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Protein

UvrABC system protein B

Gene

uvrB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 458ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. excinuclease ABC activity Source: UniProtKB-HAMAP
  4. helicase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nucleotide-excision repair Source: UniProtKB-HAMAP
  2. SOS response Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Excision nuclease

Keywords - Biological processi

DNA damage, DNA excision, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHPY:HP1114-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:HP_1114
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 658658UvrABC system protein BPRO_0000138396Add
BLAST

Proteomic databases

PRIDEiP94846.

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Protein-protein interaction databases

DIPiDIP-3238N.
IntActiP94846. 18 interactions.
MINTiMINT-171452.
STRINGi85962.HP1114.

Structurei

3D structure databases

ProteinModelPortaliP94846.
SMRiP94846. Positions 3-597.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 414390Helicase ATP-bindingUniRule annotationAdd
BLAST
Domaini433 – 607175Helicase C-terminalUniRule annotationAdd
BLAST
Domaini623 – 65836UVRUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi91 – 11424Beta-hairpinAdd
BLAST

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation
Contains 1 helicase ATP-binding domain.UniRule annotation
Contains 1 helicase C-terminal domain.UniRule annotation
Contains 1 UVR domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0556.
KOiK03702.
OMAiNKTLCAQ.
OrthoDBiEOG6B360R.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB.
InterProiIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P94846-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLFDLKSPY PPAGDQPQAI EALTKSLKNN NHYQTLVGVT GSGKTYTMAN
60 70 80 90 100
IIAQTNKPAL IMSHNKTLCA QLYSEFKAFF PHNRVEYFIS HFDYYQPESY
110 120 130 140 150
IPRRDLFIEK DSSINDDLER LRLSATTSLL GYDDVIVIAS VSANYGLGNP
160 170 180 190 200
EEYLKVMEKI KVGEKRAYKS FLLKLVEMGY SRNEVVFDRG SFRATGECVD
210 220 230 240 250
IFPAYNDAEF IRIEFFGDEI ERIAVFDALE KNEIKRLDSV MLYAASQFAV
260 270 280 290 300
GSERLNLAIK SIEDELALRL KFFKEQDKML EYNRLKQRTE HDLEMISATG
310 320 330 340 350
VCKGIENYAR HFTGKAPNET PFCLFDYLGI FEREFLVIVD ESHVSLPQFG
360 370 380 390 400
GMYAGDMSRK SVLVEYGFRL PSALDNRPLK FDEFIHKNCQ FLFVSATPNK
410 420 430 440 450
LELELSKKNV AEQIIRPTGL LDPKFEVRDS DKQVQDLFDE IKLVVARGER
460 470 480 490 500
VLITTLTKKM AEELCKYYAE WGLKARYMHS EIDAIERNHI IRSLRLKEFD
510 520 530 540 550
ILIGINLLRE GLDLPEVSLV AIMDADKEGF LRSETSLIQT MGRAARNANG
560 570 580 590 600
KVLLYAKKIT QSMQKAFEIT SYRRAKQEEF NKIHNITPKT VTRALEEELK
610 620 630 640 650
LRDDEIRIAK ALKKDKMPKS EREKIIKELD KKMRECTKNL DFEEAMRLRD

EIAQLRTL
Length:658
Mass (Da):75,916
Last modified:November 1, 1997 - v2
Checksum:i06BC0EE689AB3ADE
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti59 – 591A → T in strain: 84-183.
Natural varianti231 – 2311K → R in strain: 84-183.
Natural varianti259 – 2591I → V in strain: 84-183.
Natural varianti376 – 3761N → T in strain: 84-183.
Natural varianti407 – 4071K → Q in strain: 84-183.
Natural varianti443 – 4431L → S in strain: 84-183.
Natural varianti475 – 4751A → V in strain: 84-183.
Natural varianti501 – 5011I → V in strain: 84-183.
Natural varianti607 – 6071R → K in strain: 84-183.
Natural varianti636 – 6372CT → HA in strain: 84-183.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80807 Genomic DNA. Translation: AAC46270.1.
AE000511 Genomic DNA. Translation: AAD08158.1.
PIRiB64659.
RefSeqiNP_207905.1. NC_000915.1.
YP_006935029.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08158; AAD08158; HP_1114.
GeneIDi13870307.
899650.
KEGGiheo:C694_05745.
hpy:HP1114.
PATRICi20593569. VBIHelPyl33062_1164.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80807 Genomic DNA. Translation: AAC46270.1.
AE000511 Genomic DNA. Translation: AAD08158.1.
PIRiB64659.
RefSeqiNP_207905.1. NC_000915.1.
YP_006935029.1. NC_018939.1.

3D structure databases

ProteinModelPortaliP94846.
SMRiP94846. Positions 3-597.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3238N.
IntActiP94846. 18 interactions.
MINTiMINT-171452.
STRINGi85962.HP1114.

Proteomic databases

PRIDEiP94846.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08158; AAD08158; HP_1114.
GeneIDi13870307.
899650.
KEGGiheo:C694_05745.
hpy:HP1114.
PATRICi20593569. VBIHelPyl33062_1164.

Phylogenomic databases

eggNOGiCOG0556.
KOiK03702.
OMAiNKTLCAQ.
OrthoDBiEOG6B360R.

Enzyme and pathway databases

BioCyciHPY:HP1114-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB.
InterProiIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the Helicobacter pylori uvr B gene."
    Thompson S.A., Latch R.L., Blaser J.M.
    Gene 209:113-122(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 53726 / 84-183.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiUVRB_HELPY
AccessioniPrimary (citable) accession number: P94846
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.