Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Malate synthase

Gene

mls

Organism
Myxococcus xanthus (strain DK 1622)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase (mls)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei172 – 1721Proton acceptorBy similarity
Active sitei452 – 4521Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciMXAN246197:GIWU-6390-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase (EC:2.3.3.9)
Gene namesi
Name:mls
Ordered Locus Names:MXAN_6441
OrganismiMyxococcus xanthus (strain DK 1622)
Taxonomic identifieri246197 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeMyxococcus
ProteomesiUP000002402 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 541541Malate synthasePRO_0000166876Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246197.MXAN_6441.

Structurei

3D structure databases

ProteinModelPortaliP95329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000238464.
KOiK01638.
OMAiMMINALN.
OrthoDBiEOG69WFFX.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P95329-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQAPSVKP PAFGPGVVVK GTWHPDYAEV LTPEALEFVA KLARNFGERR
60 70 80 90 100
LALLERRKTV QAAWSKGARP HFLPETKAVR EGDWTVAPLP ADLQDRRVEI
110 120 130 140 150
TGPVDRKMVI NALNSGANVF MADFEDANSP TWDNVVRGQI NLRDAVRGTI
160 170 180 190 200
SFTAENGKHY ALNPKRAVLF VRPRGWHLPE RHIEIDGKPI SGSLLDFGLF
210 220 230 240 250
FFHNAREQLA RGTGPYFYLP KMQSHLEARL WNDVFHLAQA ELGIPRGTIK
260 270 280 290 300
ATVLIETLPA AFEMDEILYE LREHSAGLNC GRWDYIFSFI KTLQSDTRVV
310 320 330 340 350
LPDRGQVTMD KAFLNAYSQL LIQTCHRRNV HAMGGMAAFI PIKGDAAAND
360 370 380 390 400
AVMDKVRADK LREVKNGHDG TWVAHPGLVE IARDIFDSHM KGPNQLSNKR
410 420 430 440 450
EDVKIGEAEL LKVPSGTRTE EGLRHNIRVG IQYTAAWLGG LGCVPLYNLM
460 470 480 490 500
EDAATAEISR AQVWQWIHHG ASLEDGRKVT LTLFRDALGE EMGRLEKEGA
510 520 530 540
KERYGAAHLE RARVLFEQLS TAGTFEDFLT LPAYDALEAQ R
Length:541
Mass (Da):60,509
Last modified:September 19, 2006 - v2
Checksum:iE25A8236686AA626
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 951D → E in AAB97829 (Ref. 1) Curated
Sequence conflicti385 – 3851I → L in AAB97829 (Ref. 1) Curated
Sequence conflicti398 – 3981N → D in AAB97829 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013216 Genomic DNA. Translation: AAB97829.1.
CP000113 Genomic DNA. Translation: ABF86861.1.
RefSeqiWP_011556373.1. NC_008095.1.
YP_634565.1. NC_008095.1.

Genome annotation databases

EnsemblBacteriaiABF86861; ABF86861; MXAN_6441.
KEGGimxa:MXAN_6441.
PATRICi22655385. VBIMyxXan43560_6336.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013216 Genomic DNA. Translation: AAB97829.1.
CP000113 Genomic DNA. Translation: ABF86861.1.
RefSeqiWP_011556373.1. NC_008095.1.
YP_634565.1. NC_008095.1.

3D structure databases

ProteinModelPortaliP95329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246197.MXAN_6441.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF86861; ABF86861; MXAN_6441.
KEGGimxa:MXAN_6441.
PATRICi22655385. VBIMyxXan43560_6336.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000238464.
KOiK01638.
OMAiMMINALN.
OrthoDBiEOG69WFFX.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciMXAN246197:GIWU-6390-MONOMER.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Myxococcus xanthus fumarate hydratase, major proteosome subunit, and acyl-CoA oxidase genes."
    Salmi D., Creighton C., Youderian P.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DK 1622.

Entry informationi

Entry nameiMASY_MYXXD
AccessioniPrimary (citable) accession number: P95329
Secondary accession number(s): Q1CYF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: September 19, 2006
Last modified: May 27, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.