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Protein

Glutamine synthetase

Gene

glnA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi139Magnesium 1By similarity1
Metal bindingi141Magnesium 2By similarity1
Metal bindingi223Magnesium 2By similarity1
Metal bindingi230Magnesium 2By similarity1
Binding sitei275L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi279Magnesium 1; via pros nitrogenBy similarity1
Binding sitei283ATPBy similarity1
Binding sitei331L-glutamateBy similarity1
Binding sitei337L-glutamateBy similarity1
Binding sitei349ATPBy similarity1
Binding sitei349L-glutamateBy similarity1
Binding sitei354ATPBy similarity1
Metal bindingi367Magnesium 1By similarity1
Binding sitei369L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi281 – 283ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHPY:HP0512-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseCurated
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:HP_0512
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532391 – 481Glutamine synthetaseAdd BLAST481

Proteomic databases

PaxDbiP94845.

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexameric ring.By similarity

Protein-protein interaction databases

DIPiDIP-3543N.
IntActiP94845. 14 interactors.
MINTiMINT-172713.
STRINGi85962.HP0512.

Structurei

3D structure databases

ProteinModelPortaliP94845.
SMRiP94845.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni274 – 275L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
KOiK01915.
OMAiKVLNQVG.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P94845-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVRTQNSES KIKEFFEFCK ENEVEFVDFR FSDIKGTWNH IAYSFGALTH
60 70 80 90 100
GMLKEGIPFD ASCFKGWQGI EHSDMILTPD LVRYFIDPFS ADVSVVVFCD
110 120 130 140 150
VYDVYKNQPY EKCPRSIAKK ALQHLKDSGL GDVAYFGAEN EFFIFDSIKI
160 170 180 190 200
KDASNSQYYE VDSEEGEWNR DRSFENGVNF GHRPGKQGGY MPVPPTDTMM
210 220 230 240 250
DIRTEIVKVL NQVGLETFVV HHEVAQAQGE VGVKFGDLVE AADNVQKLKY
260 270 280 290 300
VVKMVAHLNG KTATFMPKPL YGDNGSGMHT HVSVWKNNEN LFSGETYKGL
310 320 330 340 350
SEFALHFLGG VLRHARGLAA FTNASTNSYK RLIPGYEAPS ILTYSANNRS
360 370 380 390 400
ASVRIPYGIS KNSARFEFRF PDSSSNPYLA FAAILMAGMD GVKNKIDPGE
410 420 430 440 450
AMDINLFKLT LDEIREKGIK QMPHTLRRSL EEMLADKQYL KESQVFSEEF
460 470 480
IQAYQSLKFN AEVFPWESKP HPFEFITTYS C
Length:481
Mass (Da):54,514
Last modified:November 1, 1997 - v2
Checksum:i87EDFEB540723D3E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti396I → M in AAB39719 (Ref. 2) Curated1
Sequence conflicti443S → G in AAB39719 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07575.1.
U75328 Genomic DNA. Translation: AAB39719.1.
PIRiH64583.
RefSeqiNP_207309.1. NC_000915.1.
WP_000637150.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07575; AAD07575; HP_0512.
GeneIDi900049.
KEGGiheo:C694_02630.
hpy:HP0512.
PATRICi20592299. VBIHelPyl33062_0538.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07575.1.
U75328 Genomic DNA. Translation: AAB39719.1.
PIRiH64583.
RefSeqiNP_207309.1. NC_000915.1.
WP_000637150.1. NC_018939.1.

3D structure databases

ProteinModelPortaliP94845.
SMRiP94845.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3543N.
IntActiP94845. 14 interactors.
MINTiMINT-172713.
STRINGi85962.HP0512.

Proteomic databases

PaxDbiP94845.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07575; AAD07575; HP_0512.
GeneIDi900049.
KEGGiheo:C694_02630.
hpy:HP0512.
PATRICi20592299. VBIHelPyl33062_0538.

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
KOiK01915.
OMAiKVLNQVG.

Enzyme and pathway databases

BioCyciHPY:HP0512-MONOMER.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLN1B_HELPY
AccessioniPrimary (citable) accession number: P94845
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 12, 2017
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.