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P94692

- P94692_DESAF

UniProt

P94692 - P94692_DESAF

Protein

Pyruvate-flavodoxin oxidoreductase

Gene

por

Organism
Desulfovibrio africanus
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Oxidoreductase required for the transfer of electrons from pyruvate to flavodoxin.UniRule annotation

    Catalytic activityi

    Pyruvate + CoA + oxidized flavodoxin = acetyl-CoA + CO2 + reduced flavodoxin.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei31 – 311Pyruvate 1Imported
    Binding sitei31 – 311Pyruvate 2Imported
    Sitei31 – 311Important for catalytic activityImported
    Binding sitei64 – 641ThiaminepyrophosphateImported
    Sitei64 – 641Important for catalytic activityImported
    Binding sitei88 – 881ThiaminepyrophosphateImported
    Binding sitei114 – 1141Pyruvate 1Imported
    Binding sitei114 – 1141Pyruvate 2Imported
    Sitei114 – 1141Important for catalytic activityImported
    Metal bindingi689 – 6891Iron-sulfur(4Fe-4S) 3Imported
    Metal bindingi690 – 6901Iron-sulfur(4Fe-4S) 3; via amide nitrogenImported
    Metal bindingi692 – 6921Iron-sulfur(4Fe-4S) 3Imported
    Metal bindingi693 – 6931Iron-sulfur(4Fe-4S) 3; via amide nitrogenImported
    Metal bindingi695 – 6951Iron-sulfur(4Fe-4S) 3Imported
    Metal bindingi699 – 6991Iron-sulfur(4Fe-4S) 2Imported
    Metal bindingi745 – 7451Iron-sulfur(4Fe-4S) 2Imported
    Metal bindingi748 – 7481Iron-sulfur(4Fe-4S) 2Imported
    Metal bindingi749 – 7491Iron-sulfur(4Fe-4S) 2; via amide nitrogenImported
    Metal bindingi751 – 7511Iron-sulfur(4Fe-4S) 2Imported
    Metal bindingi755 – 7551Iron-sulfur(4Fe-4S) 3Imported
    Metal bindingi761 – 7611Iron-sulfur(4Fe-4S) 3Imported
    Metal bindingi812 – 8121Iron-sulfur(4Fe-4S) 1Imported
    Metal bindingi815 – 8151Iron-sulfur(4Fe-4S) 1Imported
    Binding sitei817 – 8171ThiaminepyrophosphateImported
    Metal bindingi840 – 8401Iron-sulfur(4Fe-4S) 1Imported
    Binding sitei840 – 8401ThiaminepyrophosphateImported
    Binding sitei869 – 8691ThiaminepyrophosphateImported
    Metal bindingi963 – 9631MagnesiumImported
    Metal bindingi983 – 9831CalciumImported
    Metal bindingi985 – 9851CalciumImported
    Metal bindingi991 – 9911MagnesiumImported
    Metal bindingi993 – 9931MagnesiumImported
    Binding sitei996 – 9961Pyruvate 1Imported
    Sitei996 – 9961Important for catalytic activityImported
    Metal bindingi1056 – 10561Calcium; via carbonyl oxygenImported
    Metal bindingi1057 – 10571Calcium; via carbonyl oxygenImported
    Metal bindingi1059 – 10591Calcium; via carbonyl oxygenImported
    Metal bindingi1061 – 10611Calcium; via carbonyl oxygenImported
    Metal bindingi1063 – 10631CalciumImported
    Metal bindingi1071 – 10711Iron-sulfur(4Fe-4S) 1Imported
    Binding sitei1202 – 12021Pyruvate 1Imported

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. iron ion binding Source: InterPro
    3. oxidoreductase activity, acting on the aldehyde or oxo group of donors Source: InterPro
    4. thiamine pyrophosphate binding Source: InterPro

    GO - Biological processi

    1. electron transport chain Source: InterPro

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Electron transportUniRule annotation, Transport

    Keywords - Ligandi

    4Fe-4SUniRule annotation, CalciumImported, Iron, Iron-sulfur, MagnesiumImported, Metal-binding, PyruvateImported

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate-flavodoxin oxidoreductaseUniRule annotation (EC:1.2.7.-UniRule annotation)
    Gene namesi
    Name:porImported
    OrganismiDesulfovibrio africanusImported
    Taxonomic identifieri873 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio

    Pathology & Biotechi

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-29036N.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1B0PX-ray2.31A/B2-1232[»]
    1KEKX-ray1.90A/B2-1232[»]
    2C3MX-ray1.84A/B2-1232[»]
    2C3OX-ray2.70A/B2-1232[»]
    2C3PX-ray2.33A/B2-1232[»]
    2C3UX-ray2.32A/B2-1232[»]
    2C3YX-ray1.93A/B2-1232[»]
    2C42X-ray1.78A/B2-1232[»]
    2PDAX-ray3.00A/B2-1232[»]
    2UZAX-ray2.42A/B2-1232[»]
    ProteinModelPortaliP94692.
    SMRiP94692. Positions 2-1232.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP94692.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni962 – 9654Thiaminepyrophosphate bindingImported
    Regioni991 – 9966Thiaminepyrophosphate bindingImported

