Endo-1,4-beta glucanase EngF - Clostridium cellulovorans

Preferred order of sections

Names and origin

Protein names

Submitted name:
Endo-1,4-beta glucanase EngF EMBL AAB40891.1
EC=3.2.1.4 EMBL AAB40891.1

Gene names

Name:

engF EMBL AAB40891.1

Organism

Clostridium cellulovorans EMBL AAB40891.1

Taxonomic identifier

1493 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 1J84 PDB 1J83 Metal-binding PDB 1J84 PDB 1J83
Molecular function	Glycosidase EMBL AAB40891.1 Hydrolase
Technical term	3D-structure PDB 1J84 PDB 1J83
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

391

1

Calcium; via carbonyl oxygen PDB 1J84 PDB 1J83

Metal binding

393

1

Calcium PDB 1J84 PDB 1J83

Metal binding

418

1

Calcium; via carbonyl oxygen PDB 1J84 PDB 1J83

Metal binding

551

1

Calcium PDB 1J84 PDB 1J83

Sequences

Sequence

Length

Mass (Da)

Tools

P94622 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: D186EC88EB504EED
Show »

FASTA

557

60,131

        10         20         30         40         50         60 
MFNNVKKKIL SIVAAGAMLM ALVPNINVAA ETTYSNLTGN ANVKKPSVGG KLQLLNKNGI 

        70         80         90        100        110        120 
KTLCDKDGNP IQLRGMSTHG LQWFPGVVNN NAFAALSNDW NSNVIRLAMY VAEGGYATNP 

       130        140        150        160        170        180 
SVKQTVINGI NYAIANDMYV IVDWHMMNPG DPNASVYSGA QSFFNDISTL YPNNKNIIYE 

       190        200        210        220        230        240 
LCNEPNGENG GVTNDATGWA QVKSYATPIV QLLRNKGNEN LIIVGNPFWS QRPDLAADNP 

       250        260        270        280        290        300 
INDSNTMYSV HFYSGTNPIS TVDTNRDNAM SNVRYALNHG AAVFATEWGT SLATGTTGPY 

       310        320        330        340        350        360 
LAKADAWLDF LNGNNISWCN FSISNKDEKA AALNSLTSLD PGSDKLWADN ELTTSGQYVR 

       370        380        390        400        410        420 
ARIKGAYYAT PVDPVTNQPT APKDFSSGFW DFNDGTTQGF GVNPDSPITA INVENANNAL 

       430        440        450        460        470        480 
KISNLNSKGS NDLSEGNFWA NVRISADIWG QSINIYGDTK LTMDVIAPTP VNVSIAAIPQ 

       490        500        510        520        530        540 
SSTHGWGNPT RAIRVWTNNF VAQTDGTYKA TLTISTNDSP NFNTIATDAA DSVVTNMILF 

       550 
VGSNSDNISL DNIKFTK

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References

[1]	"Characterization of engF, a gene for a non-cellulosomal Clostridium cellulovorans endoglucanase." Sheweita S.A., Ichi-ishi A., Park J.S., Liu C., Malburg L.M. Jr, Doi R.H. Gene 182:163-167(1996) [PubMed: 8982083] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	Sheweita S., Park J.-S., Doi R.H. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Recognition of cello-oligosaccharides by a family 17 carbohydrate-binding module: an X-ray crystallographic, thermodynamic and mutagenic study." Notenboom V., Boraston A.B., Chiu P., Freelove A.C., Kilburn D.G., Rose D.R. J. Mol. Biol. 314:797-806(2001) [PubMed: 11733998] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 378-557 IN COMPLEX WITH CALCIUM.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U37056 Genomic DNA. Translation: AAB40891.1.

PIR

JC5487.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1J83	X-ray	1.70	A/B	378-557	[»]
1J84	X-ray	2.02	A	378-557	[»]

ProteinModelPortal

P94622.

SMR

P94622. Positions 38-374, 378-557.

ModBase

Search...

Protein family/group databases

CAZy

CBM17. Carbohydrate-Binding Module Family 17.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR005086. CBM_fam_17/28.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

Pfam

PF03424. CBM_17_28. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]

SUPFAM

SSF49785. Gal_bind_like. 1 hit.
SSF51445. Glyco_hydro_cat. 1 hit.

PROSITE

PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

P94622_CLOCL

Accession

Primary (citable) accession number: P94622

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 1997
Last sequence update:	May 1, 1997
Last modified:	May 31, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)