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P94846

- UVRB_HELPY

UniProt

P94846 - UVRB_HELPY

Protein

UvrABC system protein B

Gene

uvrB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi38 – 458ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. DNA binding Source: UniProtKB-HAMAP
    3. excinuclease ABC activity Source: UniProtKB-HAMAP
    4. helicase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nucleotide-excision repair Source: UniProtKB-HAMAP
    2. SOS response Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Excision nuclease

    Keywords - Biological processi

    DNA damage, DNA excision, DNA repair, SOS response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciHPY:HP1114-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UvrABC system protein BUniRule annotation
    Short name:
    Protein UvrBUniRule annotation
    Alternative name(s):
    Excinuclease ABC subunit BUniRule annotation
    Gene namesi
    Name:uvrBUniRule annotation
    Ordered Locus Names:HP_1114
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000429: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 658658UvrABC system protein BPRO_0000138396Add
    BLAST

    Proteomic databases

    PRIDEiP94846.

    Interactioni

    Subunit structurei

    Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

    Protein-protein interaction databases

    DIPiDIP-3238N.
    IntActiP94846. 18 interactions.
    MINTiMINT-171452.
    STRINGi85962.HP1114.

    Structurei

    3D structure databases

    ProteinModelPortaliP94846.
    SMRiP94846. Positions 3-597.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 414390Helicase ATP-bindingUniRule annotationAdd
    BLAST
    Domaini433 – 607175Helicase C-terminalUniRule annotationAdd
    BLAST
    Domaini623 – 65836UVRUniRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi91 – 11424Beta-hairpinAdd
    BLAST

    Domaini

    The beta-hairpin motif is involved in DNA binding.UniRule annotation

    Sequence similaritiesi

    Belongs to the UvrB family.UniRule annotation
    Contains 1 helicase ATP-binding domain.UniRule annotation
    Contains 1 helicase C-terminal domain.UniRule annotation
    Contains 1 UVR domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0556.
    KOiK03702.
    OMAiESGNPLH.
    OrthoDBiEOG6B360R.

    Family and domain databases

    Gene3Di3.40.50.300. 4 hits.
    4.10.860.10. 1 hit.
    HAMAPiMF_00204. UvrB.
    InterProiIPR006935. Helicase/UvrB_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR001943. UVR_dom.
    IPR004807. UvrB.
    IPR024759. UvrB_YAD/RRR_dom.
    [Graphical view]
    PANTHERiPTHR24029. PTHR24029. 1 hit.
    PfamiPF00271. Helicase_C. 1 hit.
    PF04851. ResIII. 1 hit.
    PF02151. UVR. 1 hit.
    PF12344. UvrB. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF46600. SSF46600. 1 hit.
    SSF52540. SSF52540. 3 hits.
    TIGRFAMsiTIGR00631. uvrb. 1 hit.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 2 hits.
    PS51194. HELICASE_CTER. 1 hit.
    PS50151. UVR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P94846-1 [UniParc]FASTAAdd to Basket

