Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P94692 (P94692_DESAF) Unreviewed, UniProtKB/TrEMBL

Last modified December 11, 2013. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate-flavodoxin oxidoreductase PIRNR PIRNR000159

EC=1.2.7.- PIRNR PIRNR000159
Gene names
Name:por EMBL CAA70873.1
OrganismDesulfovibrio africanus EMBL CAA70873.1
Taxonomic identifier873 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio

Protein attributes

Sequence length1232 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Oxidoreductase required for the transfer of electrons from pyruvate to flavodoxin By similarity. PIRNR PIRNR000159

Catalytic activity

Pyruvate + CoA + oxidized flavodoxin = acetyl-CoA + CO2 + reduced flavodoxin. PIRNR PIRNR000159

Sequence similarities

Belongs to the nifJ family. PIRNR PIRNR000159

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region962 – 9654Thiamine pyrophosphate binding PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 1B0P
Region991 – 9966Thiamine pyrophosphate binding PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 1B0P

Sites

Metal binding6891Iron-sulfur (4Fe-4S) 3 PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding6901Iron-sulfur (4Fe-4S) 3; via amide nitrogen PDB 2PDA
Metal binding6921Iron-sulfur (4Fe-4S) 3 PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding6931Iron-sulfur (4Fe-4S) 3; via amide nitrogen PDB 2C3M PDB 2C3O PDB 2C42 PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 2UZA
Metal binding6951Iron-sulfur (4Fe-4S) 3 PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding6991Iron-sulfur (4Fe-4S) 2 PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding7451Iron-sulfur (4Fe-4S) 2 PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding7481Iron-sulfur (4Fe-4S) 2 PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding7491Iron-sulfur (4Fe-4S) 2; via amide nitrogen PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding7511Iron-sulfur (4Fe-4S) 2 PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding7551Iron-sulfur (4Fe-4S) 3 PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding7611Iron-sulfur (4Fe-4S) 3 PDB 2PDA
Metal binding8121Iron-sulfur (4Fe-4S) 1 PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding8151Iron-sulfur (4Fe-4S) 1 PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding8401Iron-sulfur (4Fe-4S) 1 PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding9631Magnesium PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding9831Calcium PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding9851Calcium PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding9911Magnesium PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding9931Magnesium PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding10561Calcium; via carbonyl oxygen PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding10571Calcium; via carbonyl oxygen PDB 2C3O PDB 1B0P
Metal binding10591Calcium; via carbonyl oxygen PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding10611Calcium; via carbonyl oxygen PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Metal binding10631Calcium PDB 2C3M PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3Y
Metal binding10711Iron-sulfur (4Fe-4S) 1 PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 2C3P PDB 2C3U PDB 2C3Y PDB 1B0P PDB 1KEK PDB 2UZA
Binding site311Chloride PDB 2C3M
Binding site311Pyruvate 1 PDB 2C3O PDB 2C42 PDB 2C3U
Binding site311Pyruvate 2 PDB 2PDA
Binding site641Thiamine pyrophosphate PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 1B0P
Binding site881Thiamine pyrophosphate PDB 2C3M PDB 2C3O PDB 2C42 PDB 1B0P
Binding site1141Pyruvate 1 PDB 2C3O PDB 2C42 PDB 2C3U
Binding site1141Pyruvate 2 PDB 2PDA
Binding site8171Thiamine pyrophosphate PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 1B0P
Binding site8401Thiamine pyrophosphate PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 1B0P
Binding site8691Thiamine pyrophosphate PDB 2C3M PDB 2C3O PDB 2C42 PDB 2PDA PDB 1B0P
Binding site9961Pyruvate 1 PDB 2C42
Binding site12021Pyruvate 1 PDB 2C3O

Sequences

Sequence LengthMass (Da)Tools
P94692 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 55D84F536CCFFDBF

FASTA1,232133,680
        10         20         30         40         50         60 
MGKKMMTTDG NTATAHVAYA MSEVAAIYPI TPSSTMGEEA DDWAAQGRKN IFGQTLTIRE 

