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Protein

Epoxide hydrolase B

Gene

MT1988

Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Could be involved in detoxification of extraneous host-cell epoxides. Catalyzes the hydrolysis of small aromatic epoxide-containing substrates such as trans-1,3-diphenylpropene oxide, trans and cis-stilbene oxide, and terpenoid epoxide.1 Publication

Catalytic activityi

An epoxide + H2O = a glycol.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104Nucleophile1 Publication1
Sitei164Contributes to the formation of an oxyanion binding site for the epoxide oxygen of substrate1 Publication1
Sitei272Contributes to the formation of an oxyanion binding site for the epoxide oxygen of substrate1 Publication1
Sitei302Plays an orienting role for the imidazole group of His-3331 Publication1
Active sitei333Proton acceptor1 Publication1

GO - Molecular functioni

  • epoxide hydrolase B activity Source: MTBBASE
  • protein homodimerization activity Source: MTBBASE

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processDetoxification

Protein family/group databases

ESTHERimyctu-ephB. Epoxide_hydrolase.
MEROPSiS33.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Epoxide hydrolase B1 Publication (EC:3.3.2.101 Publication)
Short name:
EHB1 Publication
Gene namesi
Ordered Locus Names:MT1988
OrganismiMycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Taxonomic identifieri83331 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001020 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1795155.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004385871 – 356Epoxide hydrolase BAdd BLAST356

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: MTBBASE

Chemistry databases

BindingDBiP95276.

Structurei

Secondary structure

1356
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 8Combined sources4
Beta strandi10 – 12Combined sources3
Beta strandi15 – 20Combined sources6
Beta strandi29 – 33Combined sources5
Helixi40 – 43Combined sources4
Turni44 – 46Combined sources3
Helixi47 – 52Combined sources6
Beta strandi56 – 60Combined sources5
Helixi74 – 77Combined sources4
Helixi79 – 92Combined sources14
Beta strandi98 – 103Combined sources6
Helixi106 – 116Combined sources11
Helixi118 – 120Combined sources3
Beta strandi121 – 129Combined sources9
Helixi133 – 135Combined sources3
Helixi149 – 154Combined sources6
Beta strandi155 – 157Combined sources3
Beta strandi159 – 163Combined sources5
Helixi164 – 170Combined sources7
Helixi173 – 179Combined sources7
Helixi182 – 192Combined sources11
Helixi195 – 203Combined sources9
Helixi217 – 219Combined sources3
Beta strandi227 – 229Combined sources3
Helixi234 – 237Combined sources4
Beta strandi246 – 248Combined sources3
Helixi250 – 263Combined sources14
Helixi266 – 273Combined sources8
Helixi275 – 281Combined sources7
Helixi283 – 285Combined sources3
Beta strandi294 – 299Combined sources6
Helixi303 – 307Combined sources5
Helixi309 – 313Combined sources5
Helixi315 – 318Combined sources4
Beta strandi322 – 328Combined sources7
Helixi335 – 338Combined sources4
Helixi340 – 352Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E3JX-ray2.10A2-356[»]
2ZJFX-ray2.40A2-356[»]
ProteinModelPortaliP95276.
SMRiP95276.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP95276.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 129AB hydrolase-1Sequence analysisAdd BLAST102

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000028073.
OrthoDBiPOG091H0A6K.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiView protein in InterPro
IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
PfamiView protein in Pfam
PF00561. Abhydrolase_1. 1 hit.
PRINTSiPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 2 hits.

Sequencei

Sequence statusi: Complete.

P95276-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQVHRILNC RGTRIHAVAD SPPDQQGPLV VLLHGFPESW YSWRHQIPAL
60 70 80 90 100
AGAGYRVVAI DQRGYGRSSK YRVQKAYRIK ELVGDVVGVL DSYGAEQAFV
110 120 130 140 150
VGHDWGAPVA WTFAWLHPDR CAGVVGISVP FAGRGVIGLP GSPFGERRPS
160 170 180 190 200
DYHLELAGPG RVWYQDYFAV QDGIITEIEE DLRGWLLGLT YTVSGEGMMA
210 220 230 240 250
ATKAAVDAGV DLESMDPIDV IRAGPLCMAE GARLKDAFVY PETMPAWFTE
260 270 280 290 300
ADLDFYTGEF ERSGFGGPLS FYHNIDNDWH DLADQQGKPL TPPALFIGGQ
310 320 330 340 350
YDVGTIWGAQ AIERAHEVMP NYRGTHMIAD VGHWIQQEAP EETNRLLLDF

LGGLRP
Length:356
Mass (Da):39,297
Last modified:July 1, 1997 - v2
Checksum:i64D10EA23A0D57AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA. Translation: AAK46260.1.
PIRiF70636.
RefSeqiWP_003899091.1. NZ_KK341227.1.

Genome annotation databases

EnsemblBacteriaiAAK46260; AAK46260; MT1988.
KEGGimtc:MT1988.
PATRICifig|83331.31.peg.2142.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiEPHB_MYCTO
AccessioniPrimary (citable) accession number: P95276
Secondary accession number(s): F2GGS7, Q7D7R2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2016
Last sequence update: July 1, 1997
Last modified: June 7, 2017
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families