P95377 (LPXD_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	NMB0180

Organism

Neisseria meningitidis serogroup B

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000059684
Sites
	Active site	241	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	1 – 3	3	MIP → VAV in AAC45422. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P95377 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: F555038EEAE229A8	FASTA	348	36,449
	10 20 30 40 50 60 MIPATYTLSQ ITARLGGEWR GEDISVTAVR PLADAQAEHI SFLANPKYKA EVHDSSAGAV 70 80 90 100 110 120 IVSAKAADGF EGRNLIVADD PYLYFAKVAR LFSPVVKARG GIHPTAVVEP GATVPTSCEI 130 140 150 160 170 180 GANVYIGANT VLGEGCRILA NAVVQHDCKL GDEVVLHPNA VVYYGCTLGR RVEIHSGAVI 190 200 210 220 230 240 GADGFGLAFA DDSWFKIPQT GAVTLGDDVE IGSNTNIDRG AMSDTTVGNG TKIDNQVQIG 250 260 270 280 290 300 HNCKIGSHTV IAAKTGISGS VTIGSYCIIG GGVGTVGHIE IADKTTIGGG TSVTHSITES 310 320 330 340 GKHLAGIFPM STHKEWARNA VYIHRLSEMN KRLKTLEQQL SDAGQDSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the Neisseria meningitidis lpxD-fabZ-lpxA gene cluster involved in lipid A biosynthesis." Steeghs L., Jennings M.P., Poolman J.T., Der Ley P. Gene 190:263-270(1997) [PubMed: 9197543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MC58 / Serogroup B.
[2]	"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U79481 Genomic DNA. Translation: AAC45422.1. AE002098 Genomic DNA. Translation: AAF40637.1.
PIR	E81228.
RefSeq	NP_273238.1. NC_003112.2.
3D structure databases
ProteinModelPortal	P95377.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000009922; EBNEIP00000009542; EBNEIG00000009922.
GeneID	902287.
GenomeReviews	Gene locus NMB0180 in contig AE002098_GR.
KEGG	nme:NMB0180.
NMPDR	fig|122586.1.peg.172.
PATRIC	20355385. VBINeiMen85645_0222.
TIGR	NMB0180.
Phylogenomic databases
GeneTree	EBGT00050000020532.
HOGENOM	HBG469615.
OMA	CFVGKNT.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0180-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_NEIMB

Accession

Primary (citable) accession number: P95377
Secondary accession number(s): Q9K1H2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 23, 2002
Last modified:	January 25, 2012
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program