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Protein

Acidic cytochrome c3

Gene
N/A
Organism
Desulfovibrio africanus
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exchanges electrons specifically with the basic cytochrome c3.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron (heme 1 axial ligand)
Metal bindingi51 – 511Iron (heme 3 axial ligand)
Binding sitei60 – 601Heme 1 (covalent)
Binding sitei63 – 631Heme 1 (covalent)
Metal bindingi64 – 641Iron (heme 1 axial ligand)
Metal bindingi65 – 651Iron (heme 2 axial ligand)
Binding sitei83 – 831Heme 2 (covalent)
Binding sitei86 – 861Heme 2 (covalent)
Metal bindingi87 – 871Iron (heme 2 axial ligand)
Metal bindingi103 – 1031Iron (heme 4 axial ligand)
Binding sitei106 – 1061Heme 3 (covalent)
Binding sitei109 – 1091Heme 3 (covalent)
Metal bindingi110 – 1101Iron (heme 3 axial ligand)
Binding sitei120 – 1201Heme 4 (covalent)
Binding sitei123 – 1231Heme 4 (covalent)
Metal bindingi124 – 1241Iron (heme 4 axial ligand)

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Sulfate respiration, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic cytochrome c3
OrganismiDesulfovibrio africanus
Taxonomic identifieri873 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Add
BLAST
Chaini25 – 127103Acidic cytochrome c3PRO_0000006502Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei25 – 251Pyrrolidone carboxylic acid

Post-translational modificationi

Binds 4 heme groups per subunit.
The N-terminus is blocked.

Keywords - PTMi

Pyrrolidone carboxylic acid

Interactioni

Protein-protein interaction databases

STRINGi690850.Desaf_0450.

Structurei

Secondary structure

1
127
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi34 – 363Combined sources
Helixi48 – 558Combined sources
Helixi60 – 623Combined sources
Helixi83 – 853Combined sources
Helixi98 – 11316Combined sources
Helixi120 – 1234Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CAOX-ray1.60A26-127[»]
3CARX-ray1.90A26-127[»]
ProteinModelPortaliP94690.
SMRiP94690. Positions 26-127.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP94690.

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG41062QK. Bacteria.
ENOG4112BW6. LUCA.

Family and domain databases

InterProiIPR002322. Cyt_c_III.
IPR020942. Cyt_c_III_dom.
IPR011031. Multihaem_cyt.
[Graphical view]
PfamiPF02085. Cytochrom_CIII. 1 hit.
[Graphical view]
PRINTSiPR00609. CYTOCHROMEC3.
SUPFAMiSSF48695. SSF48695. 1 hit.
PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P94690-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKHTLIALT LLAAATLFSL PAFSQEDMTH VPTDAFGKLE RPAAVFNHDE
60 70 80 90 100
HNEKAGIESC NACHHVWVNG VLAEDEDSVG TPCSDCHALE QDGDTPGLQD
110 120
AYHQQCWGCH EKQAKGPVMC GECHVKN
Length:127
Mass (Da):13,856
Last modified:May 1, 1997 - v1
Checksum:iDE6E3B16FA4BF8B3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti67 – 671W → P AA sequence (PubMed:8573595).Curated

Mass spectrometryi

Molecular mass is 13742±1.2 Da from positions 25 - 117. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09718 Genomic DNA. Translation: CAA70884.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09718 Genomic DNA. Translation: CAA70884.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CAOX-ray1.60A26-127[»]
3CARX-ray1.90A26-127[»]
ProteinModelPortaliP94690.
SMRiP94690. Positions 26-127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi690850.Desaf_0450.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG41062QK. Bacteria.
ENOG4112BW6. LUCA.

Miscellaneous databases

EvolutionaryTraceiP94690.

Family and domain databases

InterProiIPR002322. Cyt_c_III.
IPR020942. Cyt_c_III_dom.
IPR011031. Multihaem_cyt.
[Graphical view]
PfamiPF02085. Cytochrom_CIII. 1 hit.
[Graphical view]
PRINTSiPR00609. CYTOCHROMEC3.
SUPFAMiSSF48695. SSF48695. 1 hit.
PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Further characterization of the two tetraheme cytochromes c3 from Desulfovibiro africanus: nucleotide sequences, EPR spectroscopy and biological activity."
    Magro V., Pieulle L., Forget N., Guigliarelli B., Petillot Y., Hatchikian E.C.
    Biochim. Biophys. Acta 1342:149-163(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION, MASS SPECTROMETRY.
    Strain: ATCC 19996 / DSM 2603 / NCIB 8401 / Benghazi.
  2. "Biochemical studies of the c-type cytochromes of the sulfate reducer Desulfovibrio africanus. Characterization of two tetraheme cytochromes c3 with different specificity."
    Pieulle L., Haladjian J., Bonicel J., Hatchikian E.C.
    Biochim. Biophys. Acta 1273:51-61(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 29-68, FUNCTION, HEME-BINDING, BLOCKAGE OF N-TERMINUS.
    Strain: ATCC 19996 / DSM 2603 / NCIB 8401 / Benghazi.
  3. "Crystal structure of the oxidised and reduced acidic cytochrome c3 from Desulfovibrio africanus."
    Noerager S., Legrand P., Pieulle L., Hatchikian C., Roth M.
    J. Mol. Biol. 290:881-902(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 25-127.

Entry informationi

Entry nameiCYC3A_DESAF
AccessioniPrimary (citable) accession number: P94690
Secondary accession number(s): Q9R4E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 1997
Last modified: January 20, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.