P95474 (CPKA_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbamate kinase
EC=2.7.2.2

Alternative name(s):
Carbamate kinase-like carbamoylphosphate synthase

Gene names

Name:	cpkA
Synonyms:	cpa
Ordered Locus Names:	PF0676

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus furiosus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Carbamate kinase that plays a biosynthetic role in that it produces carbamoyl-phosphate. Ref.4
Catalytic activity	ATP + NH3 + CO2 = ADP + carbamoyl phosphate.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the carbamate kinase family.
Biophysicochemical properties	Temperature dependence: 50% activity is retained after 1 hour at 100 degrees Celsius or 3 hours at 95 degrees Celsius.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arginine metabolic process Inferred from electronic annotation. Source: InterPro cellular amino acid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 314

314

Carbamate kinase

PRO_0000185149

Secondary structure

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314

Helix Strand Turn

Details...

Beta strand

4 – 8

5

Helix

11 – 13

3

Helix

23 – 42

20

Beta strand

46 – 50

5

Helix

54 – 71

18

Helix

78 – 103

26

Beta strand

110 – 113

4

Beta strand

116 – 119

4

Helix

124 – 126

3

Beta strand

131 – 137

7

Helix

139 – 149

11

Beta strand

152 – 155

4

Beta strand

161 – 165

5

Beta strand

170 – 173

4

Helix

176 – 184

9

Beta strand

188 – 190

3

Helix

193 – 195

3

Beta strand

197 – 202

6

Beta strand

205 – 208

4

Helix

215 – 225

11

Beta strand

229 – 239

11

Turn

243 – 245

3

Beta strand

254 – 256

3

Helix

257 – 265

9

Turn

271 – 273

3

Helix

274 – 287

14

Beta strand

290 – 296

7

Helix

297 – 299

3

Helix

300 – 304

5

Beta strand

307 – 313

7

Sequences

	Sequence		Length	Mass (Da)
	P95474 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: B4496B827581EF42	FASTA	314	34,429
	10 20 30 40 50 60 MGKRVVIALG GNALQQRGQK GSYEEMMDNV RKTARQIAEI IARGYEVVIT HGNGPQVGSL 70 80 90 100 110 120 LLHMDAGQAT YGIPAQPMDV AGAMSQGWIG YMIQQALKNE LRKRGMEKKV VTIITQTIVD 130 140 150 160 170 180 KNDPAFQNPT KPVGPFYDEE TAKRLAREKG WIVKEDSGRG WRRVVPSPDP KGHVEAETIK 190 200 210 220 230 240 KLVERGVIVI ASGGGGVPVI LEDGEIKGVE AVIDKDLAGE KLAEEVNADI FMILTDVNGA 250 260 270 280 290 300 ALYYGTEKEQ WLREVKVEEL RKYYEEGHFK AGSMGPKVLA AIRFIEWGGE RAIIAHLEKA 310 VEALEGKTGT QVLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The carbamate kinase-like carbamoyl phosphate synthetase of the hyperthermophilic archaeon Pyrococcus furiosus, a missing link in the evolution of carbamoyl phosphate biosynthesis." Durbecq V., Legrain C., Roovers M., Pierard A., Glansdorff N. Proc. Natl. Acad. Sci. U.S.A. 94:12803-12808(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]	"Pfu helicase locus." Kanai A., Oida H., Yabe T., Hihara S., Doi H. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[3]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[4]	"The 1.5-A resolution crystal structure of the carbamate kinase-like carbamoyl phosphate synthetase from the hyperthermophilic Archaeon Pyrococcus furiosus, bound to ADP, confirms that this thermostable enzyme is a carbamate kinase, and provides insight into substrate binding and stability in carbamate kinases." Ramon-Maiques S., Marina A., Uriarte M., Fita I., Rubio V. J. Mol. Biol. 299:463-476(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS), FUNCTION. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y09829 Genomic DNA. Translation: CAA70972.1.
AB016521 Genomic DNA. Translation: BAA32017.1.
AE009950 Genomic DNA. Translation: AAL80800.1.

PIR

T43855.

RefSeq

NP_578405.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E19	X-ray	1.50	A/B	1-314	[»]

ProteinModelPortal

P95474.

SMR

P95474. Positions 2-314.

ModBase

Search...

Protein-protein interaction databases

STRING

186497.PF0676.

Proteomic databases

PRIDE

P95474.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAL80800; AAL80800; PF0676.

GeneID

1468522.

KEGG

pfu:PF0676.

Phylogenomic databases

eggNOG

COG0549.

HOGENOM

HOG000277403.

KO

K00926.

OMA

ADIFMIL.

ProtClustDB

PRK12454.

Family and domain databases

Gene3D

3.40.1160.10. 1 hit.

InterPro

IPR001048. Asp/Glu/Uridylate_kinase.
IPR003964. Bac_carb_kinase.
[Graphical view]

Pfam

PF00696. AA_kinase. 1 hit.
[Graphical view]

PIRSF

PIRSF000723. Carbamate_kin. 1 hit.

PRINTS

PR01469. CARBMTKINASE.

SUPFAM

SSF53633. Aa_kinase. 1 hit.

TIGRFAMs

TIGR00746. arcC. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P95474.

Entry information

Entry name

CPKA_PYRFU

Accession

Primary (citable) accession number: P95474

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program