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P95474

- CPKA_PYRFU

UniProt

P95474 - CPKA_PYRFU

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Protein

Carbamate kinase

Gene

cpkA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Carbamate kinase that plays a biosynthetic role in that it produces carbamoyl-phosphate.1 Publication

Catalytic activityi

ATP + NH3 + CO2 = ADP + carbamoyl phosphate.

Temperature dependencei

50% activity is retained after 1 hour at 100 degrees Celsius or 3 hours at 95 degrees Celsius.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. carbamate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. arginine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamate kinase (EC:2.7.2.2)
Alternative name(s):
Carbamate kinase-like carbamoylphosphate synthase
Gene namesi
Name:cpkA
Synonyms:cpa
Ordered Locus Names:PF0676
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Carbamate kinasePRO_0000185149Add
BLAST

Proteomic databases

PRIDEiP95474.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi186497.PF0676.

Structurei

Secondary structure

1
314
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 85
Helixi11 – 133
Helixi23 – 4220
Beta strandi46 – 505
Helixi54 – 7118
Helixi78 – 10326
Beta strandi110 – 1134
Beta strandi116 – 1194
Helixi124 – 1263
Beta strandi131 – 1377
Helixi139 – 14911
Beta strandi152 – 1554
Beta strandi161 – 1655
Beta strandi170 – 1734
Helixi176 – 1849
Beta strandi188 – 1903
Helixi193 – 1953
Beta strandi197 – 2026
Beta strandi205 – 2084
Helixi215 – 22511
Beta strandi229 – 23911
Turni243 – 2453
Beta strandi254 – 2563
Helixi257 – 2659
Turni271 – 2733
Helixi274 – 28714
Beta strandi290 – 2967
Helixi297 – 2993
Helixi300 – 3045
Beta strandi307 – 3137

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E19X-ray1.50A/B1-314[»]
ProteinModelPortaliP95474.
SMRiP95474. Positions 2-314.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP95474.

Family & Domainsi

Sequence similaritiesi

Belongs to the carbamate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0549.
HOGENOMiHOG000277403.
KOiK00926.
OMAiKGTYEEQ.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR003964. Carb_kinase.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000723. Carbamate_kin. 1 hit.
PRINTSiPR01469. CARBMTKINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00746. arcC. 1 hit.

Sequencei

Sequence statusi: Complete.

P95474-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKRVVIALG GNALQQRGQK GSYEEMMDNV RKTARQIAEI IARGYEVVIT
60 70 80 90 100
HGNGPQVGSL LLHMDAGQAT YGIPAQPMDV AGAMSQGWIG YMIQQALKNE
110 120 130 140 150
LRKRGMEKKV VTIITQTIVD KNDPAFQNPT KPVGPFYDEE TAKRLAREKG
160 170 180 190 200
WIVKEDSGRG WRRVVPSPDP KGHVEAETIK KLVERGVIVI ASGGGGVPVI
210 220 230 240 250
LEDGEIKGVE AVIDKDLAGE KLAEEVNADI FMILTDVNGA ALYYGTEKEQ
260 270 280 290 300
WLREVKVEEL RKYYEEGHFK AGSMGPKVLA AIRFIEWGGE RAIIAHLEKA
310
VEALEGKTGT QVLP
Length:314
Mass (Da):34,429
Last modified:May 1, 1997 - v1
Checksum:iB4496B827581EF42
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09829 Genomic DNA. Translation: CAA70972.1.
AB016521 Genomic DNA. Translation: BAA32017.1.
AE009950 Genomic DNA. Translation: AAL80800.1.
PIRiT43855.
RefSeqiNP_578405.1. NC_003413.1.
WP_011011798.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80800; AAL80800; PF0676.
GeneIDi1468522.
KEGGipfu:PF0676.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09829 Genomic DNA. Translation: CAA70972.1 .
AB016521 Genomic DNA. Translation: BAA32017.1 .
AE009950 Genomic DNA. Translation: AAL80800.1 .
PIRi T43855.
RefSeqi NP_578405.1. NC_003413.1.
WP_011011798.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1E19 X-ray 1.50 A/B 1-314 [» ]
ProteinModelPortali P95474.
SMRi P95474. Positions 2-314.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 186497.PF0676.

Proteomic databases

PRIDEi P95474.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL80800 ; AAL80800 ; PF0676 .
GeneIDi 1468522.
KEGGi pfu:PF0676.

Phylogenomic databases

eggNOGi COG0549.
HOGENOMi HOG000277403.
KOi K00926.
OMAi KGTYEEQ.

Miscellaneous databases

EvolutionaryTracei P95474.

Family and domain databases

Gene3Di 3.40.1160.10. 1 hit.
InterProi IPR001048. Asp/Glu/Uridylate_kinase.
IPR003964. Carb_kinase.
[Graphical view ]
Pfami PF00696. AA_kinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000723. Carbamate_kin. 1 hit.
PRINTSi PR01469. CARBMTKINASE.
SUPFAMi SSF53633. SSF53633. 1 hit.
TIGRFAMsi TIGR00746. arcC. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The carbamate kinase-like carbamoyl phosphate synthetase of the hyperthermophilic archaeon Pyrococcus furiosus, a missing link in the evolution of carbamoyl phosphate biosynthesis."
    Durbecq V., Legrain C., Roovers M., Pierard A., Glansdorff N.
    Proc. Natl. Acad. Sci. U.S.A. 94:12803-12808(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  2. "Pfu helicase locus."
    Kanai A., Oida H., Yabe T., Hihara S., Doi H.
    Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  3. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  4. "The 1.5-A resolution crystal structure of the carbamate kinase-like carbamoyl phosphate synthetase from the hyperthermophilic Archaeon Pyrococcus furiosus, bound to ADP, confirms that this thermostable enzyme is a carbamate kinase, and provides insight into substrate binding and stability in carbamate kinases."
    Ramon-Maiques S., Marina A., Uriarte M., Fita I., Rubio V.
    J. Mol. Biol. 299:463-476(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS), FUNCTION.
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Entry informationi

Entry nameiCPKA_PYRFU
AccessioniPrimary (citable) accession number: P95474
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: May 1, 1997
Last modified: October 29, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3