Reviewed,
UniProtKB/Swiss-Prot P94951 (MTD_METKA)
Last modified
November 25, 2008.
Version 64.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: F420-dependent methylenetetrahydromethanopterin dehydrogenase Short name=MTD EC=1.5.99.9 Alternative name(s): Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase | ||||
| Gene names |
| ||||
| Organism | Methanopyrus kandleri [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 2320 [NCBI] | ||||
| Taxonomic lineage | Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus |
Protein attributes
| Sequence length | 283 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to methylene-H(4)MPT. |
| Catalytic activity | 5,10-methylenetetrahydromethanopterin + coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420. |
| Enzyme regulation | Strictly dependent on the presence of salts for activity. |
| Pathway | |
| Subunit structure | Homohexamer composed of a trimer of dimers. |
| Sequence similarities | Belongs to the MTD family. |
| Biophysicochemical properties | Temperature dependence: Optimum temperature is 75 degrees Celsius. |
Ontologies
Keywords | |
|---|---|
| Biological process | Methanogenesis One-carbon metabolism |
| Molecular function | Oxidoreductase |
| Technical term | 3D-structure Complete proteome Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | methanogenesis Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro methylenetetrahydromethanopterin dehydrogenase activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||||||||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||||||||||||||||||||||||||||||||||||||||
| Chain | 2 – 283 | 282 | F420-dependent methylenetetrahydromethanopterin dehydrogenase | PRO_0000075039 | |||||||||||||||||||||||||||||||||||||||||||
Experimental info | |||||||||||||||||||||||||||||||||||||||||||||||
| Sequence conflict | 11 | 1 | C → E AA sequence Ref.3 | ||||||||||||||||||||||||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 4 – 10 | 7 | |||||||||||||||||||||||||||||||||||||||||||||
| Helix | 15 – 19 | 5 | |||||||||||||||||||||||||||||||||||||||||||||
| Helix | 22 – 24 | 3 | |||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 31 – 39 | 9 | |||||||||||||||||||||||||||||||||||||||||||||
| Helix | 46 – 63 | 18 | |||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 66 – 71 | 6 | |||||||||||||||||||||||||||||||||||||||||||||
| Helix | 78 – 88 | 11 | |||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 90 – 92 | 3 | |||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 94 – 99 | 6 | |||||||||||||||||||||||||||||||||||||||||||||
| Helix | 100 – 105 | 6 | |||||||||||||||||||||||||||||||||||||||||||||
| Helix | 106 – 111 | 6 | |||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 115 – 119 | 5 | |||||||||||||||||||||||||||||||||||||||||||||
| Turn | 129 – 131 | 3 | |||||||||||||||||||||||||||||||||||||||||||||
| Helix | 134 – 150 | 17 | |||||||||||||||||||||||||||||||||||||||||||||
| Helix | 153 – 169 | 17 | |||||||||||||||||||||||||||||||||||||||||||||
| Helix | 175 – 177 | 3 | |||||||||||||||||||||||||||||||||||||||||||||
| Beta strand | 180 – 183 | 4 | |||||||||||||||||||||||||||||||||||||||||||||
| Helix | 185 – 190 | 6 | |||||||||||||||||||||||||||||||||||||||||||||
| Helix | 197 – 222 | 26 | |||||||||||||||||||||||||||||||||||||||||||||
| Helix | 227 – 257 | 31 | |||||||||||||||||||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Overexpression of the coenzyme-F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase gene from the hyperthermophilic Methanopyrus kandleri." Klein A.R., Thauer R.K. Eur. J. Biochem. 245:386-391(1997) [PubMed: 9151968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. |
| [2] | "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938. |
| [3] | "Two N5,N10-methylenetetrahydromethanopterin dehydrogenases in the extreme thermophile Methanopyrus kandleri: characterization of the coenzyme F420-dependent enzyme." Klein A.R., Koch J., Stetter K.O., Thauer R.K. Arch. Microbiol. 160:186-192(1993) [PubMed: 8215796] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-35, CHARACTERIZATION. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938. |
| [4] | "Coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase from Methanopyrus kandleri: the selenomethionine-labelled and non-labelled enzyme crystallized in two different forms." Hagemeier C.H., Shima S., Warkentin E., Thauer R.K., Ermler U. Acta Crystallogr. D 59:1653-1655(2003) [PubMed: 12925803] [Abstract] Cited for: CRYSTALLIZATION. |
| [5] | "Coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase (Mtd) from Methanopyrus kandleri: a methanogenic enzyme with an unusual quarternary structure." Hagemeier C.H., Shima S., Thauer R.K., Bourenkov G., Bartunik H.D., Ermler U. J. Mol. Biol. 332:1047-1057(2003) [PubMed: 14499608] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.54 ANGSTROMS). |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Y10251 Genomic DNA. Translation: CAA71298.1. AE010303 Genomic DNA. Translation: AAM01228.1. | |||||||||||||||||||||||||||||||
| RefSeq | NP_613298.1. | ||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||
| GeneID | 1477313. | ||||||||||||||||||||||||||||||
| GenomeReviews | Gene locus MK0011 in contig AE009439_GR. | ||||||||||||||||||||||||||||||
| KEGG | mka:MK0011. | ||||||||||||||||||||||||||||||
| NMPDR | fig|190192.1.peg.11. | ||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||
| CMR | Search... | ||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||
| HOGENOM | P94951. | ||||||||||||||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||||||||||||||
| BioCyc | MKAN190192:MK0011-MON. | ||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||
| HAMAP | MF_00058. [Tree] | ||||||||||||||||||||||||||||||
| InterPro | IPR002844. Methylene_DHase. [Graphical view] | ||||||||||||||||||||||||||||||
| Pfam | PF01993. MTD. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||
| PIRSF | PIRSF005627. MTD. 1 hit. | ||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||
Entry information
| Entry name | MTD_METKA | ||||||||
| Accession | Primary (citable) accession number: P94951 Secondary accession number(s): Q9UWL4 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


