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P95370

- CYSG_NEIMB

UniProt

P95370 - CYSG_NEIMB

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Protein

Siroheme synthase

Gene
cysG, cysG1, NMB1156
cysG2, NMB1194
Organism
Neisseria meningitidis serogroup B (strain MC58)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei226 – 2261S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei249 – 2491Proton acceptor By similarity
Active sitei271 – 2711Proton donor By similarity
Binding sitei307 – 3071S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei384 – 3841S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei413 – 4131S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NAD By similarity
Nucleotide bindingi43 – 442NAD By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciNMEN122586:GHGG-1192-MONOMER.
NMEN122586:GHGG-1229-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthase
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen III methylase
Alternative name(s):
SUMT
Uroporphyrinogen III methylase
Short name:
UROM
Precorrin-2 dehydrogenase (EC:1.3.1.76)
Sirohydrochlorin ferrochelatase (EC:4.99.1.4)
Gene namesi
Name:cysG
Synonyms:cysG1
Ordered Locus Names:NMB1156
AND
Name:cysG2
Ordered Locus Names:NMB1194
OrganismiNeisseria meningitidis serogroup B (strain MC58)
Taxonomic identifieri122586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
ProteomesiUP000000425: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483Siroheme synthaseUniRule annotationPRO_0000150382Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi122586.NMB1194.

Structurei

3D structure databases

ProteinModelPortaliP95370.
SMRiP95370. Positions 1-458.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 203203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase By similarityAdd
BLAST
Regioni217 – 483267Uroporphyrinogen-III C-methyltransferase By similarityAdd
BLAST
Regioni302 – 3043S-adenosyl-L-methionine binding By similarity
Regioni332 – 3332S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

In the C-terminal section; belongs to the precorrin methyltransferase family.

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiNRVGQAY.
OrthoDBiEOG6DRPFR.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P95370-1 [UniParc]FASTAAdd to Basket

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MNYFPIFANL AGRPVLVVGG GAVAARKISL LLKAGAEVRV AAKHLNAELS    50
ALAAENKILW LAEEFRAEHI RTVFLIIAAS SDQALNRRVF HLAESCQKPV 100
NVVDDRDHCS FIFPSVIDRN PVQIAVSSSG SAPVLARLLR ERLEALLPPS 150
LGDMAEISGR WRDAVKGKLK SVTERRRFWE KQFNGRFAAL VKNRQNTLAE 200
RELAGQLEQS RQNDQGGSVS LVGAGPGDAG LLTLKGLQEI QQADVVLYDA 250
LVSDGILSLV RRDAERIFVG KRARGERTPQ EDTNALMVRL AREGRRVVRL 300
KGGDPFVFGR GGEELETLAR HQIPFSVVPG ITAAVGATAY AGIPLTHRDY 350
AQSAVFVTGH RKADAPDIEW QTLARSRQTL VIYMGALKAA LIAERLQQHG 400
RSPDTPAAVI SQGTLPAQKT ATGTLANLAE LAETAPNPAL IVIGEVVGLH 450
EKLAWFGENG EGENRVGQTY PALGGLNAGQ RAA 483
Length:483
Mass (Da):52,111
Last modified:December 1, 2000 - v3
Checksum:i279C4F458B19582F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti475 – 4839GLNAGQRAA → D in CAA71251. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y10177 Genomic DNA. Translation: CAA71251.1.
AE002098 Genomic DNA. Translation: AAF41542.1.
AE002098 Genomic DNA. Translation: AAF41577.1.
PIRiD81111.
T46867.
RefSeqiNP_274184.1. NC_003112.2.
NP_274220.1. NC_003112.2.

Genome annotation databases

EnsemblBacteriaiAAF41542; AAF41542; NMB1156.
AAF41577; AAF41577; NMB1194.
GeneIDi903577.
903614.
KEGGinme:NMB1156.
nme:NMB1194.
PATRICi20357891. VBINeiMen85645_1461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y10177 Genomic DNA. Translation: CAA71251.1 .
AE002098 Genomic DNA. Translation: AAF41542.1 .
AE002098 Genomic DNA. Translation: AAF41577.1 .
PIRi D81111.
T46867.
RefSeqi NP_274184.1. NC_003112.2.
NP_274220.1. NC_003112.2.

3D structure databases

ProteinModelPortali P95370.
SMRi P95370. Positions 1-458.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 122586.NMB1194.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAF41542 ; AAF41542 ; NMB1156 .
AAF41577 ; AAF41577 ; NMB1194 .
GeneIDi 903577.
903614.
KEGGi nme:NMB1156.
nme:NMB1194.
PATRICi 20357891. VBINeiMen85645_1461.

Phylogenomic databases

eggNOGi COG0007.
HOGENOMi HOG000290518.
KOi K02302.
OMAi NRVGQAY.
OrthoDBi EOG6DRPFR.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00148 ; UER00222 .
UPA00262 ; UER00211 .
UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci NMEN122586:GHGG-1192-MONOMER.
NMEN122586:GHGG-1229-MONOMER.

Family and domain databases

Gene3Di 1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01646. Siroheme_synth.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sirohaem synthase from Neisseria meningitidis."
    McVeigh T., Wilkie S.E., Woodcock S.C., Mortuza G., Peters S.E., Warren M.J.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MC58.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MC58.

Entry informationi

Entry nameiCYSG_NEIMB
AccessioniPrimary (citable) accession number: P95370
Secondary accession number(s): Q9JQK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: December 1, 2000
Last modified: June 11, 2014
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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