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Protein

HTH-type transcriptional regulator TsaR

Gene

tsaR

Organism
Comamonas testosteroni (Pseudomonas testosteroni)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Regulates expression of the tsaMBCD1 operon and of tsaT in response to p-toluenesulfonate (TSA). Acts by binding directly to the promoter region. Binding to the tsa promoter depends on TSA concentration.2 Publications

Enzyme regulationi

Sensitive to oxygen.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi18 – 37H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator TsaR
Gene namesi
Name:tsaR
Encoded oniPlasmid pTSA0 Publication
OrganismiComamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifieri285 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeComamonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004303011 – 298HTH-type transcriptional regulator TsaRAdd BLAST298

Interactioni

Subunit structurei

Homotetramer. Dimer of dimers related by a twofold axis.1 Publication

Structurei

Secondary structure

1298
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 15Combined sources14
Helixi18 – 24Combined sources7
Helixi29 – 43Combined sources15
Beta strandi47 – 50Combined sources4
Beta strandi52 – 57Combined sources6
Helixi59 – 90Combined sources32
Beta strandi92 – 97Combined sources6
Helixi99 – 103Combined sources5
Helixi105 – 116Combined sources12
Beta strandi121 – 126Combined sources6
Turni129 – 132Combined sources4
Helixi133 – 138Combined sources6
Beta strandi139 – 147Combined sources9
Helixi150 – 152Combined sources3
Beta strandi157 – 164Combined sources8
Beta strandi167 – 172Combined sources6
Turni176 – 179Combined sources4
Helixi183 – 186Combined sources4
Beta strandi189 – 194Combined sources6
Helixi202 – 210Combined sources9
Beta strandi217 – 222Combined sources6
Turni225 – 227Combined sources3
Helixi228 – 233Combined sources6
Beta strandi238 – 242Combined sources5
Helixi243 – 247Combined sources5
Helixi252 – 254Combined sources3
Beta strandi255 – 257Combined sources3
Beta strandi268 – 274Combined sources7
Helixi281 – 293Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FXQX-ray1.85A/B1-298[»]
3FXRX-ray2.50A/B1-298[»]
3FXUX-ray1.95A/B1-298[»]
3FZJX-ray7.10A/B/C/D/E/F/G/H/I/J1-298[»]
3N6TX-ray1.85A91-295[»]
3N6UX-ray1.87A91-295[»]
ProteinModelPortaliP94678.
SMRiP94678.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP94678.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 58HTH lysR-typePROSITE-ProRule annotationAdd BLAST58

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 100Toluene-4-sulfonate binding3
Regioni195 – 200Important for toluene-4-sulfonate binding6

Sequence similaritiesi

Contains 1 HTH lysR-type DNA-binding domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P94678-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLQTLQALI CIEEVGSLRA AAQLLHLSQP ALSAAIQQLE DELKAPLLVR
60 70 80 90 100
TKRGVSLTSF GQAFMKHARL IVTESRRAQE EIGQLRGRWE GHITFAASPA
110 120 130 140 150
IALAALPLAL ASFAREFPDV TVNVRDGMYP AVSPQLRDGT LDFALTAAHK
160 170 180 190 200
HDIDTDLEAQ PLYVSDVVIV GQRQHPMANA TRLAELQECR WAFSSAPRGP
210 220 230 240 250
GAIIRNAFAR YGLPEPKLGL VCESFLALPG VVAHSDLLTT MPRTLYERNA
260 270 280 290
FKDQLCSIPL QDALPNPTIY VLRRHDLPVT PAAAGLIRWI QHHALQTG
Length:298
Mass (Da):32,663
Last modified:May 1, 1997 - v1
Checksum:i4740B8B80DED0C22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF311437 Genomic DNA. Translation: AAC44806.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF311437 Genomic DNA. Translation: AAC44806.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FXQX-ray1.85A/B1-298[»]
3FXRX-ray2.50A/B1-298[»]
3FXUX-ray1.95A/B1-298[»]
3FZJX-ray7.10A/B/C/D/E/F/G/H/I/J1-298[»]
3N6TX-ray1.85A91-295[»]
3N6UX-ray1.87A91-295[»]
ProteinModelPortaliP94678.
SMRiP94678.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP94678.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSAR_COMTE
AccessioniPrimary (citable) accession number: P94678
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 2014
Last sequence update: May 1, 1997
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.