Skip Header

Contribute Send feedback
Read comments (?) or add your own

P95114 (DDL_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase
EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Synonyms:ddlA
Ordered Locus Names:Rv2981c, MT3059
ORF Names:MTCY349.06
OrganismMycobacterium tuberculosis [Reference proteome] [HAMAP]
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell wall formation By similarity.

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177844

Regions

Domain156 – 363208ATP-grasp
Nucleotide binding184 – 23956ATP By similarity

Sites

Metal binding3181Magnesium or manganese 1 By similarity
Metal binding3301Magnesium or manganese 1 By similarity
Metal binding3301Magnesium or manganese 2 By similarity
Metal binding3321Magnesium or manganese 2 By similarity

Experimental info

Sequence conflict3651T → A in AAK47388. Ref.2

Secondary structure

1 ........................................................... 373
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P95114 [UniParc].

Last modified July 1, 1997. Version 2.
Checksum: FD7838E8B7526B53

FASTA37339,710
        10         20         30         40         50         60 
MSANDRRDRR VRVAVVFGGR SNEHAISCVS AGSILRNLDS RRFDVIAVGI TPAGSWVLTD 

        70         80         90        100        110        120 
ANPDALTITN RELPQVKSGS GTELALPADP RRGGQLVSLP PGAGEVLESV DVVFPVLHGP 

       130        140        150        160        170        180 
YGEDGTIQGL LELAGVPYVG AGVLASAVGM DKEFTKKLLA ADGLPVGAYA VLRPPRSTLH 

       190        200        210        220        230        240 
RQECERLGLP VFVKPARGGS SIGVSRVSSW DQLPAAVARA RRHDPKVIVE AAISGRELEC 

       250        260        270        280        290        300 
GVLEMPDGTL EASTLGEIRV AGVRGREDSF YDFATKYLDD AAELDVPAKV DDQVAEAIRQ 

       310        320        330        340        350        360 
LAIRAFAAID CRGLARVDFF LTDDGPVINE INTMPGFTTI SMYPRMWAAS GVDYPTLLAT 

       370 
MIETTLARGV GLH

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX842581 Genomic DNA. Translation: CAB05431.1.
AE000516 Genomic DNA. Translation: AAK47388.1.
AL123456 Genomic DNA. Translation: CCP45786.1.
PIRB70673.
RefSeqNP_217497.1. NC_000962.3.
NP_337574.1. NC_002755.2.
YP_006516437.1. NC_018143.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3LWBX-ray2.10A/B1-373[»]
ProteinModelPortalP95114.
SMRP95114. Positions 17-368.
ModBaseSearch...

Protein-protein interaction databases

STRING83332.Rv2981c.

Proteomic databases

PRIDEP95114.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK47388; AAK47388; MT3059.
GeneID13317778.
888415.
925214.
KEGGmtc:MT3059.
mtu:Rv2981c.
mtv:RVBD_2981c.
PATRIC18128492. VBIMycTub22151_3346.

Organism-specific databases

TubercuListRv2981c.

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMALLHGPFG.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-15228.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChEMBLCHEMBL2030.
EvolutionaryTraceP95114.

Entry information

Entry nameDDL_MYCTU
AccessionPrimary (citable) accession number: P95114
Secondary accession number(s): L0TBF6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents