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P94692

- P94692_DESAF

UniProt

P94692 - P94692_DESAF

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Protein
Pyruvate-flavodoxin oxidoreductase
Gene
por
Organism
Desulfovibrio africanus
Status
Unreviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Oxidoreductase required for the transfer of electrons from pyruvate to flavodoxin By similarity.UniRule annotation

Catalytic activityi

Pyruvate + CoA + oxidized flavodoxin = acetyl-CoA + CO2 + reduced flavodoxin.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311Pyruvate 1Imported
Binding sitei31 – 311Pyruvate 2Imported
Sitei31 – 311Important for catalytic activity
Binding sitei64 – 641Thiamine pyrophosphateImported
Sitei64 – 641Important for catalytic activity
Binding sitei88 – 881Thiamine pyrophosphateImported
Binding sitei114 – 1141Pyruvate 1Imported
Binding sitei114 – 1141Pyruvate 2Imported
Sitei114 – 1141Important for catalytic activity
Metal bindingi689 – 6891Iron-sulfur (4Fe-4S) 3Imported
Metal bindingi690 – 6901Iron-sulfur (4Fe-4S) 3; via amide nitrogenImported
Metal bindingi692 – 6921Iron-sulfur (4Fe-4S) 3Imported
Metal bindingi693 – 6931Iron-sulfur (4Fe-4S) 3; via amide nitrogenImported
Metal bindingi695 – 6951Iron-sulfur (4Fe-4S) 3Imported
Metal bindingi699 – 6991Iron-sulfur (4Fe-4S) 2Imported
Metal bindingi745 – 7451Iron-sulfur (4Fe-4S) 2Imported
Metal bindingi748 – 7481Iron-sulfur (4Fe-4S) 2Imported
Metal bindingi749 – 7491Iron-sulfur (4Fe-4S) 2; via amide nitrogenImported
Metal bindingi751 – 7511Iron-sulfur (4Fe-4S) 2Imported
Metal bindingi755 – 7551Iron-sulfur (4Fe-4S) 3Imported
Metal bindingi761 – 7611Iron-sulfur (4Fe-4S) 3Imported
Metal bindingi812 – 8121Iron-sulfur (4Fe-4S) 1Imported
Metal bindingi815 – 8151Iron-sulfur (4Fe-4S) 1Imported
Binding sitei817 – 8171Thiamine pyrophosphateImported
Metal bindingi840 – 8401Iron-sulfur (4Fe-4S) 1Imported
Binding sitei840 – 8401Thiamine pyrophosphateImported
Binding sitei869 – 8691Thiamine pyrophosphateImported
Metal bindingi963 – 9631MagnesiumImported
Metal bindingi983 – 9831CalciumImported
Metal bindingi985 – 9851CalciumImported
Metal bindingi991 – 9911MagnesiumImported
Metal bindingi993 – 9931MagnesiumImported
Binding sitei996 – 9961Pyruvate 1Imported
Sitei996 – 9961Important for catalytic activity
Metal bindingi1056 – 10561Calcium; via carbonyl oxygenImported
Metal bindingi1057 – 10571Calcium; via carbonyl oxygenImported
Metal bindingi1059 – 10591Calcium; via carbonyl oxygenImported
Metal bindingi1061 – 10611Calcium; via carbonyl oxygenImported
Metal bindingi1063 – 10631CalciumImported
Metal bindingi1071 – 10711Iron-sulfur (4Fe-4S) 1Imported
Binding sitei1202 – 12021Pyruvate 1Imported

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. iron ion binding Source: InterPro
  3. oxidoreductase activity, acting on the aldehyde or oxo group of donors Source: InterPro
  4. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. electron transport chain Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Electron transportUniRule annotation, Transport

Keywords - Ligandi

4Fe-4SUniRule annotationImported, CalciumImported, Iron, Iron-sulfur, MagnesiumImported, Metal-binding, PyruvateImported, Thiamine pyrophosphateImported

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate-flavodoxin oxidoreductaseUniRule annotation (EC:1.2.7.-UniRule annotation)
Gene namesi
Name:porImported
OrganismiDesulfovibrio africanusImported
Taxonomic identifieri873 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio

Interactioni

Protein-protein interaction databases

DIPiDIP-29036N.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B0PX-ray2.31A/B2-1232[»]
1KEKX-ray1.90A/B2-1232[»]
2C3MX-ray1.84A/B2-1232[»]
2C3OX-ray2.70A/B2-1232[»]
2C3PX-ray2.33A/B2-1232[»]
2C3UX-ray2.32A/B2-1232[»]
2C3YX-ray1.93A/B2-1232[»]
2C42X-ray1.78A/B2-1232[»]
2PDAX-ray3.00A/B2-1232[»]
2UZAX-ray2.42A/B2-1232[»]
ProteinModelPortaliP94692.
SMRiP94692. Positions 2-1232.