        70         80         90        100        110        120 
MQSEAGAAGA VHGALAAGAL TTTFTASQGL LLMIPNMYKI SGELLPGVFH VTARAIAAHA 

       130        140        150        160        170        180 
LSIFGDHQDI YAARQTGFAM LASSSVQEAH DMALVAHLAA IESNVPFMHF FDGFRTSHEI 

       190        200        210        220        230        240 
QKIEVLDYAD MASLVNQKAL AEFRAKSMNP EHPHVRGTAQ NPDIYFQGRE AANPYYLKVP 

       250        260        270        280        290        300 
GIVAEYMQKV ASLTGRSYKL FDYVGAPDAE RVIVSMGSSC ETIEEVINHL AAKGEKIGLI 

       310        320        330        340        350        360 
KVRLYRPFVS EAFFAALPAS AKVITVLDRT KEPGAPGDPL YLDVCSAFVE RGEAMPKILA 

       370        380        390        400        410        420 
GRYGLGSKEF SPAMVKSVYD NMSGAKKNHF TVGIEDDVTG TSLPVDNAFA DTTPKGTIQC 

       430        440        450        460        470        480 
QFWGLGADGT VGANKQAIKI IGDNTDLFAQ GYFSYDSKKS GGITISHLRF GEKPIQSTYL 

       490        500        510        520        530        540 
VNRADYVACH NPAYVGIYDI LEGIKDGGTF VLNSPWSSLE DMDKHLPSGI KRTIANKKLK 

       550        560        570        580        590        600 
FYNIDAVKIA TDVGLGGRIN MIMQTAFFKL AGVLPFEKAV DLLKKSIHKA YGKKGEKIVK 

       610        620        630        640        650        660 
MNTDAVDQAV TSLQEFKYPD SWKDAPAETK AEPMTNEFFK NVVKPILTQQ GDKLPVSAFE 

       670        680        690        700        710        720 
ADGRFPLGTS QFEKRGVAIN VPQWVPENCI QCNQCAFVCP HSAILPVLAK EEELVGAPAN 

       730        740        750        760        770        780 
FTALEAKGKE LKGYKFRIQI NTLDCMGCGN CADICPPKEK ALVMQPLDTQ RDAQVPNLEY 

       790        800        810        820        830        840 
AARIPVKSEV LPRDSLKGSQ FQEPLMEFSG ACSGCGETPY VRVITQLFGE RMFIANATGC 

       850        860        870        880        890        900 
SSIWGASAPS MPYKTNRLGQ GPAWGNSLFE DAAEYGFGMN MSMFARRTHL ADLAAKALES 

       910        920        930        940        950        960 
DASGDVKEAL QGWLAGKNDP IKSKEYGDKL KKLLAGQKDG LLGQIAAMSD LYTKKSVWIF 

       970        980        990       1000       1010       1020 
GGDGWAYDIG YGGLDHVLAS GEDVNVFVMD TEVYSNTGGQ SSKATPTGAV AKFAAAGKRT 

      1030       1040       1050       1060       1070       1080 
GKKDLARMVM TYGYVYVATV SMGYSKQQFL KVLKEAESFP GPSLVIAYAT CINQGLRKGM 

      1090       1100       1110       1120       1130       1140 
GKSQDVMNTA VKSGYWPLFR YDPRLAAQGK NPFQLDSKAP DGSVEEFLMA QNRFAVLDRS 

      1150       1160       1170       1180       1190       1200 
FPEDAKRLRA QVAHELDVRF KELEHMAATN IFESFAPAGG KADGSVDFGE GAEFCTRDDT 

