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P94966

- BIOB_METSK

UniProt

P94966 - BIOB_METSK

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Protein
Biotin synthase
Gene
bioB
Organism
Methylobacillus sp. (strain KT1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi75 – 751Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi82 – 821Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi118 – 1181Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi147 – 1471Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi207 – 2071Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi279 – 2791Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
OrganismiMethylobacillus sp. (strain KT1)
Taxonomic identifieri79675 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Biotin synthaseUniRule annotation
PRO_0000185557Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP94966.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

P94966-1 [UniParc]FASTAAdd to Basket

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MRDSVIDIRG LDRVQRARVQ AKEEAPKRWS VDDIVALFEL PFSDLMHRAQ    50
SVHRENFDPN GVQVSTLLSI KTGGCSEDCG YCPQAARYHT DVEKQDLHAA 100
GRGTRSRVLP RRMRQPFCMA AWQPKQRDLE PVLAMIREVK AMGLETCATL 150
GMLKDGQAEQ LKEAGLDYYN HNLDTAPEYY GEVITTRTYQ DRLDTLDRVR 200
EQDINVCCGG IIGMGESRVQ RAGLLAQLAN MERPPESVPD QLLTQVEGTP 250
MYGMDELDPF EFVRTIAAAR ITMPQSFVRL SVGRQSMHEG IQALCFLAGA 300
NSIFYGEKLL TTGNPEAEAD RKLFDKLGIH PL 332
Length:332
Mass (Da):37,274
Last modified:July 28, 2009 - v2
Checksum:iFFF153BAD2AF2981
GO

Sequence cautioni

The sequence AAC44582.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U31280 Genomic DNA. Translation: AAC44582.1. Different initiation.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U31280 Genomic DNA. Translation: AAC44582.1 . Different initiation.

3D structure databases

ProteinModelPortali P94966.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Two new members of the bio B superfamily: cloning, sequencing and expression of bio B genes of Methylobacillus flagellatum and Corynebacterium glutamicum."
    Serebriiskii I.G., Vassin V.M., Tsygankov Y.D.
    Gene 175:15-22(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiBIOB_METSK
AccessioniPrimary (citable) accession number: P94966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 28, 2009
Last modified: February 19, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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