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Protein

Toluene-4-sulfonate monooxygenase system reductase subunit TsaB1

Gene

tsaB1

Organism
Comamonas testosteroni (Pseudomonas testosteroni)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Iron-sulfur flavoprotein carrying electrons from NADH to the oxygenase TsaM. Involved in the toluene-4-sulfonate degradation pathway.1 Publication

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi266Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi271Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi274Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi304Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi110 – 220NADBy similarityAdd BLAST111

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism, Electron transport, Transport

Keywords - Ligandi

2Fe-2S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-5161.

Names & Taxonomyi

Protein namesi
Recommended name:
Toluene-4-sulfonate monooxygenase system reductase subunit TsaB1 (EC:1.5.1.-)
Alternative name(s):
TS methylmonooxygenase system, reductase B
Toluenesulfonate methyl-monooxygenase reductase component TsaB1
Gene namesi
Name:tsaB1
Synonyms:tsaB
Encoded oniPlasmid pTSA0 Publication
OrganismiComamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifieri285 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeComamonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004191211 – 317Toluene-4-sulfonate monooxygenase system reductase subunit TsaB1Add BLAST317

Interactioni

Subunit structurei

Monomer. Part of the p-toluenesulfonate methyl-monooxygenase complex TsaBM, comprising the reductase TsaB and the oxygenase TsaM.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP94680.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 108FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST107
Domaini230 – 3172Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST88

Sequence similaritiesi

Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK15769.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000951. Ph_dOase_redase.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00409. PHDIOXRDTASE.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P94680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSADVPVTVA AVRAVARDVL ALELRHANGQ PLPGASAGAH IDLALPNGLV
60 70 80 90 100
RQYSLVNATG QATMDCYQVA VGWDANSRGG SVWIHEKLKV GQALRVTHRA
110 120 130 140 150
TCSEMAPEHR RVLLLAGGIG VTPIYAMAQA CAQQGVDVEL WASARSAPRL
160 170 180 190 200
AYLEELKALL GQRLHLHADD EQGGPMNLTE RLATQRWDAV YACGPAPMLD
210 220 230 240 250
ALTAATAHWA PGSVRMERFK GAEQPASERQ PFELVLQRAG LSTTVDAHES
260 270 280 290 300
VLDAMERVGV DFPWSCREGI CGTCEAPVLE GEVQHLDYVL SPEERAEQRR
310
MMVCVSRCGG GRLVLDI
Length:317
Mass (Da):34,186
Last modified:May 1, 1997 - v1
Checksum:iEB21C7CE82CF965E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF311437 Genomic DNA. Translation: AAC44805.1.

Genome annotation databases

KEGGiag:AAC44805.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF311437 Genomic DNA. Translation: AAC44805.1.

3D structure databases

ProteinModelPortaliP94680.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAC44805.

Phylogenomic databases

KOiK15769.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-5161.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000951. Ph_dOase_redase.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00409. PHDIOXRDTASE.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSAB1_COMTE
AccessioniPrimary (citable) accession number: P94680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: May 1, 1997
Last modified: September 7, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.