P95020 (NUSB_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N utilization substance protein B homolog
Short name=Protein NusB

Gene names

Name:	nusB
Ordered Locus Names:	Rv2533c, MT2608
ORF Names:	MTCY159.23

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the transcription termination process By similarity. HAMAP-Rule MF_00073
Subunit structure	Homodimer.
Miscellaneous	Was identified as a high-confidence drug target. HAMAP-Rule MF_00073
Sequence similarities	Belongs to the NusB family.
Sequence caution	The sequence AAK46918.1 differs from that shown. Reason: Frameshift at position 144.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Transcription termination
Ligand	RNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, termination Inferred from electronic annotation. Source: HAMAP growth Inferred from mutant phenotype PubMed 12657046. Source: MTBBASE regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 156

156

N utilization substance protein B homolog HAMAP-Rule MF_00073

PRO_0000176557

Secondary structure

1

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156

Helix Strand Turn

Details...

Helix

10 – 27

18

Helix

31 – 44

14

Helix

53 – 64

12

Helix

66 – 74

9

Helix

82 – 84

3

Helix

87 – 102

16

Helix

108 – 122

15

Helix

127 – 138

12

Sequences

	Sequence		Length	Mass (Da)
	P95020 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 84923E642CA5FD15	FASTA	156	16,740
	10 20 30 40 50 60 MSDRKPVRGR HQARKRAVAL LFEAEVRGIS AAEVVDTRAA LAEAKPDIAR LHPYTAAVAR 70 80 90 100 110 120 GVSEHAAHID DLITAHLRGW TLDRLPAVDR AILRVSVWEL LHAADVPEPV VVDEAVQLAK 130 140 150 ELSTDDSPGF VNGVLGQVML VTPQLRAAAQ AVRGGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
[4]	"The crystal structure of NusB from Mycobacterium tuberculosis." Gopal B., Haire L.F., Cox R.A., Colston M.J., Major S., Brannigan J.A., Smerdon S.J., Dodson G. Nat. Struct. Biol. 7:475-478(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BX842580 Genomic DNA. Translation: CAB06175.1.
AE000516 Genomic DNA. Translation: AAK46918.1. Frameshift.
AL123456 Genomic DNA. Translation: CCP45328.1.

PIR

A70658.

RefSeq

NP_217049.1. NC_000962.3.
NP_337104.1. NC_002755.2.
YP_006515974.1. NC_018143.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EYV	X-ray	1.60	A/B	1-156	[»]

ProteinModelPortal

P95020.

SMR

P95020. Positions 7-139.

ModBase

Search...

Protein-protein interaction databases

STRING

83332.Rv2533c.

Proteomic databases

PRIDE

P95020.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAK46918; AAK46918; MT2608.

GeneID

13319252.
887359.
925708.

KEGG

mtc:MT2608.
mtu:Rv2533c.
mtv:RVBD_2533c.

PATRIC

18127490. VBIMycTub22151_2848.

Organism-specific databases

TubercuList

Rv2533c.

Phylogenomic databases

eggNOG

COG0781.

HOGENOM

HOG000281867.

KO

K03625.

OMA

WELLYND.

ProtClustDB

PRK00202.

Family and domain databases

Gene3D

1.10.940.10. 1 hit.

HAMAP

MF_00073. NusB.

InterPro

IPR011605. NusB_fam.
IPR006027. NusB_RsmB_TIM44.
[Graphical view]

PANTHER

PTHR11078. PTHR11078. 1 hit.

Pfam

PF01029. NusB. 1 hit.
[Graphical view]

SUPFAM

SSF48013. NusB_RsmB_TIM44. 1 hit.

TIGRFAMs

TIGR01951. nusB. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P95020.

Entry information

Entry name

NUSB_MYCTU

Accession

Primary (citable) accession number: P95020
Secondary accession number(s): L0TCN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program