Miscellaneous databases

EvolutionaryTraceiP94692.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni962 – 9654Thiamine pyrophosphate bindingImported
Regioni991 – 9966Thiamine pyrophosphate bindingImported

Sequence similaritiesi

Belongs to the nifJ family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 2 hits.
3.40.920.10. 1 hit.
4.10.780.10. 1 hit.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR019456. Pyrv-flavodox_OxRtase_EKR.
IPR019752. Pyrv/ketoisovalerate_OxRed_cat.
IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N.
IPR011895. Pyrv_flavodox_OxRed.
IPR002869. Pyrv_flavodox_OxRed_cen.
IPR029061. THDP-binding.
IPR011766. TPP_enzyme-bd_C.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
[Graphical view]
PfamiPF10371. EKR. 1 hit.
PF00037. Fer4. 1 hit.
PF01558. POR. 1 hit.
PF01855. POR_N. 1 hit.
PF02775. TPP_enzyme_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000159. NifJ. 1 hit.
SMARTiSM00890. EKR. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 2 hits.
SSF52922. SSF52922. 1 hit.
SSF53323. SSF53323. 1 hit.
TIGRFAMsiTIGR02176. pyruv_ox_red. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P94692-1 [UniParc]FASTAAdd to Basket

« Hide

MGKKMMTTDG NTATAHVAYA MSEVAAIYPI TPSSTMGEEA DDWAAQGRKN     50
IFGQTLTIRE MQSEAGAAGA VHGALAAGAL TTTFTASQGL LLMIPNMYKI 100
SGELLPGVFH VTARAIAAHA LSIFGDHQDI YAARQTGFAM LASSSVQEAH 150
DMALVAHLAA IESNVPFMHF FDGFRTSHEI QKIEVLDYAD MASLVNQKAL 200
AEFRAKSMNP EHPHVRGTAQ NPDIYFQGRE AANPYYLKVP GIVAEYMQKV 250
ASLTGRSYKL FDYVGAPDAE RVIVSMGSSC ETIEEVINHL AAKGEKIGLI 300
KVRLYRPFVS EAFFAALPAS AKVITVLDRT KEPGAPGDPL YLDVCSAFVE 350
RGEAMPKILA GRYGLGSKEF SPAMVKSVYD NMSGAKKNHF TVGIEDDVTG 400
TSLPVDNAFA DTTPKGTIQC QFWGLGADGT VGANKQAIKI IGDNTDLFAQ 450
GYFSYDSKKS GGITISHLRF GEKPIQSTYL VNRADYVACH NPAYVGIYDI 500
LEGIKDGGTF VLNSPWSSLE DMDKHLPSGI KRTIANKKLK FYNIDAVKIA 550
TDVGLGGRIN MIMQTAFFKL AGVLPFEKAV DLLKKSIHKA YGKKGEKIVK 600
MNTDAVDQAV TSLQEFKYPD SWKDAPAETK AEPMTNEFFK NVVKPILTQQ 650
GDKLPVSAFE ADGRFPLGTS QFEKRGVAIN VPQWVPENCI QCNQCAFVCP 700
HSAILPVLAK EEELVGAPAN FTALEAKGKE LKGYKFRIQI NTLDCMGCGN 750
CADICPPKEK ALVMQPLDTQ RDAQVPNLEY AARIPVKSEV LPRDSLKGSQ 800
FQEPLMEFSG ACSGCGETPY VRVITQLFGE RMFIANATGC SSIWGASAPS 850
MPYKTNRLGQ GPAWGNSLFE DAAEYGFGMN MSMFARRTHL ADLAAKALES 900
DASGDVKEAL QGWLAGKNDP IKSKEYGDKL KKLLAGQKDG LLGQIAAMSD 950
LYTKKSVWIF GGDGWAYDIG YGGLDHVLAS GEDVNVFVMD TEVYSNTGGQ 1000
SSKATPTGAV AKFAAAGKRT GKKDLARMVM TYGYVYVATV SMGYSKQQFL 1050
KVLKEAESFP GPSLVIAYAT CINQGLRKGM GKSQDVMNTA VKSGYWPLFR 1100
YDPRLAAQGK NPFQLDSKAP DGSVEEFLMA QNRFAVLDRS FPEDAKRLRA 1150
QVAHELDVRF KELEHMAATN IFESFAPAGG KADGSVDFGE GAEFCTRDDT 1200
PMMARPDSGE ACDQNRAGTS EQQGDLSKRT KK 1232
Length:1,232
Mass (Da):133,680
Last modified:May 1, 1997 - v1
Checksum:i55D84F536CCFFDBF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09702 Genomic DNA. Translation: CAA70873.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09702 Genomic DNA. Translation: CAA70873.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1B0P X-ray 2.31 A/B 2-1232 [» ]
1KEK X-ray 1.90 A/B 2-1232 [» ]
2C3M X-ray 1.84 A/B 2-1232 [» ]
2C3O X-ray 2.70 A/B 2-1232 [» ]
2C3P X-ray 2.33 A/B 2-1232 [» ]
2C3U X-ray 2.32 A/B 2-1232 [» ]
2C3Y X-ray 1.93 A/B 2-1232 [» ]
2C42 X-ray 1.78 A/B 2-1232 [» ]
2PDA X-ray 3.00 A/B 2-1232 [» ]
2UZA X-ray 2.42 A/B 2-1232 [» ]
ProteinModelPortali P94692.
SMRi P94692. Positions 2-1232.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29036N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P94692.

