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Reviewed, UniProtKB/Swiss-Prot P94974 (SYK2_MYCTU)

Last modified November 3, 2009. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Putative lysyl-tRNA synthetase 2
    EC=6.1.1.6
Alternative name(s):
    Lysine--tRNA ligase 2
      Short name=LysRS 2
Gene names
Name: lysS2
Synonyms: lysX
Ordered Locus Names: Rv1640c, MT1678
ORF Names: MTCY06H11.04c
OrganismMycobacterium tuberculosis [Complete proteome] [HAMAP]
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

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Protein attributes

Sequence length1172 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00252

Cofactor

Binds 3 magnesium ions per subunit By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Potential.

Miscellaneous

There are two lysyl-tRNA ligases in M.tuberculosis. HAMAP MF_00252

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11721172Putative lysyl-tRNA synthetase 2 HAMAP MF_00252
PRO_0000152657

Regions

Transmembrane80 – 10021 Potential
Transmembrane122 – 14221 Potential
Transmembrane146 – 16621 Potential
Transmembrane177 – 19721 Potential
Transmembrane214 – 23421 Potential
Transmembrane272 – 29221 Potential
Region1 – 663663Unknown HAMAP MF_00252
Region664 – 1172509Lysyl-tRNA synthetase HAMAP MF_00252

Sites

Metal binding10841Magnesium 1 By similarity
Metal binding10911Magnesium 1 By similarity
Metal binding10911Magnesium 2 By similarity

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Sequences

Sequence LengthMass (Da)Tools
P94974-1 [UniParc].

Last modified July 1, 1997. Version 2.
Checksum: 97E91546A1ACCAEB

FASTA1,172128,240
        10         20         30         40         50         60 
MGLHLTVPGL RRDGRGVQSN SHDTSSKTTA DISRCPQHTD AGLQRAATPG ISRLLGISSR 

        70         80         90        100        110        120 
SVTLTKPRSA TRGNSRYHWV PAAAGWTVGV IATLSLLASV SPLIRWIIKV PREFINDYLF 

       130        140        150        160        170        180 
NFPDTNFAWS FVLALLAAAL TARKRIAWLV LLANMVLAAV VNAAEIAAGG NTAAESFGEN 

       190        200        210        220        230        240 
LGFAVHVVAI VVLVLGYREF WAKVRRGALF RAAAVWLAGA VVGIVASWGL VELFPGSLAP 

       250        260        270        280        290        300 
DERLGYAANR VVGFALADPD LFTGRPHVFL NAIFGLFGAF ALIGAAIVLF LSQRADNALT 

       310        320        330        340        350        360 
GEDESAIRGL LDLYGKDDSL GYFATRRDKS VVFASSGRAC ITYRVEVGVC LASGDPVGDH 

       370        380        390        400        410        420 
RAWPQAVDAW LRLCQTYGWA PGVMGASSQG AQTYREAGLT ALELGDEAIL RPADFKLSGP 

       430        440        450        460        470        480 
EMRGVRQAVT RARRAGLTVR IRRHRDIAED EMAQTITRAD SWRDTETERG FSMALGRLGD 

       490        500        510        520        530        540 
PADSDCLLVE AIDPHNQVLA MLSLVPWGTT GVSLDLMRRS PQSPNGTIEL MVSELALHAE 

       550        560        570        580        590        600 
SLGITRISLN FAVFRAAFEQ GAQLGAGPVA RLWRGLLVFF SRWWQLETLY RSNMKYQPEW 

       610        620        630        640        650        660 
VPRYACYEDA RVIPRVGVAS VIAEGFLVLP FSRRNRVHTG HHPAVPERLA ATGLLHHDGS 

       670        680        690        700        710        720 
APDVSGLRQV GLTNGDGVER RLPEQVRVRF DKLEKLRSSG IDAFPVGRPP SHTVAQALAA 

       730        740        750        760        770        780 
DHQASVSVSG RIMRIRNYGG VLFAQLRDWS GEMQVLLDNS RLDQGCAADF NAATDLGDLV 

       790        800        810        820        830        840 
EMTGHMGASK TGTPSLIVSG WRLIGKCLRP LPNKWKGLLD PEARVRTRYL DLAVNAESRA 

       850        860        870        880        890        900 
LITARSSVLR AVRETLFAKG FVEVETPILQ QLHGGATARP FVTHINTYSM DLFLRIAPEL 

       910        920        930        940        950        960 
YLKRLCVGGV ERVFELGRAF RNEGVDFSHN PEFTLLEAYQ AHADYLEWID GCRELIQNAA 

       970        980        990       1000       1010       1020 
QAANGAPIAM RPRTDKGSDG TRHHLEPVDI SGIWPVRTVH DAISEALGER IDADTGLTTL 

      1030       1040       1050       1060       1070       1080 
RKLCDAAGVP YRTQWDAGAV VLELYEHLVE CRTEQPTFYI DFPTSVSPLT RPHRSKRGVA 

      1090       1100       1110       1120       1130       1140 
ERWDLVAWGI ELGTAYSELT DPVEQRRRLQ EQSLLAAGGD PEAMELDEDF LQAMEYAMPP 

      1150       1160       1170 
TGGLGMGIDR VVMLITGRSI RETLPFPLAK PH

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Cross-references

Sequence databases

BX842577 Genomic DNA. Translation: CAB06635.1.
AE000516 Genomic DNA. Translation: AAK45947.1. Different initiation.
PIRC70619.
RefSeqNP_216156.1.
NP_336133.1.

3D structure databases

HSSPHSSP built from PDB template 1BBW based on UniProtKB P13030.
ModBaseSearch...

Genome annotation databases

GeneID885428.
923983.
GenomeReviewsGene locus MT1678 in contig AE000516_GR.
Gene locus Rv1640c in contig AL123456_GR.
KEGGmtc:MT1678.
mtu:Rv1640c.
TIGRMT1678.

Organism-specific databases

TubercuListRv1640c.

Phylogenomic databases

HOGENOMP94974.
OMAATRRDKA.

Enzyme and pathway databases

BRENDA6.1.1.6. 809.

Family and domain databases

HAMAPMF_00252. Fused.
[Tree]
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II_cons-reg.
IPR007424. DUF470.
IPR007425. DUF471.
IPR007426. DUF472.
IPR002313. Lys-tRNA-synth_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA_bd_OB_tRNA-helicase.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHERPTHR22594. aa-tRNA-synt_II. 1 hit.
PTHR22594:SF4. tRNA-synt_lys_2. 1 hit.
PfamPF04329. DUF470. 1 hit.
PF04330. DUF471. 1 hit.
PF04331. DUF472. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti. 1 hit.
[Graphical view]
PRINTSPR00982. TRNASYNTHLYS.
TIGRFAMsTIGR00499. lysS_bact. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameSYK2_MYCTU
AccessionPrimary (citable) accession number: P94974
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 1, 1997
Last modified: November 3, 2009
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents