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P94844 (DAPB_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxy-tetrahydrodipicolinate reductase
Short name=HTPA reductase
EC=1.17.1.8
Gene names
Name:dapB
Ordered Locus Names:HP_0510
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter ›

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate By similarity. HAMAP-Rule MF_00102

Catalytic activity

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H. HAMAP-Rule MF_00102

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4. HAMAP-Rule MF_00102

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the DapB family.

Caution

Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli (PubMed:20503968) that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2542544-hydroxy-tetrahydrodipicolinate reductase HAMAP-Rule MF_00102
PRO_0000141445

Regions

Nucleotide binding7 – 126NAD(P) By similarity
Nucleotide binding91 – 933NAD(P) By similarity
Nucleotide binding115 – 1184NAD(P) By similarity
Region157 – 1582Substrate binding By similarity

Sites

Active site1471Proton donor/acceptor By similarity
Active site1511Proton donor By similarity
Binding site351NADP By similarity
Binding site1481Substrate By similarity

Experimental info

Sequence conflict221G → E in AAB39718. Ref.1
Sequence conflict281A → V in AAB39718. Ref.1
Sequence conflict441S → A in AAB39718. Ref.1
Sequence conflict761N → H in AAB39718. Ref.1
Sequence conflict801A → T in AAB39718. Ref.1
Sequence conflict1041Q → K in AAB39718. Ref.1
Sequence conflict1151A → V in AAB39718. Ref.1
Sequence conflict1201I → L in AAB39718. Ref.1
Sequence conflict1321T → A in AAB39718. Ref.1
Sequence conflict1441I → V in AAB39718. Ref.1
Sequence conflict1541I → A in AAB39718. Ref.1
Sequence conflict1791I → T in AAB39718. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P94844 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 96DBC96B76C5BE3D

FASTA25427,827
        10         20         30         40         50         60 
MKIGVYGASG RIGKLLLEEL KGGYKGLALS SVFVRQKCET DFSSFSHAPL VTNDLKAFVR 

        70         80         90        100        110        120 
ACECVIDFSL PKGVDNLLEA LLECPKILVS GTTGLEKETL EKMQQLALKA PLLHAHNMSI 

       130        140        150        160        170        180 
GIMMLNQLAF LTSLKLKDAD IEIIETHHNL KKDIPSGTAL SLYETCAKAR GYDEKNALIT 

       190        200        210        220        230        240 
HREGLRSKES IGIAALRGGD VAGKHTIGFY LEGEYIELSH TATNRSIFAK GALEVALWLK 

       250 
DKAAKKYEIN EMFG

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the dihydrodipicolinate reductase gene (dapB) from Helicobacter pylori and complementation in Escherichia coli."
Clairoux N., Boissinot M.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 4867 / CCUG 17874 / NCTC 11638.
[2]"The complete genome sequence of the gastric pathogen Helicobacter pylori."
Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. expand/collapse author list , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.
Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700392 / 26695.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U75328 Genomic DNA. Translation: AAB39718.1.
AE000511 Genomic DNA. Translation: AAD07574.1.
PIRF64583.
RefSeqNP_207307.1. NC_000915.1.
YP_006934430.1. NC_018939.1.

3D structure databases

ProteinModelPortalP94844.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-3440N.
MINTMINT-169897.
STRING85962.HP0510.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD07574; AAD07574; HP_0510.
GeneID13869698.
900344.
KEGGheo:C694_02620.
hpy:HP0510.
PATRIC20592295. VBIHelPyl33062_0536.

Phylogenomic databases

eggNOGCOG0289.
KOK00215.
OMAFYLEGEY.
ProtClustDBPRK00048.

Enzyme and pathway databases

UniPathwayUPA00034; UER00018.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00102. DapB.
InterProIPR022663. DapB_C.
IPR000846. DapB_N.
IPR022664. DapB_N_CS.
IPR011770. Dihydrodipicolinate_Rdtase.
IPR023940. Dihydrodipicolinate_Rdtase_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR20836. PTHR20836. 1 hit.
PfamPF05173. DapB_C. 1 hit.
PF01113. DapB_N. 1 hit.
[Graphical view]
PIRSFPIRSF000161. DHPR. 1 hit.
TIGRFAMsTIGR00036. dapB. 1 hit.
PROSITEPS01298. DAPB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPB_HELPY
AccessionPrimary (citable) accession number: P94844
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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