P94954 (MCH_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methenyltetrahydromethanopterin cyclohydrolase
EC=3.5.4.27

Alternative name(s):
Methenyl-H4MPT cyclohydrolase

Gene names

Name:	mch
Ordered Locus Names:	MK0625

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reversible interconversion of 5-formyl-H4MPT to methenyl-H4MPT+. HAMAP MF_00486
Catalytic activity	5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = 5-formyl-5,6,7,8-tetrahydromethanopterin. HAMAP MF_00486
Pathway	One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 3/3. HAMAP MF_00486
Subunit structure	Homotrimer.
Subcellular location	Cytoplasm HAMAP MF_00486.
Sequence similarities	Belongs to the MCH family.
Sequence caution	The sequence AAM01840.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA70072.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methenyltetrahydromethanopterin cyclohydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed HAMAP MF_00486

Chain

2 – 316

315

Methenyltetrahydromethanopterin cyclohydrolase HAMAP MF_00486

PRO_0000140882

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

316

Helix Strand Turn

Details...

Helix

4 – 17

14

Helix

19 – 22

4

Beta strand

25 – 28

4

Beta strand

34 – 42

9

Helix

46 – 56

11

Turn

57 – 59

3

Beta strand

61 – 70

10

Beta strand

73 – 84

12

Helix

85 – 89

5

Turn

90 – 93

4

Beta strand

97 – 101

5

Beta strand

104 – 110

7

Helix

113 – 116

4

Helix

120 – 126

7

Beta strand

135 – 140

6

Helix

147 – 157

11

Helix

161 – 163

3

Beta strand

164 – 169

6

Beta strand

171 – 173

3

Helix

174 – 181

8

Helix

184 – 194

11

Helix

200 – 202

3

Beta strand

203 – 211

9

Helix

219 – 233

15

Beta strand

235 – 240

6

Helix

246 – 252

7

Helix

255 – 257

3

Turn

259 – 262

4

Helix

265 – 271

7

Turn

272 – 274

3

Helix

276 – 278

3

Helix

281 – 283

3

Beta strand

287 – 293

7

Turn

294 – 296

3

Beta strand

299 – 303

5

Helix

307 – 313

7

Sequences

	Sequence		Length	Mass (Da)
	P94954 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: D6D4097DE6BE0500	FASTA	316	34,044
	10 20 30 40 50 60 MVSVNENALP LVERMIERAE LLNVEVQELE NGTTVIDCGV EAAGGFEAGL LFSEVCMGGL 70 80 90 100 110 120 ATVELTEFEH DGLCLPAVQV TTDHPAVSTL AAQKAGWQVQ VGDYFAMGSG PARALALKPK 130 140 150 160 170 180 ETYEEIDYED DADVAILCLE SSELPDEDVA EHVADECGVD PENLYLLVAP TASIVGSVQV 190 200 210 220 230 240 SARVVETGLY KLLEVLEYDV TRVKYATGTA PIAPVADDDG EAMGRTNDCI LYGGTVYLYV 250 260 270 280 290 300 EGDDELPEVV EELPSEASED YGKPFMKIFE EADYDFYKID PGVFAPARVV VNDLSTGKTY 310 TAGEINVDVL KESFGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Overproduction and one-step purification of the N5,N10-methenyltetrahydromethanopterin cyclohydrolase (Mch) from the hyperthermophilic Methanopyrus kandleri." Vaupel M., Vorholt J.A., Thauer R.K. Extremophiles 2:15-22(1998) [PubMed: 9676239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.
[2]	"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.
[3]	"The crystal structure of methenyltetrahydromethanopterin cyclohydrolase from the hyperthermophilic archaeon Methanopyrus kandleri." Grabarse W., Vaupel M., Vorholt J.A., Shima S., Thauer R.K., Wittershagen A., Bourenkov G., Bartunik H.D., Ermler U. Structure 7:1257-1268(1999) [PubMed: 10545331] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y08849 Genomic DNA. Translation: CAA70072.1. Different initiation.
AE009439 Genomic DNA. Translation: AAM01840.1. Different initiation.

PIR

T45099.

RefSeq

NP_613910.1. NC_003551.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QLM	X-ray	2.00	A	1-314	[»]

ProteinModelPortal

P94954.

SMR

P94954. Positions 1-316.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1476726.

GenomeReviews

Gene locus MK0625 in contig AE009439_GR.

KEGG

mka:MK0625.

NMPDR

fig|190192.1.peg.623.

Phylogenomic databases

HOGENOM

HBG497176.

OMA

YFAMGSG.

ProtClustDB

PRK02264.

Enzyme and pathway databases

BioCyc

MKAN190192:MK0625-MONOMER.

Family and domain databases

HAMAP

MF_00486. McH.
[Tree]

InterPro

IPR003209. METHMP_CycHdrlase.
[Graphical view]

KO

K01499.

Pfam

PF02289. MCH. 1 hit.
[Graphical view]

SUPFAM

SSF56199. Cyclohydrolase. 1 hit.

TIGRFAMs

TIGR03120. One_C_mch. 1 hit.

ProtoNet

Search...

Entry information

Entry name

MCH_METKA

Accession

Primary (citable) accession number: P94954

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 88 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program