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P94692

- POR_DESAF

UniProt

P94692 - POR_DESAF

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Protein

Pyruvate synthase

Gene

por

Organism
Desulfovibrio africanus
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the ferredoxin-dependent oxidative decarboxylation of pyruvate. Required for the transfer of electrons from pyruvate to ferredoxin (PubMed:9294422, PubMed:7612653). Ferredoxin I and ferredoxin II, which are single 4Fe-4S cluster ferredoxins are the most effective electron carriers of POR (PubMed:7612653).1 Publication

Catalytic activityi

Pyruvate + CoA + 2 oxidized ferredoxin = acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+.1 Publication

Cofactori

Binds 3 4Fe-4S clusters per subunit.4 Publications
Binds 1 thiamine pyrophosphate per subunit.3 Publications
Binds 1 calcium ion per subunit.3 Publications
Binds 1 magnesium ion per subunit.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311Pyruvate2 Publications
Sitei31 – 311Important for catalytic activity1 Publication
Binding sitei64 – 641Thiamine pyrophosphate2 Publications
Sitei64 – 641Important for catalytic activity1 Publication
Binding sitei114 – 1141Pyruvate2 Publications
Sitei114 – 1141Important for catalytic activity1 Publication
Metal bindingi689 – 6891Iron-sulfur(4Fe-4S) 13 Publications
Metal bindingi692 – 6921Iron-sulfur(4Fe-4S) 13 Publications
Metal bindingi695 – 6951Iron-sulfur(4Fe-4S) 13 Publications
Metal bindingi699 – 6991Iron-sulfur(4Fe-4S) 23 Publications
Metal bindingi745 – 7451Iron-sulfur(4Fe-4S) 23 Publications
Metal bindingi748 – 7481Iron-sulfur(4Fe-4S) 23 Publications
Metal bindingi751 – 7511Iron-sulfur(4Fe-4S) 23 Publications
Metal bindingi755 – 7551Iron-sulfur(4Fe-4S) 13 Publications
Metal bindingi812 – 8121Iron-sulfur(4Fe-4S) 33 Publications
Metal bindingi815 – 8151Iron-sulfur(4Fe-4S) 33 Publications
Binding sitei817 – 8171Thiamine pyrophosphate3 Publications
Metal bindingi840 – 8401Iron-sulfur(4Fe-4S) 33 Publications
Binding sitei840 – 8401Thiamine pyrophosphate3 Publications
Metal bindingi963 – 9631Magnesium3 Publications
Metal bindingi983 – 9831Calcium3 Publications
Metal bindingi985 – 9851Calcium3 Publications
Metal bindingi991 – 9911Magnesium3 Publications
Metal bindingi993 – 9931Magnesium; via carbonyl oxygen3 Publications
Sitei996 – 9961Important for catalytic activity1 Publication
Metal bindingi1056 – 10561Calcium; via carbonyl oxygen3 Publications
Metal bindingi1059 – 10591Calcium; via carbonyl oxygen3 Publications
Metal bindingi1061 – 10611Calcium; via carbonyl oxygen3 Publications
Metal bindingi1063 – 10631Calcium3 Publications
Metal bindingi1071 – 10711Iron-sulfur(4Fe-4S)2 Publications

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. iron ion binding Source: InterPro
  3. oxidoreductase activity, acting on the aldehyde or oxo group of donors Source: InterPro
  4. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. electron transport chain Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Calcium, Iron, Iron-sulfur, Magnesium, Metal-binding, Pyruvate, Thiamine pyrophosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate synthaseCurated (EC:1.2.7.11 Publication)
Alternative name(s):
Pyruvate-ferredoxin oxidoreductase.1 Publication
Short name:
POR1 Publication
Gene namesi
Name:porImported
OrganismiDesulfovibrio africanusImported
Taxonomic identifieri873 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio

Subcellular locationi

Cytoplasm 1 Publication

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12321232Pyruvate synthasePRO_0000430800Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi1195 ↔ 1212

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer.4 Publications

Protein-protein interaction databases

DIPiDIP-29036N.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B0PX-ray2.31A/B2-1232[»]
1KEKX-ray1.90A/B2-1232[»]
2C3MX-ray1.84A/B2-1232[»]
2C3OX-ray2.70A/B2-1232[»]
2C3PX-ray2.33A/B2-1232[»]
2C3UX-ray2.32A/B2-1232[»]
2C3YX-ray1.93A/B2-1232[»]
2C42X-ray1.78A/B2-1232[»]
2PDAX-ray3.00A/B2-1232[»]
2UZAX-ray2.42A/B2-1232[»]
ProteinModelPortaliP94692.
SMRiP94692. Positions 2-1232.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP94692.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini680 – 709304Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini736 – 767324Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni962 – 9654Thiamine pyrophosphate binding3 Publications
Regioni991 – 9966Thiamine pyrophosphate binding3 Publications

