P94967 (ALR_MYCSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Synonyms:	alrA

Organism

Mycobacterium smegmatis

Taxonomic identifier

1772 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis. Overexpression due to a natural mutation in the promoter is responsible for mediating resistance to D-cycloserine (DCS) in mycobacteria. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Alanine racemase HAMAP MF_01201		PRO_0000114538
Sites
	Active site	46	1	Proton acceptor; specific for D-alanine By similarity
	Active site	275	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	46	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P94967 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: A53F326029904830	FASTA	389	41,056
	10 20 30 40 50 60 MQTTEPMTPP APLASAQTVI DLGAIDHNVR VLRELAGSAD VMAVVKADAY GHGALPVART 70 80 90 100 110 120 ALAAGAAALG VATIPEALAL REGGITAPVL AWLHPPGTDF APAIAADVEV AVSSRRQLEQ 130 140 150 160 170 180 VTAAAAEVGR TATVTVKVDT GLSRNGVGAA DYPEVLDVLR RAQADGAIRV RGLMSHLVHG 190 200 210 220 230 240 DDPENPFNGL QGQRLADMRV YAREHGVDYE VAHLCNSPAA MTRPDLAFEM VRPGISLYGL 250 260 270 280 290 300 SPIPERGDMG LRPAMTLKCP VALVRSVHAG DGVSYGHRWV ADRDTTLGLL PIGYADGVYR 310 320 330 340 350 360 ALSGRIDVLI KGRRRRAVGR ICMDQFVVDL GPDADDVAVG DDAILFGPGA NGEPTAQDWA 370 380 ELLDTIHYEV VTSPRGRVTR TYLPAGQQD

References

[1]	"Overexpression of the D-alanine racemase gene confers resistance to D-cycloserine in Mycobacterium smegmatis." Caceres N.E., Harris N.B., Wellehan J.F., Feng Z., Kapur V., Barletta R.G. J. Bacteriol. 179:5046-5055(1997) [PubMed: 9260945] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U70872 Genomic DNA. Translation: AAC45589.1.
3D structure databases
ProteinModelPortal	P94967.
SMR	P94967. Positions 16-385.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_MYCSM

Accession

Primary (citable) accession number: P94967

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program