      1210       1220       1230 
PMMARPDSGE ACDQNRAGTS EQQGDLSKRT KK 

« Hide

References

[1]Claude H.
Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: NCIB 8401 EMBL CAA70873.1.
[2]"Isolation and analysis of the gene encoding the pyruvate-ferredoxin oxidoreductase of Desulfovibrio africanus, production of the recombinant enzyme in Escherichia coli, and effect of carboxy-terminal deletions on its stability."
Pieulle L., Magro V., Hatchikian E.C.
J. Bacteriol. 179:5684-5692(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: NCIB 8401 EMBL CAA70873.1.
[3]"Crystal structures of the key anaerobic enzyme pyruvate:ferredoxin oxidoreductase, free and in complex with pyruvate."
Chabriere E., Charon M.H., Volbeda A., Pieulle L., Hatchikian E.C., Fontecilla-Camps J.C.
Nat. Struct. Biol. 6:182-190(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS) OF 2-1232 IN COMPLEX WITH CALCIUM; IRON-SULFUR (4FE-4S); MAGNESIUM; PYRUVATE AND THIAMINE PYROPHOSPHATE.
[4]"Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase."
Chabriere E., Vernede X., Guigliarelli B., Charon M.H., Hatchikian E.C., Fontecilla-Camps J.C.
Science 294:2559-2563(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 2-1232 IN COMPLEX WITH CALCIUM; IRON-SULFUR (4FE-4S) AND MAGNESIUM.
[5]"Flexibility of thiamine diphosphate revealed by kinetic crystallographic studies of the reaction of pyruvate-ferredoxin oxidoreductase with pyruvate."
Cavazza C., Contreras-Martel C., Pieulle L., Chabriere E., Hatchikian E.C., Fontecilla-Camps J.C.
Structure 14:217-224(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 2-1232 IN COMPLEX WITH CALCIUM; CHLORIDE; IRON-SULFUR (4FE-4S); MAGNESIUM; PYRUVATE AND THIAMINE PYROPHOSPHATE.
[6]"Pyruvate:Ferredoxin Oxidoreductase."
Chabriere E., Cavazza C., Contreras-Martel C., Fontecilla-Camps J.C.
Submitted (APR-2007) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.42 ANGSTROMS) OF 2-1232 IN COMPLEX WITH CALCIUM; IRON-SULFUR (4FE-4S) AND MAGNESIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y09702 Genomic DNA. Translation: CAA70873.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B0PX-ray2.31A/B2-1232[»]
1KEKX-ray1.90A/B2-1232[»]
2C3MX-ray1.84A/B2-1232[»]
2C3OX-ray2.70A/B2-1232[»]
2C3PX-ray2.33A/B2-1232[»]
2C3UX-ray2.32A/B2-1232[»]
2C3YX-ray1.93A/B2-1232[»]
2C42X-ray1.78A/B2-1232[»]
2PDAX-ray3.00A/B2-1232[»]
2UZAX-ray2.42A/B2-1232[»]
ProteinModelPortalP94692.
SMRP94692. Positions 2-1232.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-29036N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.920. 1 hit.
3.40.920.10. 1 hit.
4.10.780.10. 1 hit.
InterProIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR019456. Pyrv-flavodox_OxRtase_EKR.
IPR019752. Pyrv/ketoisovalerate_OxRed_cat.
IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N.
IPR011895. Pyrv_flavodox_OxRed.
IPR002869. Pyrv_flavodox_OxRed_cen.
IPR011766. TPP_enzyme-bd_C.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
[Graphical view]
PfamPF10371. EKR. 1 hit.
PF00037. Fer4. 1 hit.
PF01558. POR. 1 hit.
PF01855. POR_N. 1 hit.
PF02775. TPP_enzyme_C. 1 hit.
[Graphical view]
PIRSFPIRSF000159. NifJ. 1 hit.
SMARTSM00890. EKR. 1 hit.
[Graphical view]
SUPFAMSSF52922. SSF52922. 1 hit.
SSF53323. SSF53323. 1 hit.
TIGRFAMsTIGR02176. pyruv_ox_red. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP94692.

Entry information

Entry nameP94692_DESAF
AccessionPrimary (citable) accession number: P94692
Entry history
Integrated into UniProtKB/TrEMBL: May 1, 1997
Last sequence update: May 1, 1997
Last modified: December 11, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)