Unreviewed, UniProtKB/TrEMBL P94692 (P94692_DESAF)

Names and origin

Protein names

Submitted name:
    Pyruvate-ferredoxin oxidoreductase EMBL CAA70873.1

Gene names

Name:

por EMBL CAA70873.1

Organism

Desulfovibrio africanus EMBL CAA70873.1

Taxonomic identifier

873 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	1232 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

Ontologies

Keywords
Ligand	Pyruvate EMBL CAA70873.1
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on the aldehyde or oxo group of donors Inferred from electronic annotation. Source: InterPro thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	P94692-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 55D84F536CCFFDBF	FASTA	1,232	133,680
	10 20 30 40 50 60 MGKKMMTTDG NTATAHVAYA MSEVAAIYPI TPSSTMGEEA DDWAAQGRKN IFGQTLTIRE 70 80 90 100 110 120 MQSEAGAAGA VHGALAAGAL TTTFTASQGL LLMIPNMYKI SGELLPGVFH VTARAIAAHA 130 140 150 160 170 180 LSIFGDHQDI YAARQTGFAM LASSSVQEAH DMALVAHLAA IESNVPFMHF FDGFRTSHEI 190 200 210 220 230 240 QKIEVLDYAD MASLVNQKAL AEFRAKSMNP EHPHVRGTAQ NPDIYFQGRE AANPYYLKVP 250 260 270 280 290 300 GIVAEYMQKV ASLTGRSYKL FDYVGAPDAE RVIVSMGSSC ETIEEVINHL AAKGEKIGLI 310 320 330 340 350 360 KVRLYRPFVS EAFFAALPAS AKVITVLDRT KEPGAPGDPL YLDVCSAFVE RGEAMPKILA 370 380 390 400 410 420 GRYGLGSKEF SPAMVKSVYD NMSGAKKNHF TVGIEDDVTG TSLPVDNAFA DTTPKGTIQC 430 440 450 460 470 480 QFWGLGADGT VGANKQAIKI IGDNTDLFAQ GYFSYDSKKS GGITISHLRF GEKPIQSTYL 490 500 510 520 530 540 VNRADYVACH NPAYVGIYDI LEGIKDGGTF VLNSPWSSLE DMDKHLPSGI KRTIANKKLK 550 560 570 580 590 600 FYNIDAVKIA TDVGLGGRIN MIMQTAFFKL AGVLPFEKAV DLLKKSIHKA YGKKGEKIVK 610 620 630 640 650 660 MNTDAVDQAV TSLQEFKYPD SWKDAPAETK AEPMTNEFFK NVVKPILTQQ GDKLPVSAFE 670 680 690 700 710 720 ADGRFPLGTS QFEKRGVAIN VPQWVPENCI QCNQCAFVCP HSAILPVLAK EEELVGAPAN 730 740 750 760 770 780 FTALEAKGKE LKGYKFRIQI NTLDCMGCGN CADICPPKEK ALVMQPLDTQ RDAQVPNLEY 790 800 810 820 830 840 AARIPVKSEV LPRDSLKGSQ FQEPLMEFSG ACSGCGETPY VRVITQLFGE RMFIANATGC 850 860 870 880 890 900 SSIWGASAPS MPYKTNRLGQ GPAWGNSLFE DAAEYGFGMN MSMFARRTHL ADLAAKALES 910 920 930 940 950 960 DASGDVKEAL QGWLAGKNDP IKSKEYGDKL KKLLAGQKDG LLGQIAAMSD LYTKKSVWIF 970 980 990 1000 1010 1020 GGDGWAYDIG YGGLDHVLAS GEDVNVFVMD TEVYSNTGGQ SSKATPTGAV AKFAAAGKRT 1030 1040 1050 1060 1070 1080 GKKDLARMVM TYGYVYVATV SMGYSKQQFL KVLKEAESFP GPSLVIAYAT CINQGLRKGM 1090 1100 1110 1120 1130 1140 GKSQDVMNTA VKSGYWPLFR YDPRLAAQGK NPFQLDSKAP DGSVEEFLMA QNRFAVLDRS 1150 1160 1170 1180 1190 1200 FPEDAKRLRA QVAHELDVRF KELEHMAATN IFESFAPAGG KADGSVDFGE GAEFCTRDDT 1210 1220 1230 PMMARPDSGE ACDQNRAGTS EQQGDLSKRT KK

References

[1]	"Isolation and analysis of the gene encoding the pyruvate-ferredoxin oxidoreductase of Desulfovibrio africanus, production of the recombinant enzyme in Escherichia coli, and effect of carboxy-terminal deletions on its stability." Pieulle L., Magro V., Hatchikian E.C. J. Bacteriol. 179:5684-5692(1997) [PubMed: 9294422] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: NCIB 8401 EMBL CAA70873.1.
[2]	Claude H. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: NCIB 8401 EMBL CAA70873.1.
[3]	"Crystal structures of the key anaerobic enzyme pyruvate:ferredoxin oxidoreductase, free and in complex with pyruvate." Chabriere E., Charon M.H., Volbeda A., Pieulle L., Hatchikian E.C., Fontecilla-Camps J.C. Nat. Struct. Biol. 6:182-190(1999) [PubMed: 10048931] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS) OF 2-1232.
[4]	"Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase." Chabriere E., Vernede X., Guigliarelli B., Charon M.H., Hatchikian E.C., Fontecilla-Camps J.C. Science 294:2559-2563(2001) [PubMed: 11752578] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 2-1232.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y09702 Genomic DNA. Translation: CAA70873.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B0P	X-ray	2.31	A/B	2-1232	[»]
1KEK	X-ray	1.90	A/B	2-1232	[»]
2C3M	X-ray	1.84	A/B	2-1232	[»]
2C3O	X-ray	2.70	A/B	2-1232	[»]
2C3P	X-ray	2.33	A/B	2-1232	[»]
2C3U	X-ray	2.32	A/B	2-1232	[»]
2C3Y	X-ray	1.93	A/B	2-1232	[»]
2C42	X-ray	1.78	A/B	2-1232	[»]
2PDA	X-ray	3.00	A/B	2-1232	[»]
2UZA	X-ray	2.42	A/B	2-1232	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29036N.

Family and domain databases

InterPro

IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR019456. Pyrv-flavodox_OxRtase_EKR.
IPR019752. Pyrv/ketoisovalerate_OxRed_cat.
IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N.
IPR011895. Pyrv_flavodox_OxRed.
IPR002869. Pyrv_flavodox_OxRed_cen.
IPR011766. TPP_enzyme_bd_C.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR015941. Transketolase-like_C.
[Graphical view]

Gene3D

G3DSA:3.40.920.10. Pyrv_Fd/Flavodoxin_OxRdtase. 1 hit.
G3DSA:3.40.50.920. Transketo_C_like. 1 hit.

Pfam

PF10371. EKR. 1 hit.
PF01558. POR. 1 hit.
PF01855. POR_N. 1 hit.
PF02775. TPP_enzyme_C. 1 hit.
[Graphical view]

PIRSF

PIRSF000159. NifJ. 1 hit.

SMART

SM00890. EKR. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02176. pyruv_ox_red. 1 hit.

PROSITE

PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

P94692_DESAF

Accession

Primary (citable) accession number: P94692

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 1997
Last sequence update:	May 1, 1997
Last modified:	February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)