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Protein
Submitted name:

Endo-1,4-beta glucanase EngF

Gene

engF

Organism
Clostridium cellulovorans
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi391 – 3911Calcium; via carbonyl oxygenCombined sources
Metal bindingi393 – 3931CalciumCombined sources
Metal bindingi418 – 4181Calcium; via carbonyl oxygenCombined sources
Metal bindingi551 – 5511CalciumCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotationImported, Hydrolase

Keywords - Ligandi

CalciumCombined sources, Metal-bindingCombined sources

Protein family/group databases

CAZyiCBM17. Carbohydrate-Binding Module Family 17.
GH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Submitted name:
Endo-1,4-beta glucanase EngFImported (EC:3.2.1.4Imported)
Gene namesi
Name:engFImported
OrganismiClostridium cellulovoransImported
Taxonomic identifieri1493 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Chaini30 – 557528Sequence analysisPRO_5004161638Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi573061.Clocel_3662.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J83X-ray1.70A/B378-557[»]
1J84X-ray2.02A378-557[»]
ProteinModelPortaliP94622.
SMRiP94622. Positions 38-374, 378-557.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP94622.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini64 – 326263CellulaseInterPro annotationAdd
BLAST
Domaini346 – 556211CBM_17_28InterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 5 (cellulase A) family.UniRule annotation

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

eggNOGiENOG4107QWR. Bacteria.
COG2730. LUCA.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR005086. CBM_fam_17/28.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF03424. CBM_17_28. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P94622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNNVKKKIL SIVAAGAMLM ALVPNINVAA ETTYSNLTGN ANVKKPSVGG
60 70 80 90 100
KLQLLNKNGI KTLCDKDGNP IQLRGMSTHG LQWFPGVVNN NAFAALSNDW
110 120 130 140 150
NSNVIRLAMY VAEGGYATNP SVKQTVINGI NYAIANDMYV IVDWHMMNPG
160 170 180 190 200
DPNASVYSGA QSFFNDISTL YPNNKNIIYE LCNEPNGENG GVTNDATGWA
210 220 230 240 250
QVKSYATPIV QLLRNKGNEN LIIVGNPFWS QRPDLAADNP INDSNTMYSV
260 270 280 290 300
HFYSGTNPIS TVDTNRDNAM SNVRYALNHG AAVFATEWGT SLATGTTGPY
310 320 330 340 350
LAKADAWLDF LNGNNISWCN FSISNKDEKA AALNSLTSLD PGSDKLWADN
360 370 380 390 400
ELTTSGQYVR ARIKGAYYAT PVDPVTNQPT APKDFSSGFW DFNDGTTQGF
410 420 430 440 450
GVNPDSPITA INVENANNAL KISNLNSKGS NDLSEGNFWA NVRISADIWG
460 470 480 490 500
QSINIYGDTK LTMDVIAPTP VNVSIAAIPQ SSTHGWGNPT RAIRVWTNNF
510 520 530 540 550
VAQTDGTYKA TLTISTNDSP NFNTIATDAA DSVVTNMILF VGSNSDNISL

DNIKFTK
Length:557
Mass (Da):60,131
Last modified:May 1, 1997 - v1
Checksum:iD186EC88EB504EED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37056 Genomic DNA. Translation: AAB40891.1.
PIRiJC5487.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37056 Genomic DNA. Translation: AAB40891.1.
PIRiJC5487.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J83X-ray1.70A/B378-557[»]
1J84X-ray2.02A378-557[»]
ProteinModelPortaliP94622.
SMRiP94622. Positions 38-374, 378-557.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi573061.Clocel_3662.

Protein family/group databases

CAZyiCBM17. Carbohydrate-Binding Module Family 17.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4107QWR. Bacteria.
COG2730. LUCA.

Miscellaneous databases

EvolutionaryTraceiP94622.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR005086. CBM_fam_17/28.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF03424. CBM_17_28. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. High N.N.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Characterization of engF, a gene for a non-cellulosomal Clostridium cellulovorans endoglucanase."
    Sheweita S.A., Ichi-ishi A., Park J.S., Liu C., Malburg L.M.Jr., Doi R.H.
    Gene 182:163-167(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "Recognition of cello-oligosaccharides by a family 17 carbohydrate-binding module: an X-ray crystallographic, thermodynamic and mutagenic study."
    Notenboom V., Boraston A.B., Chiu P., Freelove A.C., Kilburn D.G., Rose D.R.
    J. Mol. Biol. 314:797-806(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 378-557 IN COMPLEX WITH CALCIUM.

Entry informationi

Entry nameiP94622_CLOCL
AccessioniPrimary (citable) accession number: P94622
Entry historyi
Integrated into UniProtKB/TrEMBL: May 1, 1997
Last sequence update: May 1, 1997
Last modified: March 16, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.