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Reviewed, UniProtKB/Swiss-Prot P94951 (MTD_METKA)

Last modified January 19, 2010. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    F420-dependent methylenetetrahydromethanopterin dehydrogenase
      Short name=MTD
    EC=1.5.99.9
Alternative name(s):
    Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase
Gene names
Name: mtd
Ordered Locus Names: MK0011
OrganismMethanopyrus kandleri [Complete proteome] [HAMAP]
Taxonomic identifier2320 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the reversible reduction of methenyl-H4MPT+ to methylene-H4MPT. Ref.1

Catalytic activity

5,10-methylenetetrahydromethanopterin + coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_00058

Enzyme regulation

Strictly dependent on the presence of salts for activity. HAMAP MF_00058

Pathway

One-carbon metabolism; methanogenesis from CO(2); 5,10-methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-tetrahydromethanopterin (coenzyme F420 route): step 1/1. HAMAP MF_00058

Subunit structure

Homohexamer composed of a trimer of dimers. HAMAP MF_00058

Sequence similarities

Belongs to the MTD family.

Biophysicochemical properties

Temperature dependence:

Optimum temperature is 75 degrees Celsius. HAMAP MF_00058

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 283282F420-dependent methylenetetrahydromethanopterin dehydrogenase HAMAP MF_00058
PRO_0000075039

Experimental info

Sequence conflict111C → E AA sequence Ref.3

Secondary structure

....................................... 283
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P94951-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: D2B778CB34D3034C

FASTA28331,382
        10         20         30         40         50         60 
MTVAKAIFIK CGNLGTSMMM DMLLDERADR EDVEFRVVGT SVKMDPECVE AAVEMALDIA 

        70         80         90        100        110        120 
EDFEPDFIVY GGPNPAAPGP SKAREMLADS EYPAVIIGDA PGLKVKDEME EQGLGYILVK 

       130        140        150        160        170        180 
PDAMLGARRE FLDPVEMAIY NADLMKVLAA TGVFRVVQEA FDELIEKAKE DEISENDLPK 

       190        200        210        220        230        240 
LVIDRNTLLE REEFENPYAM VKAMAALEIA ENVADVSVEG CFVEQDKERY VPIVASAHEM 

       250        260        270        280 
MRKAAELADE ARELEKSNDA VLRTPHAPDG KVLSKRKFME DPE 

« Hide

References

« Hide 'large scale' references
[1]"Overexpression of the coenzyme-F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase gene from the hyperthermophilic Methanopyrus kandleri."
Klein A.R., Thauer R.K.
Eur. J. Biochem. 245:386-391(1997) [PubMed: 9151968] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens."
Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A.
Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.
[3]"Two N5,N10-methylenetetrahydromethanopterin dehydrogenases in the extreme thermophile Methanopyrus kandleri: characterization of the coenzyme F420-dependent enzyme."
Klein A.R., Koch J., Stetter K.O., Thauer R.K.
Arch. Microbiol. 160:186-192(1993) [PubMed: 8215796] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-35, CHARACTERIZATION.
Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.
[4]"Coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase from Methanopyrus kandleri: the selenomethionine-labelled and non-labelled enzyme crystallized in two different forms."
Hagemeier C.H., Shima S., Warkentin E., Thauer R.K., Ermler U.
Acta Crystallogr. D 59:1653-1655(2003) [PubMed: 12925803] [Abstract]
Cited for: CRYSTALLIZATION.
[5]"Coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase (Mtd) from Methanopyrus kandleri: a methanogenic enzyme with an unusual quarternary structure."
Hagemeier C.H., Shima S., Thauer R.K., Bourenkov G., Bartunik H.D., Ermler U.
J. Mol. Biol. 332:1047-1057(2003) [PubMed: 14499608] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.54 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y10251 Genomic DNA. Translation: CAA71298.1.
AE009439 Genomic DNA. Translation: AAM01228.1.
RefSeqNP_613298.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1QV9X-ray1.54A/B/C1-283[»]
1U6IX-ray2.20A/B/C/D/E/F/G/H/I/J/K/L2-282[»]
1U6JX-ray2.40A/B/C/D/E/F/G/H/I/J/K/L2-282[»]
1U6KX-ray1.55A/B/C2-282[»]
3IQEX-ray1.80A/B/C/D/E/F1-283[»]
3IQFX-ray2.10A/B/C/D/E/F/G/H/I/J/K/L1-283[»]
3IQZX-ray2.10A/B/C/D/E/F1-283[»]
ModBaseSearch...

Genome annotation databases

GeneID1477313.
GenomeReviewsGene locus MK0011 in contig AE009439_GR.
KEGGmka:MK0011.
NMPDRfig|190192.1.peg.11.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG538567.
OMAIVASAHE.

Enzyme and pathway databases

BioCycMKAN190192:MK0011-MONOMER.
BRENDA1.5.99.9. 7577.

Family and domain databases

HAMAPMF_00058. MTD.
[Tree]
InterProIPR002844. Methylene_DH.
[Graphical view]
PfamPF01993. MTD. 1 hit.
[Graphical view]
PIRSFPIRSF005627. MTD. 1 hit.
ProDomPD017604. Methylene_DH. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameMTD_METKA
AccessionPrimary (citable) accession number: P94951
Secondary accession number(s): Q9UWL4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: January 19, 2010
This is version 74 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents