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Protein

tRNA modification GTPase MnmE

Gene

mnmE

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.UniRule annotation

Cofactori

K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei25FormyltetrahydrofolateUniRule annotation1
Binding sitei81FormyltetrahydrofolateUniRule annotation1
Binding sitei120FormyltetrahydrofolateUniRule annotation1
Metal bindingi226PotassiumUniRule annotation1
Metal bindingi230MagnesiumUniRule annotation1
Metal bindingi245Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi247Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi250PotassiumUniRule annotation1
Metal bindingi251MagnesiumUniRule annotation1
Binding sitei452FormyltetrahydrofolateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi226 – 231GTPUniRule annotation6
Nucleotide bindingi245 – 251GTPUniRule annotation7
Nucleotide bindingi270 – 273GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processtRNA processing
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA modification GTPase MnmEUniRule annotation (EC:3.6.-.-UniRule annotation)
Gene namesi
Name:mnmEUniRule annotation
Synonyms:trmEUniRule annotation
Ordered Locus Names:CBU_1922
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

P94612:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001888721 – 452tRNA modification GTPase MnmEAdd BLAST452

Proteomic databases

PRIDEiP94612.

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_1922.

Structurei

3D structure databases

ProteinModelPortaliP94612.
SMRiP94612.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini216 – 375TrmE-type GAdd BLAST160

Sequence similaritiesi

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1H. Bacteria.
COG0486. LUCA.
HOGENOMiHOG000200714.
KOiK03650.
OMAiCEIQCHG.

Family and domain databases

CDDicd04164. trmE. 1 hit.
Gene3Di1.20.120.430. 2 hits.
HAMAPiMF_00379. GTPase_MnmE. 1 hit.
InterProiView protein in InterPro
IPR031168. G_TrmE.
IPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR027368. MnmE_dom2.
IPR025867. MnmE_helical.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
PfamiView protein in Pfam
PF01926. MMR_HSR1. 1 hit.
PF12631. MnmE_helical. 1 hit.
PF10396. TrmE_N. 1 hit.
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF116878. SSF116878. 2 hits.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51709. G_TRME. 1 hit.

Sequencei

Sequence statusi: Complete.

P94612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYVFPETIA AQATPSGRGG IGVVRVSGEK TKAIAQKILG CVPKPRYATF
60 70 80 90 100
VKFRDSGSVI DEGIALYFPK PNSFTGEDVL ELHGHGGPVV MDRLLNTVLK
110 120 130 140 150
AGARQARPGE FSERAFLNNK IDLAQAEAVA DLINASSEQA ARSAMRSLQG
160 170 180 190 200
EFSKRIHQLV DALIQLRMYI EASIDFPEEE IDFLADERIK ETLENLTHQV
210 220 230 240 250
QEIEKTAKQG ALLREGITVV IAGEPNVGKS SLLNLLSGQE TAIVTDIAGT
260 270 280 290 300
TRDIIRESIH IDGLPIHVVD TAGLRLTEDV VEKEGVRRTQ KAVQQADLLL
310 320 330 340 350
LMIDASKPTE DFKKIIAQWF SENDNKIPTL IVENKIDLIG EAPRKENKEY
360 370 380 390 400
PHIKLSVKTR AGVELLKNHL KNTAGFEATH ENNFIARRRH CDAIARASAF
410 420 430 440 450
LKNANNHLLN QKAGELVAED LKLAQNALSE ITGEFTSDDL LGKIFSEFCI

GK
Length:452
Mass (Da):49,731
Last modified:May 1, 1997 - v1
Checksum:iACD16CD07DAC75EA
GO

Sequence cautioni

P94612: The sequence AAO91413 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10436 Genomic DNA. Translation: CAA71458.1.
AE016828 Genomic DNA. Translation: AAO91413.1. Different initiation.
RefSeqiNP_820899.1. NC_002971.3.

Genome annotation databases

EnsemblBacteriaiAAO91413; AAO91413; CBU_1922.
GeneIDi1209835.
KEGGicbu:CBU_1922.
PATRICifig|227377.7.peg.1905.

Similar proteinsi

Entry informationi

Entry nameiMNME_COXBU
AccessioniPrimary (citable) accession number: P94612
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 1997
Last modified: October 25, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families