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Protein

Phosphatase YwpJ

Gene

ywpJ

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dephosphorylation of phosphorylated 5-6 carbon sugars and monophosphate nucleotides (NMP) in vitro (By similarity). To a lesser extent, dephosphorylates flavin mononucleotide (FMN) in vitro (PubMed:26316208).By similarity1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

    1. Vmax=0.009 µmol/min/mg enzyme with flavin mononucleotide1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei7 – 71NucleophileBy similarity
    Metal bindingi7 – 71MagnesiumCurated
    Binding sitei8 – 81Phosphate; via amide nitrogenBy similarity
    Metal bindingi9 – 91Magnesium; via carbonyl oxygenCurated
    Binding sitei214 – 2141Phosphate1 Publication
    Metal bindingi237 – 2371MagnesiumCurated
    Metal bindingi238 – 2381MagnesiumCurated
    Binding sitei240 – 2401Phosphate1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU36290-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatase YwpJ1 Publication (EC:3.1.3.-1 Publication)
    Gene namesi
    Name:ywpJ
    Ordered Locus Names:BSU36290
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 285285Phosphatase YwpJPRO_0000360521Add
    BLAST

    Proteomic databases

    PaxDbiP94592.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100019621.

    Structurei

    Secondary structure

    1
    285
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 75Combined sources
    Helixi8 – 125Combined sources
    Helixi21 – 3212Combined sources
    Beta strandi36 – 405Combined sources
    Helixi45 – 528Combined sources
    Turni53 – 564Combined sources
    Beta strandi60 – 634Combined sources
    Helixi64 – 663Combined sources
    Beta strandi68 – 703Combined sources
    Beta strandi76 – 794Combined sources
    Helixi84 – 9613Combined sources
    Beta strandi100 – 1078Combined sources
    Beta strandi109 – 1113Combined sources
    Helixi115 – 12915Combined sources
    Helixi135 – 14713Combined sources
    Beta strandi151 – 1533Combined sources
    Helixi157 – 1604Combined sources
    Beta strandi162 – 1654Combined sources
    Beta strandi169 – 1757Combined sources
    Helixi179 – 18911Combined sources
    Beta strandi195 – 1984Combined sources
    Beta strandi204 – 2096Combined sources
    Helixi214 – 22411Combined sources
    Helixi229 – 2313Combined sources
    Beta strandi232 – 2387Combined sources
    Helixi239 – 2413Combined sources
    Helixi242 – 2476Combined sources
    Beta strandi248 – 2536Combined sources
    Helixi259 – 2646Combined sources
    Beta strandi266 – 2683Combined sources
    Helixi272 – 2743Combined sources
    Helixi276 – 2838Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NRWX-ray1.70A1-285[»]
    ProteinModelPortaliP94592.
    SMRiP94592. Positions 1-285.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP94592.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni41 – 422Phosphate binding
    Regioni282 – 2832Phosphate binding

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG410667J. Bacteria.
    COG0561. LUCA.
    HOGENOMiHOG000184780.
    InParanoidiP94592.
    KOiK07024.
    OMAiESENYYY.
    OrthoDBiEOG6K13W0.
    PhylomeDBiP94592.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR000150. Hypothet_cof.
    [Graphical view]
    PfamiPF08282. Hydrolase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR00099. Cof-subfamily. 1 hit.
    TIGR01484. HAD-SF-IIB. 1 hit.
    PROSITEiPS01228. COF_1. 1 hit.
    PS01229. COF_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P94592-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKLIAIDLDG TLLNSKHQVS LENENALRQA QRDGIEVVVS TGRAHFDVMS
    60 70 80 90 100
    IFEPLGIKTW VISANGAVIH DPEGRLYHHE TIDKKRAYDI LSWLESENYY
    110 120 130 140 150
    YEVFTGSAIY TPQNGRELLD VELDRFRSAN PEADLSVLKQ AAEVQYSQSG
    160 170 180 190 200
    FAYINSFQEL FEADEPIDFY NILGFSFFKE KLEAGWKRYE HAEDLTLVSS
    210 220 230 240 250
    AEHNFELSSR KASKGQALKR LAKQLNIPLE ETAAVGDSLN DKSMLEAAGK
    260 270 280
    GVAMGNARED IKSIADAVTL TNDEHGVAHM MKHLL
    Length:285
    Mass (Da):31,955
    Last modified:July 1, 1997 - v2
    Checksum:i003BFFF619CBC439
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z83337 Genomic DNA. Translation: CAB05952.1.
    AL009126 Genomic DNA. Translation: CAB15646.1.
    PIRiC70066.
    RefSeqiNP_391510.1. NC_000964.3.
    WP_003242946.1. NZ_JNCM01000034.1.

    Genome annotation databases

    EnsemblBacteriaiCAB15646; CAB15646; BSU36290.
    GeneIDi936907.
    KEGGibsu:BSU36290.
    PATRICi18979280. VBIBacSub10457_3802.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z83337 Genomic DNA. Translation: CAB05952.1.
    AL009126 Genomic DNA. Translation: CAB15646.1.
    PIRiC70066.
    RefSeqiNP_391510.1. NC_000964.3.
    WP_003242946.1. NZ_JNCM01000034.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NRWX-ray1.70A1-285[»]
    ProteinModelPortaliP94592.
    SMRiP94592. Positions 1-285.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100019621.

    Proteomic databases

    PaxDbiP94592.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB15646; CAB15646; BSU36290.
    GeneIDi936907.
    KEGGibsu:BSU36290.
    PATRICi18979280. VBIBacSub10457_3802.

    Phylogenomic databases

    eggNOGiENOG410667J. Bacteria.
    COG0561. LUCA.
    HOGENOMiHOG000184780.
    InParanoidiP94592.
    KOiK07024.
    OMAiESENYYY.
    OrthoDBiEOG6K13W0.
    PhylomeDBiP94592.

    Enzyme and pathway databases

    BioCyciBSUB:BSU36290-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiP94592.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR000150. Hypothet_cof.
    [Graphical view]
    PfamiPF08282. Hydrolase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR00099. Cof-subfamily. 1 hit.
    TIGR01484. HAD-SF-IIB. 1 hit.
    PROSITEiPS01228. COF_1. 1 hit.
    PS01229. COF_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
      Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
      Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Catalysis of an essential step in Vitamin B2 biosynthesis by a consortium of broad spectrum hydrolases."
      Sarge S., Haase I., Illarionov B., Laudert D., Hohmann H.P., Bacher A., Fischer M.
      ChemBioChem 16:2466-2469(2015) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR.
    4. "The structure of a haloacid dehalogenase-like hydrolase from B. subtilis."
      Midwest center for structural genomics (MCSG)
      Submitted (JAN-2005) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH PHOSPHATE AND CALCIUM IONS.

    Entry informationi

    Entry nameiYWPJ_BACSU
    AccessioniPrimary (citable) accession number: P94592
    Secondary accession number(s): Q795B2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: July 1, 1997
    Last modified: February 17, 2016
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.