P94616 (SYGA_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	CBU_1913

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP-Rule MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glycine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Glycine--tRNA ligase alpha subunit HAMAP-Rule MF_00254		PRO_0000072835
Regions
	Compositional bias	301 – 305	5	Poly-Ser HAMAP-Rule MF_00254

Sequences

	Sequence		Length	Mass (Da)
	P94616 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: FF52579A9F0787F8	FASTA	319	36,397
	10 20 30 40 50 60 MKSSHPVNFQ QMILALQEYW ASQGCVLLQP FDMEVGAGTF HPATFLRAIG PEPWRAAYVQ 70 80 90 100 110 120 PSRRPTDGRY GDNPNRTQHY YQFQVVLKPS PDDIQDIYLG SLKALGIDPL THDIRFVEDN 130 140 150 160 170 180 WEAPTLGSWG VGWEVWQDGM EITQFTYFQQ IGGLECKPVT GEITYGLERL AMFLQGIDNM 190 200 210 220 230 240 FDLVWTEGPN GRVTYGQIFQ QNEVEMSAYN FEYANVEALF NFFDFYEKEA SQLIEVHLPL 250 260 270 280 290 300 AAYEMVLKAS HTFNLLDARQ AISVTERQRF ILRVRKLAQA VAEAYYSARE KLGFPMLEEI 310 SSSSSRVLPL AGNDRVKGC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Willems H., Jaeger C. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.
[2]	"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y10435 Genomic DNA. Translation: CAA71456.1. AE016828 Genomic DNA. Translation: AAO91404.1.
RefSeq	NP_820890.1. NC_002971.3.
3D structure databases
ProteinModelPortal	P94616.
SMR	P94616. Positions 9-285.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1913.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO91404; AAO91404; CBU_1913.
GeneID	1209826.
KEGG	cbu:CBU_1913.
PATRIC	17932561. VBICoxBur82552_1897.
Phylogenomic databases
eggNOG	COG0752.
HOGENOM	HOG000264291.
KO	K01878.
OMA	CVILQPY.
ProtClustDB	PRK09348.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1887-MONOMER.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha.
InterPro	IPR006194. Gly-tRNA-synth_heterodimer. IPR002310. Gly-tRNA_ligase_asu. [Graphical view]
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. glyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_COXBU

Accession

Primary (citable) accession number: P94616

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program