P94605 (GYRA_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	CA_C0007

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	830 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 830	830	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145231
Sites
	Active site	121	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Sequence conflict	766 – 768	3	GIA → HS in AAB41131. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P94605 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: 1508C0CB16E82252	FASTA	830	92,612
	10 20 30 40 50 60 MLNEGKVLPV DISSEMKKCY IDYAMSVIVS RALPDVRDGL KPVHRRILYS MHELGLTPEK 70 80 90 100 110 120 GYRKCARIVG DVLGKYHPHG DSSVYGALVR LAQDFNLRYT VVDGHGNFGS VDGDSAAAMR 130 140 150 160 170 180 YTEAKMNKIA LEMVRDIGKN TVDFIPNFDG EEKEPVVLPS RFPNLLVNGS AGIAVGMATN 190 200 210 220 230 240 IPPHNLGEVI DGITMLIDNP EATILELMAQ IKGPDFPTAG IIMGKSGIRA AYETGRGKIT 250 260 270 280 290 300 VRAKSEIEVE DNGKQKIIIT EIPYQVNKAR LVESIADLVK DKRIVGISDL RDESDRDGMR 310 320 330 340 350 360 IVIEIKKDAN SNIILNQLYK HTRLQDTFGI NMLALVDNRP EVLNLKQILQ HYIKFQEQVI 370 380 390 400 410 420 RRRTEFDLEK ASARAHILEG LKIALDHIDE VISLIRGSKT AQEAKLGLMD KFGLSEKQAQ 430 440 450 460 470 480 AILDLKLQRL TGLEREKIED EYNELMKTIA ELKSILADEN KILAIIRDEL NEIKAKYGDE 490 500 510 520 530 540 RKTAIERAEN DIDIEDLIQE ENVVITLTHA GYIKRINADT YTSQKRGGRG IQAMTTKEDD 550 560 570 580 590 600 FVENIFITST HNNILFFTNK GRMYKLKAYQ IPEAGRAAKG TNVVNLLQLD PNEKIQAVIS 610 620 630 640 650 660 IKEFDEESFL VMCTKKGIIK KTVVGMYKNI RKSGLIAINL NDDDELVSVR ITKGDDDIII 670 680 690 700 710 720 VTNKGLAIRF NEVDVRPLGR NALGVKGITL KEDDFVVGME VPNQESDVLV VSENGFGKRT 730 740 750 760 770 780 HVGEYKCQHR GGKGLITYKV SDKTGKLVGV RMVEDGDELM LINNLGIAIR INVSDISTTS 790 800 810 820 830 RNAMGVTLMR NNGDEKVLAL AKINKDDSEQ LEDSEEVSEV HDAEENNSEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and molecular characterization of the DNA gyrase genes from Clostridium acetobutylicum." Ullmann S., Duerre P. Anaerobe 2:239-248(1996) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U35453 Genomic DNA. Translation: AAB41131.1. AE001437 Genomic DNA. Translation: AAK77994.1.
PIR	G96900. T46556.
RefSeq	NP_346654.1. NC_003030.1.
3D structure databases
ProteinModelPortal	P94605.
SMR	P94605. Positions 29-485.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1116190.
GenomeReviews	Gene locus CA_C0007 in contig AE001437_GR.
KEGG	cac:CA_C0007.
NMPDR	fig|272562.1.peg.183.
PATRIC	32034257. VBICloAce74127_0186.
Phylogenomic databases
HOGENOM	HBG635183.
OMA	TGRGRIY.
PhylomeDB	P94605.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	CACE272562:CAC0007-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_CLOAB

Accession

Primary (citable) accession number: P94605

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 29, 2001
Last modified:	January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program