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Protein

3-isopropylmalate dehydrogenase

Gene

leuB

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate (By similarity).By similarity

Catalytic activityi

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: L-leucine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase large subunit (leuC), 3-isopropylmalate dehydratase small subunit (leuD)
  3. 3-isopropylmalate dehydrogenase (leuB)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881SubstrateBy similarity
Binding sitei98 – 981SubstrateBy similarity
Binding sitei122 – 1221SubstrateBy similarity
Sitei129 – 1291Important for catalysisBy similarity
Sitei179 – 1791Important for catalysisBy similarity
Metal bindingi212 – 2121Magnesium or manganeseBy similarity
Binding sitei212 – 2121SubstrateBy similarity
Metal bindingi236 – 2361Magnesium or manganeseBy similarity
Metal bindingi240 – 2401Magnesium or manganeseBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi272 – 28413NADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

UniPathwayiUPA00048; UER00072.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydrogenase (EC:1.1.1.85)
Alternative name(s):
3-IPM-DH
Beta-IPM dehydrogenase
Short name:
IMDH
Gene namesi
Name:leuB
Ordered Locus Names:Cgl1286, cg1453
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3403403-isopropylmalate dehydrogenasePRO_0000083797Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi196627.cg1453.

Structurei

3D structure databases

ProteinModelPortaliP94631.
SMRiP94631. Positions 2-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C0C. Bacteria.
COG0473. LUCA.
KOiK00052.
OMAiLGARRWH.
OrthoDBiEOG65N1BN.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_01035. LeuB_type2.
InterProiIPR023698. 3-isopropylmalate_DH_LeuB.
IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P94631-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLAVIGGDG IGPEVTAEAL KVLNAVRDDI ETTDYDLGAR RYLKNGELLT
60 70 80 90 100
DEDLASLREH DAILLGAIGA PGSVPPGILE RGLLLKMRFA LDHHVNLRPS
110 120 130 140 150
KLYDGVESPL RNPGKIDFVV VREGTEGAYT GNGGAIRVGT PHEIANETSV
160 170 180 190 200
NTRYGAERVI RYAFELAQSR RKKLTLVHKT NVLVHGGGLW QRTVDEVAKE
210 220 230 240 250
YPEVAVDYNH IDAATIYLVT DPSRFDVIVT DNLFGDILTD EAGAVSGGIG
260 270 280 290 300
LAASGNIDAT GTNPSMFEPV HGSAPDIAGQ GIADPTAAIL SAAMLLRHLG
310 320 330 340
DEDNAVRIET AIAADVAGRD NSQPISTTEV GDRIVKALQS
Length:340
Mass (Da):36,145
Last modified:May 1, 1997 - v1
Checksum:iE53D77F5F45D6958
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09578 Genomic DNA. Translation: CAA70767.1.
BA000036 Genomic DNA. Translation: BAB98679.1.
BX927151 Genomic DNA. Translation: CAF19988.1.
RefSeqiNP_600508.1. NC_003450.3.
WP_011014258.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB98679; BAB98679; BAB98679.
CAF19988; CAF19988; cg1453.
GeneIDi1019267.
KEGGicgb:cg1453.
cgl:NCgl1237.
PATRICi21494614. VBICorGlu203724_1263.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09578 Genomic DNA. Translation: CAA70767.1.
BA000036 Genomic DNA. Translation: BAB98679.1.
BX927151 Genomic DNA. Translation: CAF19988.1.
RefSeqiNP_600508.1. NC_003450.3.
WP_011014258.1. NC_006958.1.

3D structure databases

ProteinModelPortaliP94631.
SMRiP94631. Positions 2-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg1453.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB98679; BAB98679; BAB98679.
CAF19988; CAF19988; cg1453.
GeneIDi1019267.
KEGGicgb:cg1453.
cgl:NCgl1237.
PATRICi21494614. VBICorGlu203724_1263.

Phylogenomic databases

eggNOGiENOG4105C0C. Bacteria.
COG0473. LUCA.
KOiK00052.
OMAiLGARRWH.
OrthoDBiEOG65N1BN.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00072.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_01035. LeuB_type2.
InterProiIPR023698. 3-isopropylmalate_DH_LeuB.
IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiLEU3_CORGL
AccessioniPrimary (citable) accession number: P94631
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: May 11, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.