    Sequence similaritiesi

    Belongs to the nifJ family.UniRule annotation

    Family and domain databases

    Gene3Di3.40.50.920. 1 hit.
    3.40.50.970. 2 hits.
    3.40.920.10. 1 hit.
    4.10.780.10. 1 hit.
    InterProiIPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR019456. Pyrv-flavodox_OxRtase_EKR.
    IPR019752. Pyrv/ketoisovalerate_OxRed_cat.
    IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N.
    IPR011895. Pyrv_flavodox_OxRed.
    IPR002869. Pyrv_flavodox_OxRed_cen.
    IPR029061. THDP-binding.
    IPR011766. TPP_enzyme-bd_C.
    IPR009014. Transketo_C/Pyr-ferredox_oxred.
    [Graphical view]
    PfamiPF10371. EKR. 1 hit.
    PF00037. Fer4. 1 hit.
    PF01558. POR. 1 hit.
    PF01855. POR_N. 1 hit.
    PF02775. TPP_enzyme_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000159. NifJ. 1 hit.
    SMARTiSM00890. EKR. 1 hit.
    [Graphical view]
    SUPFAMiSSF52518. SSF52518. 2 hits.
    SSF52922. SSF52922. 1 hit.
    SSF53323. SSF53323. 1 hit.
    TIGRFAMsiTIGR02176. pyruv_ox_red. 1 hit.
    PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P94692-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKKMMTTDG NTATAHVAYA MSEVAAIYPI TPSSTMGEEA DDWAAQGRKN     50
    IFGQTLTIRE MQSEAGAAGA VHGALAAGAL TTTFTASQGL LLMIPNMYKI 100
    SGELLPGVFH VTARAIAAHA LSIFGDHQDI YAARQTGFAM LASSSVQEAH 150
    DMALVAHLAA IESNVPFMHF FDGFRTSHEI QKIEVLDYAD MASLVNQKAL 200
    AEFRAKSMNP EHPHVRGTAQ NPDIYFQGRE AANPYYLKVP GIVAEYMQKV 250
    ASLTGRSYKL FDYVGAPDAE RVIVSMGSSC ETIEEVINHL AAKGEKIGLI 300
    KVRLYRPFVS EAFFAALPAS AKVITVLDRT KEPGAPGDPL YLDVCSAFVE 350
    RGEAMPKILA GRYGLGSKEF SPAMVKSVYD NMSGAKKNHF TVGIEDDVTG 400
    TSLPVDNAFA DTTPKGTIQC QFWGLGADGT VGANKQAIKI IGDNTDLFAQ 450
    GYFSYDSKKS GGITISHLRF GEKPIQSTYL VNRADYVACH NPAYVGIYDI 500
    LEGIKDGGTF VLNSPWSSLE DMDKHLPSGI KRTIANKKLK FYNIDAVKIA 550
    TDVGLGGRIN MIMQTAFFKL AGVLPFEKAV DLLKKSIHKA YGKKGEKIVK 600
    MNTDAVDQAV TSLQEFKYPD SWKDAPAETK AEPMTNEFFK NVVKPILTQQ 650
    GDKLPVSAFE ADGRFPLGTS QFEKRGVAIN VPQWVPENCI QCNQCAFVCP 700
    HSAILPVLAK EEELVGAPAN FTALEAKGKE LKGYKFRIQI NTLDCMGCGN 750
    CADICPPKEK ALVMQPLDTQ RDAQVPNLEY AARIPVKSEV LPRDSLKGSQ 800
    FQEPLMEFSG ACSGCGETPY VRVITQLFGE RMFIANATGC SSIWGASAPS 850
    MPYKTNRLGQ GPAWGNSLFE DAAEYGFGMN MSMFARRTHL ADLAAKALES 900
    DASGDVKEAL QGWLAGKNDP IKSKEYGDKL KKLLAGQKDG LLGQIAAMSD 950
    LYTKKSVWIF GGDGWAYDIG YGGLDHVLAS GEDVNVFVMD TEVYSNTGGQ 1000
    SSKATPTGAV AKFAAAGKRT GKKDLARMVM TYGYVYVATV SMGYSKQQFL 1050
    KVLKEAESFP GPSLVIAYAT CINQGLRKGM GKSQDVMNTA VKSGYWPLFR 1100
    YDPRLAAQGK NPFQLDSKAP DGSVEEFLMA QNRFAVLDRS FPEDAKRLRA 1150
    QVAHELDVRF KELEHMAATN IFESFAPAGG KADGSVDFGE GAEFCTRDDT 1200
    PMMARPDSGE ACDQNRAGTS EQQGDLSKRT KK 1232
    Length:1,232
    Mass (Da):133,680
    Last modified:May 1, 1997 - v1
    Checksum:i55D84F536CCFFDBF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09702 Genomic DNA. Translation: CAA70873.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09702 Genomic DNA. Translation: CAA70873.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1B0P X-ray 2.31 A/B 2-1232 [» ]
    1KEK X-ray 1.90 A/B 2-1232 [» ]
    2C3M X-ray 1.84 A/B 2-1232 [» ]
    2C3O X-ray 2.70 A/B 2-1232 [» ]
    2C3P X-ray 2.33 A/B 2-1232 [» ]
    2C3U X-ray 2.32 A/B 2-1232 [» ]
    2C3Y X-ray 1.93 A/B 2-1232 [» ]
    2C42 X-ray 1.78 A/B 2-1232 [» ]
    2PDA X-ray 3.00 A/B 2-1232 [» ]
    2UZA X-ray 2.42 A/B 2-1232 [» ]
    ProteinModelPortali P94692.
    SMRi P94692. Positions 2-1232.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29036N.

