC transporter complex ccmAB involved in the biogenesis of c-type cytochromes; once thought to export heme, this seems not to be the case, but its exact role is uncertain. Responsible for energy coupling to the transport system By similarity. HAMAP MF_01707
Catalytic activity	ATP + H2O + heme(In) = ADP + phosphate + heme(Out). HAMAP MF_01707
Subunit structure	The complex is composed of two ATP-binding proteins (ccmA) and two transmembrane proteins (ccmB) Probable. HAMAP MF_01707
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity HAMAP MF_01707.
Sequence similarities	Belongs to the ABC transporter superfamily. CcmA exporter (TC 3.A.1.107) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW heme-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Cytochrome c biogenesis ATP-binding export protein ccmA HAMAP MF_01707		PRO_0000092197
Regions
	Domain	3 – 205	203	ABC transporter
	Nucleotide binding	37 – 44	8	ATP Potential
Experimental info
	Sequence conflict	60 – 69	10	QILLGGKPLA → ADPAGAQRLR in AAC63581. Ref.2
	Sequence conflict	76	1	A → R in AAC63581. Ref.2
	Sequence conflict	86	1	A → P in AAC63581. Ref.2
	Sequence conflict	105	1	H → D in AAC63581. Ref.2
	Sequence conflict	112 – 115	4	AIWA → RL in AAC63581. Ref.2
	Sequence conflict	130 – 132	3	CHT → VPY in AAC63581. Ref.2
	Sequence conflict	150 – 151	2	CP → AR in AAC63581. Ref.2
	Sequence conflict	168 – 174	7	VAQLEAH → AGAAGSS in AAC63581. Ref.2
	Sequence conflict	184	1	T → H in AAC63581. Ref.2
	Sequence conflict	198	1	S → P in AAC63581. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P95487-1 [UniParc]. Last modified April 29, 2008. Version 3. Checksum: F2FA7F68827F0DCB	FASTA	210	22,746
	10 20 30 40 50 60 MTLHLQAAGL ACERDWRLLF EQLDFELGAG DMLQISGPNG SGKTSLLRLL AGLMQPTAGQ 70 80 90 100 110 120 ILLGGKPLAE QRHALASILL WIGHAAGIKD LLTAEENLTW LCALHQPASR EAIWAALEAV 130 140 150 160 170 180 GLRGFEDVPC HTLSAGQQRR VALARLHLAC PPLWILDEPF TALDKQGVAQ LEAHLAAHCE 190 200 210 QGGTVVLTTH HTLERKPSGY RELNLGQWAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of Pseudomonas putida GB-1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The cytochrome c maturation operon is involved in manganese oxidation in Pseudomonas putida GB-1." de Vrind J.P.M., Brouwers G.J., Corstjens P.L.A.M., den Dulk J., de Vrind-de Jong E.W. Appl. Environ. Microbiol. 64:3556-3562(1998) [PubMed: 9758767] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-210.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000926 Genomic DNA. Translation: ABY99786.1. U85716 Genomic DNA. Translation: AAC63581.1.
RefSeq	YP_001670122.1.
3D structure databases
SMR	P95487. Positions 20-195.
ModBase	Search...
Genome annotation databases
GeneID	5871690.
GenomeReviews	Gene locus PputGB1_3896 in contig CP000926_GR.
KEGG	ppg:PputGB1_3896.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG758042.
OMA	FAGLAFE.
Family and domain databases
HAMAP	MF_01707. ABC_ATPase_CcmA. [Tree]
InterPro	IPR003439. ABC_transporter-like. IPR005895. ABC_transptr_haem_export_CcmA. IPR003593. ATPase_AAA+_core. [Graphical view]
PANTHER	PTHR19222:SF5. CcmA. 1 hit.
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01189. ccmA. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. False negative. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51243. CCMA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCMA_PSEPG

Accession

Primary (citable) accession number: P95487
Secondary accession number(s): B0KQ31

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	April 29, 2008
Last modified:	February 9, 2010
This is version 56 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)