P95329 (MASY_MYXXD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase
EC=2.3.3.9

Gene names

Name:	mls
Ordered Locus Names:	MXAN_6441

Organism

Myxococcus xanthus (strain DK 1622) [Complete proteome] [HAMAP]

Taxonomic identifier

246197 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Myxococcus › Myxococcus xanthus

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 541	541	Malate synthase		PRO_0000166876
Sites
	Active site	172	1	Proton acceptor By similarity
	Active site	452	1	Proton donor By similarity
Experimental info
	Sequence conflict	95	1	D → E in AAB97829. Ref.1
	Sequence conflict	385	1	I → L in AAB97829. Ref.1
	Sequence conflict	398	1	N → D in AAB97829. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P95329 [UniParc]. Last modified September 19, 2006. Version 2. Checksum: E25A8236686AA626	FASTA	541	60,509
	10 20 30 40 50 60 MSDQAPSVKP PAFGPGVVVK GTWHPDYAEV LTPEALEFVA KLARNFGERR LALLERRKTV 70 80 90 100 110 120 QAAWSKGARP HFLPETKAVR EGDWTVAPLP ADLQDRRVEI TGPVDRKMVI NALNSGANVF 130 140 150 160 170 180 MADFEDANSP TWDNVVRGQI NLRDAVRGTI SFTAENGKHY ALNPKRAVLF VRPRGWHLPE 190 200 210 220 230 240 RHIEIDGKPI SGSLLDFGLF FFHNAREQLA RGTGPYFYLP KMQSHLEARL WNDVFHLAQA 250 260 270 280 290 300 ELGIPRGTIK ATVLIETLPA AFEMDEILYE LREHSAGLNC GRWDYIFSFI KTLQSDTRVV 310 320 330 340 350 360 LPDRGQVTMD KAFLNAYSQL LIQTCHRRNV HAMGGMAAFI PIKGDAAAND AVMDKVRADK 370 380 390 400 410 420 LREVKNGHDG TWVAHPGLVE IARDIFDSHM KGPNQLSNKR EDVKIGEAEL LKVPSGTRTE 430 440 450 460 470 480 EGLRHNIRVG IQYTAAWLGG LGCVPLYNLM EDAATAEISR AQVWQWIHHG ASLEDGRKVT 490 500 510 520 530 540 LTLFRDALGE EMGRLEKEGA KERYGAAHLE RARVLFEQLS TAGTFEDFLT LPAYDALEAQ R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Myxococcus xanthus fumarate hydratase, major proteosome subunit, and acyl-CoA oxidase genes." Salmi D., Creighton C., Youderian P. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Evolution of sensory complexity recorded in a myxobacterial genome." Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J., Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G., Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A. , Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B. Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DK 1622.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF013216 Genomic DNA. Translation: AAB97829.1. CP000113 Genomic DNA. Translation: ABF86861.1.
RefSeq	YP_634565.1. NC_008095.1.
3D structure databases
ProteinModelPortal	P95329.
ModBase	Search...
Protein-protein interaction databases
STRING	246197.MXAN_6441.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABF86861; ABF86861; MXAN_6441.
GeneID	4105164.
KEGG	mxa:MXAN_6441.
PATRIC	22655385. VBIMyxXan43560_6336.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HOG000238464.
KO	K01638.
OMA	GFDGTWV.
ProtClustDB	PRK09255.
Enzyme and pathway databases
BioCyc	MXAN246197:GIWU-6390-MONOMER.
UniPathway	UPA00703; UER00720.
Family and domain databases
InterPro	IPR011076. Malate_synth-like. IPR006252. Malate_synthA. IPR001465. Malate_synthase. IPR019830. Malate_synthase_CS. [Graphical view]
PANTHER	PTHR21631:SF1. PTHR21631:SF1. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001363. Malate_synth. 1 hit.
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MASY_MYXXD

Accession

Primary (citable) accession number: P95329
Secondary accession number(s): Q1CYF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	September 19, 2006
Last modified:	May 1, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program