    Chemistry

    DrugBanki DB00507. Nitazoxanide.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P94692.

    Family and domain databases

    Gene3Di 3.40.50.920. 1 hit.
    3.40.50.970. 2 hits.
    3.40.920.10. 1 hit.
    4.10.780.10. 1 hit.
    InterProi IPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR019456. Pyrv-flavodox_OxRtase_EKR.
    IPR019752. Pyrv/ketoisovalerate_OxRed_cat.
    IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N.
    IPR011895. Pyrv_flavodox_OxRed.
    IPR002869. Pyrv_flavodox_OxRed_cen.
    IPR029061. THDP-binding.
    IPR011766. TPP_enzyme-bd_C.
    IPR009014. Transketo_C/Pyr-ferredox_oxred.
    [Graphical view ]
    Pfami PF10371. EKR. 1 hit.
    PF00037. Fer4. 1 hit.
    PF01558. POR. 1 hit.
    PF01855. POR_N. 1 hit.
    PF02775. TPP_enzyme_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000159. NifJ. 1 hit.
    SMARTi SM00890. EKR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52518. SSF52518. 2 hits.
    SSF52922. SSF52922. 1 hit.
    SSF53323. SSF53323. 1 hit.
    TIGRFAMsi TIGR02176. pyruv_ox_red. 1 hit.
    PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Claude H.
      Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: NCIB 8401Imported.
    2. "Isolation and analysis of the gene encoding the pyruvate-ferredoxin oxidoreductase of Desulfovibrio africanus, production of the recombinant enzyme in Escherichia coli, and effect of carboxy-terminal deletions on its stability."
      Pieulle L., Magro V., Hatchikian E.C.
      J. Bacteriol. 179:5684-5692(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: NCIB 8401Imported.
    3. "Crystal structures of the key anaerobic enzyme pyruvate:ferredoxin oxidoreductase, free and in complex with pyruvate."
      Chabriere E., Charon M.H., Volbeda A., Pieulle L., Hatchikian E.C., Fontecilla-Camps J.C.
      Nat. Struct. Biol. 6:182-190(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS) OF 2-1232 IN COMPLEX WITH CALCIUM; IRON-SULFUR(4FE-4S); MAGNESIUM; PYRUVATE AND THIAMINEPYROPHOSPHATE, ACTIVE SITE.
    4. "Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase."
      Chabriere E., Vernede X., Guigliarelli B., Charon M.H., Hatchikian E.C., Fontecilla-Camps J.C.
      Science 294:2559-2563(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 2-1232 IN COMPLEX WITH CALCIUM; IRON-SULFUR(4FE-4S) AND MAGNESIUM.
    5. "Flexibility of thiamine diphosphate revealed by kinetic crystallographic studies of the reaction of pyruvate-ferredoxin oxidoreductase with pyruvate."
      Cavazza C., Contreras-Martel C., Pieulle L., Chabriere E., Hatchikian E.C., Fontecilla-Camps J.C.
      Structure 14:217-224(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 2-1232 IN COMPLEX WITH CALCIUM; IRON-SULFUR(4FE-4S); MAGNESIUM; PYRUVATE AND THIAMINEPYROPHOSPHATE.
    6. "Pyruvate:Ferredoxin Oxidoreductase."
      Chabriere E., Cavazza C., Contreras-Martel C., Fontecilla-Camps J.C.
      Submitted (APR-2007) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.42 ANGSTROMS) OF 2-1232 IN COMPLEX WITH CALCIUM; IRON-SULFUR(4FE-4S) AND MAGNESIUM.

    Entry informationi

    Entry nameiP94692_DESAF
    AccessioniPrimary (citable) accession number: P94692
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 1, 1997
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3