Reviewed, UniProtKB/Swiss-Prot P95331 (FUMC_MYXXD)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Synonyms:	fhy
Ordered Locus Names:	MXAN_6439

Organism

Myxococcus xanthus (strain DK 1622) [Complete proteome] [HAMAP]

Taxonomic identifier

246197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Myxococcus

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161290
Regions
	Region	131 – 134	4	B site By similarity
	Region	141 – 143	3	Substrate binding By similarity
Sites
	Binding site	102	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P95331-1 [UniParc]. Last modified June 1, 1998. Version 2. Checksum: 9496DDD6C1690C55	FASTA	466	49,913
	10 20 30 40 50 60 MSTKNVRTEK DTFGPIDVPA DRLWGAQTQR SLQNFAISTE RMPLALIRAL VLVKKAAARV 70 80 90 100 110 120 NVENGSLAKE KGEAIIRAAD EVLAGQHDAE FPLSVWQTGS GTQTNMNTNE VLANRASELL 130 140 150 160 170 180 GGERGERRKV HPNDDVNKGQ SSNDVFPTAM SVAAVAAITE HVLPELKALR DVLAQKARAF 190 200 210 220 230 240 HDVVKVGRTH LQDATPLTLG QEVGGFVAQL DHAKGHLERT LPHLLELALG GTAVGTGLNA 250 260 270 280 290 300 PKGYAERVAQ ELAQLTGHPF VTAPNKFEAL AANDALVQAH GALKGLAAVL FKVANDVRWL 310 320 330 340 350 360 SSGPRSGLAE ITIPENEPGS SIMPGKVNPT QSEALTMLCA QVMGNDVAVT VGGASGNFQL 370 380 390 400 410 420 NVFKPLIAHN LLQSCRLLAD GMRSFRLHCA VGIEPNRPRI QENLERSLML VTALNPHIGY 430 440 450 460 DNAAKIAKTA HRDGTTLKET AVALGLVTPE QFDQWVRPED MTGHKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Myxococcus xanthus fumarate hydratase, major proteosome subunit, and acyl-CoA oxidase genes." Salmi D., Creighton C., Youderian P. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Evolution of sensory complexity recorded in a myxobacterial genome." Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J., Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G., Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A. , Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B. Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006) [PubMed: 17015832] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF013216 Genomic DNA. Translation: AAB97818.1. CP000113 Genomic DNA. Translation: ABF86177.1.
RefSeq	YP_634563.1.
3D structure databases
HSSP	HSSP built from PDB template 1FUR based on UniProtKB P05042.
SMR	P95331. Positions 6-461.
ModBase	Search...
Protein-protein interaction databases
STRING	P95331.
Genome annotation databases
GeneID	4105200.
GenomeReviews	Gene locus MXAN_6439 in contig CP000113_GR.
KEGG	mxa:MXAN_6439.
TIGR	MXAN_6439.
Phylogenomic databases
HOGENOM	P95331.
OMA	GSQGHFE.
Enzyme and pathway databases
BioCyc	MXAN246197:MXAN_6439-MON.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_MYXXD

Accession

Primary (citable) accession number: P95331
Secondary accession number(s): Q1CYG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 1998
Last modified:	November 3, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)