P95020 (NUSB_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N utilization substance protein B homolog
Short name=Protein NusB

Gene names

Name:	nusB
Ordered Locus Names:	Rv2533c, MT2608
ORF Names:	MTCY159.23

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the transcription termination process By similarity. HAMAP MF_00073
Subunit structure	Homodimer.
Miscellaneous	Was identified as a high-confidence drug target. HAMAP MF_00073
Sequence similarities	Belongs to the NusB family.
Sequence caution	The sequence AAK46918.1 differs from that shown. Reason: Frameshift at position 144.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Transcription termination
Ligand	RNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: MTBBASE regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription termination, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein homodimerization activity Inferred from physical interaction Ref.4. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 156

156

N utilization substance protein B homolog HAMAP MF_00073

PRO_0000176557

Secondary structure

1

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156

Helix Strand Turn

Details...

Helix

10 – 27

18

Helix

31 – 44

14

Helix

53 – 64

12

Helix

66 – 74

9

Helix

82 – 84

3

Helix

87 – 102

16

Helix

108 – 122

15

Helix

127 – 138

12

Sequences

	Sequence		Length	Mass (Da)
	P95020 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 84923E642CA5FD15	FASTA	156	16,740
	10 20 30 40 50 60 MSDRKPVRGR HQARKRAVAL LFEAEVRGIS AAEVVDTRAA LAEAKPDIAR LHPYTAAVAR 70 80 90 100 110 120 GVSEHAAHID DLITAHLRGW TLDRLPAVDR AILRVSVWEL LHAADVPEPV VVDEAVQLAK 130 140 150 ELSTDDSPGF VNGVLGQVML VTPQLRAAAQ AVRGGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
[4]	"The crystal structure of NusB from Mycobacterium tuberculosis." Gopal B., Haire L.F., Cox R.A., Colston M.J., Major S., Brannigan J.A., Smerdon S.J., Dodson G. Nat. Struct. Biol. 7:475-478(2000) [PubMed: 10881194] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BX842580 Genomic DNA. Translation: CAB06175.1.
AE000516 Genomic DNA. Translation: AAK46918.1. Frameshift.

PIR

A70658.

RefSeq

NP_217049.1. NC_000962.2.
NP_337104.1. NC_002755.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EYV	X-ray	1.60	A/B	1-156	[»]

ProteinModelPortal

P95020.

SMR

P95020. Positions 7-139.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBMYCT00000003053; EBMYCP00000003053; EBMYCG00000003051.
EBMYCT00000068965; EBMYCP00000067024; EBMYCG00000068960.

GeneID

887359.
925708.

GenomeReviews

Gene locus MT2608 in contig AE000516_GR.
Gene locus Rv2533c in contig AL123456_GR.

KEGG

mtc:MT2608.
mtu:Rv2533c.

PATRIC

18127490. VBIMycTub22151_2848.

TIGR

MT2608.

Organism-specific databases

TubercuList

Rv2533c.

Phylogenomic databases

GeneTree

EBGT00050000017513.

HOGENOM

HBG734409.

OMA

DDSPSFV.

PhylomeDB

P95020.

ProtClustDB

PRK00202.

Family and domain databases

HAMAP

MF_00073. NusB.
[Tree]

InterPro

IPR011605. NusB_fam.
IPR006027. NusB_RsmB_TIM44.
[Graphical view]

Gene3D

G3DSA:1.10.940.10. NusB_RsmB_TIM44. 1 hit.

KO

K03625.

PANTHER

PTHR11078. PTHR11078. 1 hit.

Pfam

PF01029. NusB. 1 hit.
[Graphical view]

SUPFAM

SSF48013. NusB_RsmB_TIM44. 1 hit.

TIGRFAMs

TIGR01951. NusB. 1 hit.

ProtoNet

Search...

Entry information

Entry name

NUSB_MYCTU

Accession

Primary (citable) accession number: P95020

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1997
Last modified:	January 25, 2012
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program