Sequence similaritiesi

Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 2 hits.
3.40.920.10. 1 hit.
4.10.780.10. 1 hit.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR019456. Pyrv-flavodox_OxRtase_EKR.
IPR019752. Pyrv/ketoisovalerate_OxRed_cat.
IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N.
IPR011895. Pyrv_flavodox_OxRed.
IPR002869. Pyrv_flavodox_OxRed_cen.
IPR029061. THDP-binding.
IPR011766. TPP_enzyme-bd_C.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
[Graphical view]
PfamiPF10371. EKR. 1 hit.
PF00037. Fer4. 1 hit.
PF01558. POR. 1 hit.
PF01855. POR_N. 1 hit.
PF02775. TPP_enzyme_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000159. NifJ. 1 hit.
SMARTiSM00890. EKR. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 2 hits.
SSF52922. SSF52922. 1 hit.
SSF53323. SSF53323. 1 hit.
TIGRFAMsiTIGR02176. pyruv_ox_red. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P94692-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKKMMTTDG NTATAHVAYA MSEVAAIYPI TPSSTMGEEA DDWAAQGRKN
60 70 80 90 100
IFGQTLTIRE MQSEAGAAGA VHGALAAGAL TTTFTASQGL LLMIPNMYKI
110 120 130 140 150
SGELLPGVFH VTARAIAAHA LSIFGDHQDI YAARQTGFAM LASSSVQEAH
160 170 180 190 200
DMALVAHLAA IESNVPFMHF FDGFRTSHEI QKIEVLDYAD MASLVNQKAL
210 220 230 240 250
AEFRAKSMNP EHPHVRGTAQ NPDIYFQGRE AANPYYLKVP GIVAEYMQKV
260 270 280 290 300
ASLTGRSYKL FDYVGAPDAE RVIVSMGSSC ETIEEVINHL AAKGEKIGLI
310 320 330 340 350
KVRLYRPFVS EAFFAALPAS AKVITVLDRT KEPGAPGDPL YLDVCSAFVE
360 370 380 390 400
RGEAMPKILA GRYGLGSKEF SPAMVKSVYD NMSGAKKNHF TVGIEDDVTG
410 420 430 440 450
TSLPVDNAFA DTTPKGTIQC QFWGLGADGT VGANKQAIKI IGDNTDLFAQ
460 470 480 490 500
GYFSYDSKKS GGITISHLRF GEKPIQSTYL VNRADYVACH NPAYVGIYDI
510 520 530 540 550
LEGIKDGGTF VLNSPWSSLE DMDKHLPSGI KRTIANKKLK FYNIDAVKIA
560 570 580 590 600
TDVGLGGRIN MIMQTAFFKL AGVLPFEKAV DLLKKSIHKA YGKKGEKIVK
610 620 630 640 650
MNTDAVDQAV TSLQEFKYPD SWKDAPAETK AEPMTNEFFK NVVKPILTQQ
660 670 680 690 700
GDKLPVSAFE ADGRFPLGTS QFEKRGVAIN VPQWVPENCI QCNQCAFVCP
710 720 730 740 750
HSAILPVLAK EEELVGAPAN FTALEAKGKE LKGYKFRIQI NTLDCMGCGN
760 770 780 790 800
CADICPPKEK ALVMQPLDTQ RDAQVPNLEY AARIPVKSEV LPRDSLKGSQ
810 820 830 840 850
FQEPLMEFSG ACSGCGETPY VRVITQLFGE RMFIANATGC SSIWGASAPS
860 870 880 890 900
MPYKTNRLGQ GPAWGNSLFE DAAEYGFGMN MSMFARRTHL ADLAAKALES
910 920 930 940 950
DASGDVKEAL QGWLAGKNDP IKSKEYGDKL KKLLAGQKDG LLGQIAAMSD
960 970 980 990 1000
LYTKKSVWIF GGDGWAYDIG YGGLDHVLAS GEDVNVFVMD TEVYSNTGGQ
1010 1020 1030 1040 1050
SSKATPTGAV AKFAAAGKRT GKKDLARMVM TYGYVYVATV SMGYSKQQFL
1060 1070 1080 1090 1100
KVLKEAESFP GPSLVIAYAT CINQGLRKGM GKSQDVMNTA VKSGYWPLFR
1110 1120 1130 1140 1150
YDPRLAAQGK NPFQLDSKAP DGSVEEFLMA QNRFAVLDRS FPEDAKRLRA
1160 1170 1180 1190 1200
QVAHELDVRF KELEHMAATN IFESFAPAGG KADGSVDFGE GAEFCTRDDT
1210 1220 1230
PMMARPDSGE ACDQNRAGTS EQQGDLSKRT KK
Length:1,232
Mass (Da):133,680
Last modified:May 1, 1997 - v1
Checksum:i55D84F536CCFFDBF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09702 Genomic DNA. Translation: CAA70873.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09702 Genomic DNA. Translation: CAA70873.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1B0P X-ray 2.31 A/B 2-1232 [» ]
1KEK X-ray 1.90 A/B 2-1232 [» ]
2C3M X-ray 1.84 A/B 2-1232 [» ]
2C3O X-ray 2.70 A/B 2-1232 [» ]
2C3P X-ray 2.33 A/B 2-1232 [» ]
2C3U X-ray 2.32 A/B 2-1232 [» ]
2C3Y X-ray 1.93 A/B 2-1232 [» ]
2C42 X-ray 1.78 A/B 2-1232 [» ]
2PDA X-ray 3.00 A/B 2-1232 [» ]
2UZA X-ray 2.42 A/B 2-1232 [» ]
ProteinModelPortali P94692.
SMRi P94692. Positions 2-1232.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29036N.