Family and domain databases

Gene3Di 3.40.50.920. 1 hit.
3.40.50.970. 2 hits.
3.40.920.10. 1 hit.
4.10.780.10. 1 hit.
InterProi IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR019456. Pyrv-flavodox_OxRtase_EKR.
IPR019752. Pyrv/ketoisovalerate_OxRed_cat.
IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N.
IPR011895. Pyrv_flavodox_OxRed.
IPR002869. Pyrv_flavodox_OxRed_cen.
IPR029061. THDP-binding.
IPR011766. TPP_enzyme-bd_C.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
[Graphical view ]
Pfami PF10371. EKR. 1 hit.
PF00037. Fer4. 1 hit.
PF01558. POR. 1 hit.
PF01855. POR_N. 1 hit.
PF02775. TPP_enzyme_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF000159. NifJ. 1 hit.
SMARTi SM00890. EKR. 1 hit.
[Graphical view ]
SUPFAMi SSF52518. SSF52518. 2 hits.
SSF52922. SSF52922. 1 hit.
SSF53323. SSF53323. 1 hit.
TIGRFAMsi TIGR02176. pyruv_ox_red. 1 hit.
PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Claude H.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: NCIB 8401Imported.
  2. "Isolation and analysis of the gene encoding the pyruvate-ferredoxin oxidoreductase of Desulfovibrio africanus, production of the recombinant enzyme in Escherichia coli, and effect of carboxy-terminal deletions on its stability."
    Pieulle L., Magro V., Hatchikian E.C.
    J. Bacteriol. 179:5684-5692(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: NCIB 8401Imported.
  3. "Crystal structures of the key anaerobic enzyme pyruvate:ferredoxin oxidoreductase, free and in complex with pyruvate."
    Chabriere E., Charon M.H., Volbeda A., Pieulle L., Hatchikian E.C., Fontecilla-Camps J.C.
    Nat. Struct. Biol. 6:182-190(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS) OF 2-1232 IN COMPLEX WITH CALCIUM; IRON-SULFUR (4FE-4S); MAGNESIUM; PYRUVATE AND THIAMINE PYROPHOSPHATE, ACTIVE SITE.
  4. "Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase."
    Chabriere E., Vernede X., Guigliarelli B., Charon M.H., Hatchikian E.C., Fontecilla-Camps J.C.
    Science 294:2559-2563(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 2-1232 IN COMPLEX WITH CALCIUM; IRON-SULFUR (4FE-4S) AND MAGNESIUM.
  5. "Flexibility of thiamine diphosphate revealed by kinetic crystallographic studies of the reaction of pyruvate-ferredoxin oxidoreductase with pyruvate."
    Cavazza C., Contreras-Martel C., Pieulle L., Chabriere E., Hatchikian E.C., Fontecilla-Camps J.C.
    Structure 14:217-224(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 2-1232 IN COMPLEX WITH CALCIUM; IRON-SULFUR (4FE-4S); MAGNESIUM; PYRUVATE AND THIAMINE PYROPHOSPHATE.
  6. "Pyruvate:Ferredoxin Oxidoreductase."
    Chabriere E., Cavazza C., Contreras-Martel C., Fontecilla-Camps J.C.
    Submitted (APR-2007) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.42 ANGSTROMS) OF 2-1232 IN COMPLEX WITH CALCIUM; IRON-SULFUR (4FE-4S) AND MAGNESIUM.

Entry informationi

Entry nameiP94692_DESAF
AccessioniPrimary (citable) accession number: P94692
Entry historyi
Integrated into UniProtKB/TrEMBL: May 1, 1997
Last sequence update: May 1, 1997
Last modified: September 3, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3

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