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P95329 (MASY_MYXXD) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase

EC=2.3.3.9
Gene names
Name:mls
Ordered Locus Names:MXAN_6441
OrganismMyxococcus xanthus (strain DK 1622) [Complete proteome] [HAMAP]
Taxonomic identifier246197 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeMyxococcus

Protein attributes

Sequence length541 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the malate synthase family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 541541Malate synthase
PRO_0000166876

Sites

Active site1721Proton acceptor By similarity
Active site4521Proton donor By similarity

Experimental info

Sequence conflict951D → E in AAB97829. Ref.1
Sequence conflict3851I → L in AAB97829. Ref.1
Sequence conflict3981N → D in AAB97829. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P95329 [UniParc].

Last modified September 19, 2006. Version 2.
Checksum: E25A8236686AA626

FASTA54160,509
        10         20         30         40         50         60 
MSDQAPSVKP PAFGPGVVVK GTWHPDYAEV LTPEALEFVA KLARNFGERR LALLERRKTV 

        70         80         90        100        110        120 
QAAWSKGARP HFLPETKAVR EGDWTVAPLP ADLQDRRVEI TGPVDRKMVI NALNSGANVF 

       130        140        150        160        170        180 
MADFEDANSP TWDNVVRGQI NLRDAVRGTI SFTAENGKHY ALNPKRAVLF VRPRGWHLPE 

       190        200        210        220        230        240 
RHIEIDGKPI SGSLLDFGLF FFHNAREQLA RGTGPYFYLP KMQSHLEARL WNDVFHLAQA 

       250        260        270        280        290        300 
ELGIPRGTIK ATVLIETLPA AFEMDEILYE LREHSAGLNC GRWDYIFSFI KTLQSDTRVV 

       310        320        330        340        350        360 
LPDRGQVTMD KAFLNAYSQL LIQTCHRRNV HAMGGMAAFI PIKGDAAAND AVMDKVRADK 

       370        380        390        400        410        420 
LREVKNGHDG TWVAHPGLVE IARDIFDSHM KGPNQLSNKR EDVKIGEAEL LKVPSGTRTE 

       430        440        450        460        470        480 
EGLRHNIRVG IQYTAAWLGG LGCVPLYNLM EDAATAEISR AQVWQWIHHG ASLEDGRKVT 

       490        500        510        520        530        540 
LTLFRDALGE EMGRLEKEGA KERYGAAHLE RARVLFEQLS TAGTFEDFLT LPAYDALEAQ 


R 

« Hide

References

« Hide 'large scale' references
[1]"Myxococcus xanthus fumarate hydratase, major proteosome subunit, and acyl-CoA oxidase genes."
Salmi D., Creighton C., Youderian P.
Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Evolution of sensory complexity recorded in a myxobacterial genome."
Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J., Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G., Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A. expand/collapse author list , Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B.
Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DK 1622.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF013216 Genomic DNA. Translation: AAB97829.1.
CP000113 Genomic DNA. Translation: ABF86861.1.
RefSeqYP_634565.1. NC_008095.1.

3D structure databases

ProteinModelPortalP95329.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246197.MXAN_6441.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABF86861; ABF86861; MXAN_6441.
GeneID4105164.
KEGGmxa:MXAN_6441.
PATRIC22655385. VBIMyxXan43560_6336.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2225.
HOGENOMHOG000238464.
KOK01638.
OMAPGTIKCT.
OrthoDBEOG69WFFX.
ProtClustDBPRK09255.

Enzyme and pathway databases

BioCycMXAN246197:GIWU-6390-MONOMER.
UniPathwayUPA00703; UER00720.

Family and domain databases

InterProIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PANTHERPTHR21631:SF1. PTHR21631:SF1. 1 hit.
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001363. Malate_synth. 1 hit.
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01344. malate_syn_A. 1 hit.
PROSITEPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMASY_MYXXD
AccessionPrimary (citable) accession number: P95329
Secondary accession number(s): Q1CYF8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: September 19, 2006
Last modified: November 13, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways