DNA-binding protein HU homolog - Mycobacterium tuberculosis

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P95109 (DBH_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA-binding protein HU homolog

Alternative name(s):
21 kDa laminin-2-binding protein
28 kDa iron-regulated protein
Short name=Irep-28
Histone-like protein
Short name=Hlp

Gene names

Name:	hup
Synonyms:	hlp, hupB, lbp21
Ordered Locus Names:	Rv2986c, MT3064
ORF Names:	MTCY349.01

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions. Binds DNA non-specifically. Induces lymphoproliferation, particularly in health tuberculin reactors, and is immunogenic. Maybe involved in pathogenesis of inflammatory bowel disease (IBD) in patients with ulcerative colitis and Crohn disease (CD). Bound by anti-neutrophil cytoplasmic antibodies (pANCA), which are a hallmark of IBD. The binding is due to pANCA directed against HIST1H1D cross-reacting with DBH epitopes. In CD, target of a strong IgA response. Ref.1 Ref.6
Subcellular location	Secreted › cell wall Ref.5.
Induction	By iron limitation. Ref.5
Miscellaneous	Was identified as a high-confidence drug target.
Sequence similarities	Belongs to the bacterial histone-like protein family.

Ontologies

Keywords
Biological process	DNA condensation
Cellular component	Cell wall Secreted
Domain	Repeat
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA protection Inferred from direct assay PubMed 20824060. Source: MTBBASE cellular response to iron ion starvation Inferred from expression pattern Ref.5. Source: MTBBASE chromosome condensation Inferred from electronic annotation. Source: UniProtKB-KW growth Inferred from mutant phenotype PubMed 12657046. Source: MTBBASE nucleoid organization Inferred from direct assay PubMed 20824060. Source: MTBBASE
Cellular_component	cell wall Inferred from direct assay PubMed 15525680 PubMed 20825248. Source: MTBBASE extracellular region Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 14532352 PubMed 15525680. Source: MTBBASE
Molecular_function	damaged DNA binding Inferred from direct assay PubMed 20824060. Source: MTBBASE double-stranded DNA binding Inferred from direct assay PubMed 20824060. Source: MTBBASE supercoiled DNA binding Inferred from direct assay PubMed 20824060. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 214

214

DNA-binding protein HU homolog

PRO_0000104950

Regions

Region

1 – 90

Bacterial histone-like domain

Region

101 – 214

114

Degenerate repeats region

Experimental info

Sequence conflict

208

T → A no nucleotide entry Ref.1

Secondary structure

214

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P95109 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: CB09AF20FB353544
Show »

FASTA

214

22,187

        10         20         30         40         50         60 
MNKAELIDVL TQKLGSDRRQ ATAAVENVVD TIVRAVHKGD SVTITGFGVF EQRRRAARVA 

        70         80         90        100        110        120 
RNPRTGETVK VKPTSVPAFR PGAQFKAVVS GAQRLPAEGP AVKRGVGASA AKKVAKKAPA 

       130        140        150        160        170        180 
KKATKAAKKA ATKAPARKAA TKAPAKKAAT KAPAKKAVKA TKSPAKKVTK AVKKTAVKAS 

       190        200        210 
VRKAATKAPA KKAAAKRPAT KAPAKKATAR RGRK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel mycobacterial histone H1 homologue (HupB) as an antigenic target of pANCA monoclonal antibody and serum immunoglobulin A from patients with Crohn's disease." Cohavy O., Harth G., Horwitz M., Eggena M., Landers C., Sutton C., Targan S.R., Braun J. Infect. Immun. 67:6510-6517(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 35801 / TMC 107 / Erdman.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[4]	"Decreased infectivity despite unaltered C3 binding by a DeltahbhA mutant of Mycobacterium tuberculosis." Mueller-Ortiz S.L., Sepulveda E., Olsen M.R., Jagannath C., Wanger A.R., Norris S.J. Infect. Immun. 70:6751-6760(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 4-13 AND 73-85. Strain: ATCC 27294 / TMC 102 / H37Rv.
[5]	"Identification and characterization of a major cell wall-associated iron-regulated envelope protein (Irep-28) in Mycobacterium tuberculosis." Yeruva V.C., Duggirala S., Lakshmi V., Kolarich D., Altmann F., Sritharan M. Clin. Vaccine Immunol. 13:1137-1142(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-34; 87-94 AND 95-103, SUBCELLULAR LOCATION, INDUCTION. Strain: ATCC 27294 / TMC 102 / H37Rv.
[6]	"Identification of an immunogenic histone-like protein (HLPMt) of Mycobacterium tuberculosis." Prabhakar S., Annapurna P.S., Jain N.K., Dey A.B., Tyagi J.S., Prasad H.K. Tuber. Lung Dis. 79:43-53(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 71-86, FUNCTION. Strain: ATCC 25618 / H37Rv.
[7]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BX842581 Genomic DNA. Translation: CAB05427.1.
AE000516 Genomic DNA. Translation: AAK47393.1.
AL123456 Genomic DNA. Translation: CCP45791.1.

PIR

G70673.

RefSeq

NP_217502.1. NC_000962.3.
NP_337579.1. NC_002755.2.
YP_006516442.1. NC_018143.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3C4I	X-ray	2.04	A/B	1-99	[»]
4DKY	X-ray	2.48	A/B	1-100	[»]

ProteinModelPortal

P95109.

SMR

P95109. Positions 1-99.

ModBase

Search...

Protein-protein interaction databases

STRING

83332.Rv2986c.

Proteomic databases

PRIDE

P95109.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAK47393; AAK47393; MT3064.

GeneID

13317783.
888166.
925208.

KEGG

mtc:MT3064.
mtu:Rv2986c.
mtv:RVBD_2986c.

PATRIC

18128502. VBIMycTub22151_3351.

Organism-specific databases

TubercuList

Rv2986c.

Phylogenomic databases

eggNOG

COG0776.

HOGENOM

HOG000046977.

K03530.

OMA

VPTFRPG.

ProtClustDB

CLSK872125.

Family and domain databases

Gene3D

4.10.520.10. 1 hit.

InterPro

IPR023630. DNA-bd_HU/IHF.
IPR000119. Hist_DNA-bd_bac.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd_dom.
[Graphical view]

Pfam

PF00216. Bac_DNA_binding. 1 hit.
[Graphical view]

PRINTS

PR01727. DNABINDINGHU.

SMART

SM00411. BHL. 1 hit.
[Graphical view]

SUPFAM

SSF47729. IHF_like_DNA_bnd. 1 hit.

PROSITE

PS00045. HISTONE_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P95109.

Entry information

Entry name

DBH_MYCTU

Accession

Primary (citable) accession number: P95109
Secondary accession number(s): L0TBG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents