P95231 (CYSE_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine acetyltransferase
Short name=SAT
EC=2.3.1.30

Gene names

Name:	cysE
Ordered Locus Names:	Rv2335

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acetylation of serine by acetyl-CoA to produce O-acetylserine (OAS) By similarity.
Catalytic activity	Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.
Subcellular location	Cytoplasm By similarity.
Disruption phenotype	Strains lacking this gene are shown to be attenuated in a mouse tuberculosis model. Ref.2
Miscellaneous	Was identified as a high-confidence drug target.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cysteine biosynthetic process from serine Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	serine O-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Serine acetyltransferase		PRO_0000401135

Sequences

	Sequence		Length	Mass (Da)
	P95231 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 3C3E8F311DAB2D0F	FASTA	229	23,770
	10 20 30 40 50 60 MLTAMRGDIR AARERDPAAP TALEVIFCYP GVHAVWGHRL AHWLWQRGAR LLARAAAEFT 70 80 90 100 110 120 RILTGVDIHP GAVIGARVFI DHATGVVIGE TAEVGDDVTI YHGVTLGGSG MVGGKRHPTV 130 140 150 160 170 180 GDRVIIGAGA KVLGPIKIGE DSRIGANAVV VKPVPPSAVV VGVPGQVIGQ SQPSPGGPFD 190 200 210 220 WRLPDLVGAS LDSLLTRVAR LEALGGGPQA AGVIRPPEAG IWHGEDFSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Genetic requirements for mycobacterial survival during infection." Sassetti C.M., Rubin E.J. Proc. Natl. Acad. Sci. U.S.A. 100:12989-12994(2003) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE. Strain: ATCC 25618 / H37Rv.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842579 Genomic DNA. Translation: CAB06152.1. AL123456 Genomic DNA. Translation: CCP45123.1.
PIR	H70660.
RefSeq	NP_216851.1. NC_000962.3. YP_006515769.1. NC_018143.1.
3D structure databases
ProteinModelPortal	P95231.
SMR	P95231. Positions 5-160.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv2335.
Proteomic databases
PRIDE	P95231.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	13319040. 886012.
KEGG	mtu:Rv2335. mtv:RVBD_2335.
PATRIC	18153767. VBIMycTub87468_2608.
Organism-specific databases
TubercuList	Rv2335.
Phylogenomic databases
eggNOG	COG1045.
HOGENOM	HOG000049432.
KO	K00640.
OMA	TIKDNCL.
ProtClustDB	CLSK791792.
Enzyme and pathway databases
Reactome	REACT_27295. Mycobacterium tuberculosis biological processes.
UniPathway	UPA00136; UER00199.
Family and domain databases
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR005881. Ser_O-AcTrfase. IPR011004. Trimer_LpxA-like. [Graphical view]
Pfam	PF00132. Hexapep. 1 hit. [Graphical view]
PIRSF	PIRSF000441. CysE. 1 hit.
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01172. cysE. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSE_MYCTU

Accession

Primary (citable) accession number: P95231
Secondary accession number(s): L0TAX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 30, 2010
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program