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    MPLFDLKSPY PPAGDQPQAI EALTKSLKNN NHYQTLVGVT GSGKTYTMAN    50
    IIAQTNKPAL IMSHNKTLCA QLYSEFKAFF PHNRVEYFIS HFDYYQPESY 100
    IPRRDLFIEK DSSINDDLER LRLSATTSLL GYDDVIVIAS VSANYGLGNP 150
    EEYLKVMEKI KVGEKRAYKS FLLKLVEMGY SRNEVVFDRG SFRATGECVD 200
    IFPAYNDAEF IRIEFFGDEI ERIAVFDALE KNEIKRLDSV MLYAASQFAV 250
    GSERLNLAIK SIEDELALRL KFFKEQDKML EYNRLKQRTE HDLEMISATG 300
    VCKGIENYAR HFTGKAPNET PFCLFDYLGI FEREFLVIVD ESHVSLPQFG 350
    GMYAGDMSRK SVLVEYGFRL PSALDNRPLK FDEFIHKNCQ FLFVSATPNK 400
    LELELSKKNV AEQIIRPTGL LDPKFEVRDS DKQVQDLFDE IKLVVARGER 450
    VLITTLTKKM AEELCKYYAE WGLKARYMHS EIDAIERNHI IRSLRLKEFD 500
    ILIGINLLRE GLDLPEVSLV AIMDADKEGF LRSETSLIQT MGRAARNANG 550
    KVLLYAKKIT QSMQKAFEIT SYRRAKQEEF NKIHNITPKT VTRALEEELK 600
    LRDDEIRIAK ALKKDKMPKS EREKIIKELD KKMRECTKNL DFEEAMRLRD 650
    EIAQLRTL 658
    Length:658
    Mass (Da):75,916
    Last modified:November 1, 1997 - v2
    Checksum:i06BC0EE689AB3ADE
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti59 – 591A → T in strain: 84-183.
    Natural varianti231 – 2311K → R in strain: 84-183.
    Natural varianti259 – 2591I → V in strain: 84-183.
    Natural varianti376 – 3761N → T in strain: 84-183.
    Natural varianti407 – 4071K → Q in strain: 84-183.
    Natural varianti443 – 4431L → S in strain: 84-183.
    Natural varianti475 – 4751A → V in strain: 84-183.
    Natural varianti501 – 5011I → V in strain: 84-183.
    Natural varianti607 – 6071R → K in strain: 84-183.
    Natural varianti636 – 6372CT → HA in strain: 84-183.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U80807 Genomic DNA. Translation: AAC46270.1.
    AE000511 Genomic DNA. Translation: AAD08158.1.
    PIRiB64659.
    RefSeqiNP_207905.1. NC_000915.1.
    YP_006935029.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD08158; AAD08158; HP_1114.
    GeneIDi13870307.
    899650.
    KEGGiheo:C694_05745.
    hpy:HP1114.
    PATRICi20593569. VBIHelPyl33062_1164.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U80807 Genomic DNA. Translation: AAC46270.1 .
    AE000511 Genomic DNA. Translation: AAD08158.1 .
    PIRi B64659.
    RefSeqi NP_207905.1. NC_000915.1.
    YP_006935029.1. NC_018939.1.

    3D structure databases

    ProteinModelPortali P94846.
    SMRi P94846. Positions 3-597.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-3238N.
    IntActi P94846. 18 interactions.
    MINTi MINT-171452.
    STRINGi 85962.HP1114.

    Proteomic databases

    PRIDEi P94846.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD08158 ; AAD08158 ; HP_1114 .
    GeneIDi 13870307.
    899650.
    KEGGi heo:C694_05745.
    hpy:HP1114.
    PATRICi 20593569. VBIHelPyl33062_1164.

    Phylogenomic databases

    eggNOGi COG0556.
    KOi K03702.
    OMAi ESGNPLH.
    OrthoDBi EOG6B360R.

    Enzyme and pathway databases

    BioCyci HPY:HP1114-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.300. 4 hits.
    4.10.860.10. 1 hit.
    HAMAPi MF_00204. UvrB.
    InterProi IPR006935. Helicase/UvrB_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR001943. UVR_dom.
    IPR004807. UvrB.
    IPR024759. UvrB_YAD/RRR_dom.
    [Graphical view ]
    PANTHERi PTHR24029. PTHR24029. 1 hit.
    Pfami PF00271. Helicase_C. 1 hit.
    PF04851. ResIII. 1 hit.
    PF02151. UVR. 1 hit.
    PF12344. UvrB. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46600. SSF46600. 1 hit.
    SSF52540. SSF52540. 3 hits.
    TIGRFAMsi TIGR00631. uvrb. 1 hit.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 2 hits.
    PS51194. HELICASE_CTER. 1 hit.
    PS50151. UVR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the Helicobacter pylori uvr B gene."
      Thompson S.A., Latch R.L., Blaser J.M.
      Gene 209:113-122(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 53726 / 84-183.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700392 / 26695.

    Entry informationi

    Entry nameiUVRB_HELPY
    AccessioniPrimary (citable) accession number: P94846
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3