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P94846 (UVRB_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UvrABC system protein B

Short name=Protein UvrB
Alternative name(s):
Excinuclease ABC subunit B
Gene names
Name:uvrB
Ordered Locus Names:HP_1114
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length658 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204

Subunit structure

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00204.

Domain

The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204

Sequence similarities

Belongs to the UvrB family.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 UVR domain.

Ontologies

Keywords
   Biological processDNA damage
DNA excision
DNA repair
SOS response
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionExcision nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSOS response

Inferred from electronic annotation. Source: UniProtKB-HAMAP

nucleotide-excision repair

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

excinuclease ABC activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

helicase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 658658UvrABC system protein B HAMAP-Rule MF_00204
PRO_0000138396

Regions

Domain25 – 414390Helicase ATP-binding
Domain433 – 607175Helicase C-terminal
Domain623 – 65836UVR
Nucleotide binding38 – 458ATP Potential
Motif91 – 11424Beta-hairpin HAMAP-Rule MF_00204

Natural variations

Natural variant591A → T in strain: 84-183.
Natural variant2311K → R in strain: 84-183.
Natural variant2591I → V in strain: 84-183.
Natural variant3761N → T in strain: 84-183.
Natural variant4071K → Q in strain: 84-183.
Natural variant4431L → S in strain: 84-183.
Natural variant4751A → V in strain: 84-183.
Natural variant5011I → V in strain: 84-183.
Natural variant6071R → K in strain: 84-183.
Natural variant636 – 6372CT → HA in strain: 84-183.

Sequences

Sequence LengthMass (Da)Tools
P94846 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 06BC0EE689AB3ADE

FASTA65875,916
        10         20         30         40         50         60 
MPLFDLKSPY PPAGDQPQAI EALTKSLKNN NHYQTLVGVT GSGKTYTMAN IIAQTNKPAL 

        70         80         90        100        110        120 
IMSHNKTLCA QLYSEFKAFF PHNRVEYFIS HFDYYQPESY IPRRDLFIEK DSSINDDLER 

       130        140        150        160        170        180 
LRLSATTSLL GYDDVIVIAS VSANYGLGNP EEYLKVMEKI KVGEKRAYKS FLLKLVEMGY 

       190        200        210        220        230        240 
SRNEVVFDRG SFRATGECVD IFPAYNDAEF IRIEFFGDEI ERIAVFDALE KNEIKRLDSV 

       250        260        270        280        290        300 
MLYAASQFAV GSERLNLAIK SIEDELALRL KFFKEQDKML EYNRLKQRTE HDLEMISATG 

       310        320        330        340        350        360 
VCKGIENYAR HFTGKAPNET PFCLFDYLGI FEREFLVIVD ESHVSLPQFG GMYAGDMSRK 

       370        380        390        400        410        420 
SVLVEYGFRL PSALDNRPLK FDEFIHKNCQ FLFVSATPNK LELELSKKNV AEQIIRPTGL 

       430        440        450        460        470        480 
LDPKFEVRDS DKQVQDLFDE IKLVVARGER VLITTLTKKM AEELCKYYAE WGLKARYMHS 

       490        500        510        520        530        540 
EIDAIERNHI IRSLRLKEFD ILIGINLLRE GLDLPEVSLV AIMDADKEGF LRSETSLIQT 

       550        560        570        580        590        600 
MGRAARNANG KVLLYAKKIT QSMQKAFEIT SYRRAKQEEF NKIHNITPKT VTRALEEELK 

       610        620        630        640        650 
LRDDEIRIAK ALKKDKMPKS EREKIIKELD KKMRECTKNL DFEEAMRLRD EIAQLRTL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U80807 Genomic DNA. Translation: AAC46270.1.
AE000511 Genomic DNA. Translation: AAD08158.1.
PIRB64659.
RefSeqNP_207905.1. NC_000915.1.
YP_006935029.1. NC_018939.1.

3D structure databases

ProteinModelPortalP94846.
SMRP94846. Positions 3-597.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-3238N.
IntActP94846. 18 interactions.
MINTMINT-171452.
STRING85962.HP1114.

Proteomic databases

PRIDEP94846.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD08158; AAD08158; HP_1114.
GeneID899650.
KEGGheo:C694_05745.
hpy:HP1114.
PATRIC20593569. VBIHelPyl33062_1164.

Phylogenomic databases

eggNOGCOG0556.
KOK03702.
OMAESGNPLH.
OrthoDBEOG6B360R.

Enzyme and pathway databases

BioCycHPY:HP1114-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPMF_00204. UvrB.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERPTHR24029. PTHR24029. 1 hit.
PfamPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsTIGR00631. uvrb. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUVRB_HELPY
AccessionPrimary (citable) accession number: P94846
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names