Chemistry

DrugBanki DB00507. Nitazoxanide.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P94692.

Family and domain databases

Gene3Di 3.40.50.920. 1 hit.
3.40.50.970. 2 hits.
3.40.920.10. 1 hit.
4.10.780.10. 1 hit.
InterProi IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR019456. Pyrv-flavodox_OxRtase_EKR.
IPR019752. Pyrv/ketoisovalerate_OxRed_cat.
IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N.
IPR011895. Pyrv_flavodox_OxRed.
IPR002869. Pyrv_flavodox_OxRed_cen.
IPR029061. THDP-binding.
IPR011766. TPP_enzyme-bd_C.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
[Graphical view ]
Pfami PF10371. EKR. 1 hit.
PF00037. Fer4. 1 hit.
PF01558. POR. 1 hit.
PF01855. POR_N. 1 hit.
PF02775. TPP_enzyme_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF000159. NifJ. 1 hit.
SMARTi SM00890. EKR. 1 hit.
[Graphical view ]
SUPFAMi SSF52518. SSF52518. 2 hits.
SSF52922. SSF52922. 1 hit.
SSF53323. SSF53323. 1 hit.
TIGRFAMsi TIGR02176. pyruv_ox_red. 1 hit.
PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and analysis of the gene encoding the pyruvate-ferredoxin oxidoreductase of Desulfovibrio africanus, production of the recombinant enzyme in Escherichia coli, and effect of carboxy-terminal deletions on its stability."
    Pieulle L., Magro V., Hatchikian E.C.
    J. Bacteriol. 179:5684-5692(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18; 304-313; 484-504 AND 1198-1216, FUNCTION.
    Strain: ATCC 19996 / DSM 2603 / NCIB 8401 / Benghazi.
  2. "Isolation and characterization of the pyruvate-ferredoxin oxidoreductase from the sulfate-reducing bacterium Desulfovibrio africanus."
    Pieulle L., Guigliarelli B., Asso M., Dole F., Bernadac A., Hatchikian E.C.
    Biochim. Biophys. Acta 1250:49-59(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.
    Strain: ATCC 19996 / DSM 2603 / NCIB 8401 / Benghazi.
  3. "Crystal structures of the key anaerobic enzyme pyruvate:ferredoxin oxidoreductase, free and in complex with pyruvate."
    Chabriere E., Charon M.H., Volbeda A., Pieulle L., Hatchikian E.C., Fontecilla-Camps J.C.
    Nat. Struct. Biol. 6:182-190(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS) OF 2-1232 IN COMPLEX WITH IRON-SULFUR(4FE-4S); CALCIUM ION; MAGNESIUM ION; PYRUVATE AND THIAMINEPYROPHOSPHATE, SUBUNIT, DISULFIDE BOND.
  4. "Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase."
    Chabriere E., Vernede X., Guigliarelli B., Charon M.H., Hatchikian E.C., Fontecilla-Camps J.C.
    Science 294:2559-2563(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 2-1232 IN COMPLEX WITH CALCIUM; IRON-SULFUR(4FE-4S); MAGNESIUM AND THIAMINEPYROPHOSPHATE ANALOG, SUBUNIT.
  5. "Flexibility of thiamine diphosphate revealed by kinetic crystallographic studies of the reaction of pyruvate-ferredoxin oxidoreductase with pyruvate."
    Cavazza C., Contreras-Martel C., Pieulle L., Chabriere E., Hatchikian E.C., Fontecilla-Camps J.C.
    Structure 14:217-224(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 2-1232 IN COMPLEX WITH IRON-SULFUR(4FE-4S); CALCIUM ION; MAGNESIUM ION; PYRUVATE AND THIAMINEPYROPHOSPHATE, SUBUNIT.

Entry informationi

Entry nameiPOR_DESAF
AccessioniPrimary (citable) accession number: P94692
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 29, 2014
Last sequence update: May 1, 1997
Last modified: October 29, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3