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Protein

Carbamate kinase

Gene

cpkA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Carbamate kinase that plays a biosynthetic role in that it produces carbamoyl-phosphate.1 Publication

Catalytic activityi

ATP + NH3 + CO2 = ADP + carbamoyl phosphate.

Temperature dependencei

50% activity is retained after 1 hour at 100 degrees Celsius or 3 hours at 95 degrees Celsius.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.2.2. 5243.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamate kinase (EC:2.7.2.2)
Alternative name(s):
Carbamate kinase-like carbamoylphosphate synthase
Gene namesi
Name:cpkA
Synonyms:cpa
Ordered Locus Names:PF0676
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001851491 – 314Carbamate kinaseAdd BLAST314

Proteomic databases

PRIDEiP95474.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi186497.PF0676.

Structurei

Secondary structure

1314
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Helixi11 – 13Combined sources3
Helixi23 – 42Combined sources20
Beta strandi46 – 50Combined sources5
Helixi54 – 71Combined sources18
Helixi78 – 103Combined sources26
Beta strandi110 – 113Combined sources4
Beta strandi116 – 119Combined sources4
Helixi124 – 126Combined sources3
Beta strandi131 – 137Combined sources7
Helixi139 – 149Combined sources11
Beta strandi152 – 155Combined sources4
Beta strandi161 – 165Combined sources5
Beta strandi170 – 173Combined sources4
Helixi176 – 184Combined sources9
Beta strandi188 – 190Combined sources3
Helixi193 – 195Combined sources3
Beta strandi197 – 202Combined sources6
Beta strandi205 – 208Combined sources4
Helixi215 – 225Combined sources11
Beta strandi229 – 239Combined sources11
Turni243 – 245Combined sources3
Beta strandi254 – 256Combined sources3
Helixi257 – 265Combined sources9
Turni271 – 273Combined sources3
Helixi274 – 287Combined sources14
Beta strandi290 – 296Combined sources7
Helixi297 – 299Combined sources3
Helixi300 – 304Combined sources5
Beta strandi307 – 313Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E19X-ray1.50A/B1-314[»]
ProteinModelPortaliP95474.
SMRiP95474.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP95474.

Family & Domainsi

Sequence similaritiesi

Belongs to the carbamate kinase family.Curated

Phylogenomic databases

eggNOGiarCOG00863. Archaea.
COG0549. LUCA.
HOGENOMiHOG000277403.
KOiK00926.
OMAiADIFMIL.

Family and domain databases

CDDicd04235. AAK_CK. 1 hit.
Gene3Di3.40.1160.10. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR003964. Carb_kinase.
[Graphical view]
PANTHERiPTHR30409. PTHR30409. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000723. Carbamate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00746. arcC. 1 hit.

Sequencei

Sequence statusi: Complete.

P95474-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKRVVIALG GNALQQRGQK GSYEEMMDNV RKTARQIAEI IARGYEVVIT
60 70 80 90 100
HGNGPQVGSL LLHMDAGQAT YGIPAQPMDV AGAMSQGWIG YMIQQALKNE
110 120 130 140 150
LRKRGMEKKV VTIITQTIVD KNDPAFQNPT KPVGPFYDEE TAKRLAREKG
160 170 180 190 200
WIVKEDSGRG WRRVVPSPDP KGHVEAETIK KLVERGVIVI ASGGGGVPVI
210 220 230 240 250
LEDGEIKGVE AVIDKDLAGE KLAEEVNADI FMILTDVNGA ALYYGTEKEQ
260 270 280 290 300
WLREVKVEEL RKYYEEGHFK AGSMGPKVLA AIRFIEWGGE RAIIAHLEKA
310
VEALEGKTGT QVLP
Length:314
Mass (Da):34,429
Last modified:May 1, 1997 - v1
Checksum:iB4496B827581EF42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09829 Genomic DNA. Translation: CAA70972.1.
AB016521 Genomic DNA. Translation: BAA32017.1.
AE009950 Genomic DNA. Translation: AAL80800.1.
PIRiT43855.
RefSeqiWP_011011798.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80800; AAL80800; PF0676.
GeneIDi1468522.
KEGGipfu:PF0676.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09829 Genomic DNA. Translation: CAA70972.1.
AB016521 Genomic DNA. Translation: BAA32017.1.
AE009950 Genomic DNA. Translation: AAL80800.1.
PIRiT43855.
RefSeqiWP_011011798.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E19X-ray1.50A/B1-314[»]
ProteinModelPortaliP95474.
SMRiP95474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0676.

Proteomic databases

PRIDEiP95474.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80800; AAL80800; PF0676.
GeneIDi1468522.
KEGGipfu:PF0676.

Phylogenomic databases

eggNOGiarCOG00863. Archaea.
COG0549. LUCA.
HOGENOMiHOG000277403.
KOiK00926.
OMAiADIFMIL.

Enzyme and pathway databases

BRENDAi2.7.2.2. 5243.

Miscellaneous databases

EvolutionaryTraceiP95474.

Family and domain databases

CDDicd04235. AAK_CK. 1 hit.
Gene3Di3.40.1160.10. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR003964. Carb_kinase.
[Graphical view]
PANTHERiPTHR30409. PTHR30409. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000723. Carbamate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00746. arcC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCPKA_PYRFU
AccessioniPrimary (citable) accession number: P95474
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: May 1, 1997
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.