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Protein

HTH-type transcriptional regulator TsaR

Gene

tsaR

Organism
Comamonas testosteroni (Pseudomonas testosteroni)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Regulates expression of the tsaMBCD1 operon and of tsaT in response to p-toluenesulfonate (TSA). Acts by binding directly to the promoter region. Binding to the tsa promoter depends on TSA concentration.2 Publications

Enzyme regulationi

Sensitive to oxygen.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi18 – 3720H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator TsaR
Gene namesi
Name:tsaR
Encoded oniPlasmid pTSA0 Publication
OrganismiComamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifieri285 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeComamonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298HTH-type transcriptional regulator TsaRPRO_0000430301Add
BLAST

Interactioni

Subunit structurei

Homotetramer. Dimer of dimers related by a twofold axis.1 Publication

Structurei

Secondary structure

1
298
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1514Combined sources
Helixi18 – 247Combined sources
Helixi29 – 4315Combined sources
Beta strandi47 – 504Combined sources
Beta strandi52 – 576Combined sources
Helixi59 – 9032Combined sources
Beta strandi92 – 976Combined sources
Helixi99 – 1035Combined sources
Helixi105 – 11612Combined sources
Beta strandi121 – 1266Combined sources
Turni129 – 1324Combined sources
Helixi133 – 1386Combined sources
Beta strandi139 – 1479Combined sources
Helixi150 – 1523Combined sources
Beta strandi157 – 1648Combined sources
Beta strandi167 – 1726Combined sources
Turni176 – 1794Combined sources
Helixi183 – 1864Combined sources
Beta strandi189 – 1946Combined sources
Helixi202 – 2109Combined sources
Beta strandi217 – 2226Combined sources
Turni225 – 2273Combined sources
Helixi228 – 2336Combined sources
Beta strandi238 – 2425Combined sources
Helixi243 – 2475Combined sources
Helixi252 – 2543Combined sources
Beta strandi255 – 2573Combined sources
Beta strandi268 – 2747Combined sources
Helixi281 – 29313Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FXQX-ray1.85A/B1-298[»]
3FXRX-ray2.50A/B1-298[»]
3FXUX-ray1.95A/B1-298[»]
3FZJX-ray7.10A/B/C/D/E/F/G/H/I/J1-298[»]
3N6TX-ray1.85A91-295[»]
3N6UX-ray1.87A91-295[»]
ProteinModelPortaliP94678.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP94678.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5858HTH lysR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni98 – 1003Toluene-4-sulfonate binding
Regioni195 – 2006Important for toluene-4-sulfonate binding

Sequence similaritiesi

Contains 1 HTH lysR-type DNA-binding domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P94678-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLQTLQALI CIEEVGSLRA AAQLLHLSQP ALSAAIQQLE DELKAPLLVR
60 70 80 90 100
TKRGVSLTSF GQAFMKHARL IVTESRRAQE EIGQLRGRWE GHITFAASPA
110 120 130 140 150
IALAALPLAL ASFAREFPDV TVNVRDGMYP AVSPQLRDGT LDFALTAAHK
160 170 180 190 200
HDIDTDLEAQ PLYVSDVVIV GQRQHPMANA TRLAELQECR WAFSSAPRGP
210 220 230 240 250
GAIIRNAFAR YGLPEPKLGL VCESFLALPG VVAHSDLLTT MPRTLYERNA
260 270 280 290
FKDQLCSIPL QDALPNPTIY VLRRHDLPVT PAAAGLIRWI QHHALQTG
Length:298
Mass (Da):32,663
Last modified:May 1, 1997 - v1
Checksum:i4740B8B80DED0C22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF311437 Genomic DNA. Translation: AAC44806.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF311437 Genomic DNA. Translation: AAC44806.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FXQX-ray1.85A/B1-298[»]
3FXRX-ray2.50A/B1-298[»]
3FXUX-ray1.95A/B1-298[»]
3FZJX-ray7.10A/B/C/D/E/F/G/H/I/J1-298[»]
3N6TX-ray1.85A91-295[»]
3N6UX-ray1.87A91-295[»]
ProteinModelPortaliP94678.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP94678.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSAR_COMTE
AccessioniPrimary (citable) accession number: P94678
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 2014
Last sequence update: May 1, 1997
Last modified: December 9, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.