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Protein

Phosphatase YwpJ

Gene

ywpJ

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dephosphorylation of phosphorylated 5-6 carbon sugars and monophosphate nucleotides (NMP) in vitro (By similarity). To a lesser extent, dephosphorylates flavin mononucleotide (FMN) in vitro (PubMed:26316208).By similarity1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

    1. Vmax=0.009 µmol/min/mg enzyme with flavin mononucleotide1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei7NucleophileBy similarity1
    Metal bindingi7MagnesiumCurated1
    Binding sitei8Phosphate; via amide nitrogenBy similarity1
    Metal bindingi9Magnesium; via carbonyl oxygenCurated1
    Binding sitei214Phosphate1 Publication1
    Metal bindingi237MagnesiumCurated1
    Metal bindingi238MagnesiumCurated1
    Binding sitei240Phosphate1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU36290-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatase YwpJ1 Publication (EC:3.1.3.-1 Publication)
    Gene namesi
    Name:ywpJ
    Ordered Locus Names:BSU36290
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003605211 – 285Phosphatase YwpJAdd BLAST285

    Proteomic databases

    PaxDbiP94592.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100019621.

    Structurei

    Secondary structure

    1285
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi3 – 7Combined sources5
    Helixi8 – 12Combined sources5
    Helixi21 – 32Combined sources12
    Beta strandi36 – 40Combined sources5
    Helixi45 – 52Combined sources8
    Turni53 – 56Combined sources4
    Beta strandi60 – 63Combined sources4
    Helixi64 – 66Combined sources3
    Beta strandi68 – 70Combined sources3
    Beta strandi76 – 79Combined sources4
    Helixi84 – 96Combined sources13
    Beta strandi100 – 107Combined sources8
    Beta strandi109 – 111Combined sources3
    Helixi115 – 129Combined sources15
    Helixi135 – 147Combined sources13
    Beta strandi151 – 153Combined sources3
    Helixi157 – 160Combined sources4
    Beta strandi162 – 165Combined sources4
    Beta strandi169 – 175Combined sources7
    Helixi179 – 189Combined sources11
    Beta strandi195 – 198Combined sources4
    Beta strandi204 – 209Combined sources6
    Helixi214 – 224Combined sources11
    Helixi229 – 231Combined sources3
    Beta strandi232 – 238Combined sources7
    Helixi239 – 241Combined sources3
    Helixi242 – 247Combined sources6
    Beta strandi248 – 253Combined sources6
    Helixi259 – 264Combined sources6
    Beta strandi266 – 268Combined sources3
    Helixi272 – 274Combined sources3
    Helixi276 – 283Combined sources8

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1NRWX-ray1.70A1-285[»]
    ProteinModelPortaliP94592.
    SMRiP94592.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP94592.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni41 – 42Phosphate binding2
    Regioni282 – 283Phosphate binding2

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG410667J. Bacteria.
    COG0561. LUCA.
    HOGENOMiHOG000184780.
    InParanoidiP94592.
    OMAiNTWVISA.
    PhylomeDBiP94592.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR000150. Hypothet_cof.
    [Graphical view]
    PfamiPF08282. Hydrolase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR00099. Cof-subfamily. 1 hit.
    TIGR01484. HAD-SF-IIB. 1 hit.
    PROSITEiPS01228. COF_1. 1 hit.
    PS01229. COF_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P94592-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKLIAIDLDG TLLNSKHQVS LENENALRQA QRDGIEVVVS TGRAHFDVMS
    60 70 80 90 100
    IFEPLGIKTW VISANGAVIH DPEGRLYHHE TIDKKRAYDI LSWLESENYY
    110 120 130 140 150
    YEVFTGSAIY TPQNGRELLD VELDRFRSAN PEADLSVLKQ AAEVQYSQSG
    160 170 180 190 200
    FAYINSFQEL FEADEPIDFY NILGFSFFKE KLEAGWKRYE HAEDLTLVSS
    210 220 230 240 250
    AEHNFELSSR KASKGQALKR LAKQLNIPLE ETAAVGDSLN DKSMLEAAGK
    260 270 280
    GVAMGNARED IKSIADAVTL TNDEHGVAHM MKHLL
    Length:285
    Mass (Da):31,955
    Last modified:July 1, 1997 - v2
    Checksum:i003BFFF619CBC439
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z83337 Genomic DNA. Translation: CAB05952.1.
    AL009126 Genomic DNA. Translation: CAB15646.1.
    PIRiC70066.
    RefSeqiNP_391510.1. NC_000964.3.
    WP_003242946.1. NZ_JNCM01000034.1.

    Genome annotation databases

    EnsemblBacteriaiCAB15646; CAB15646; BSU36290.
    GeneIDi936907.
    KEGGibsu:BSU36290.
    PATRICi18979280. VBIBacSub10457_3802.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z83337 Genomic DNA. Translation: CAB05952.1.
    AL009126 Genomic DNA. Translation: CAB15646.1.
    PIRiC70066.
    RefSeqiNP_391510.1. NC_000964.3.
    WP_003242946.1. NZ_JNCM01000034.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1NRWX-ray1.70A1-285[»]
    ProteinModelPortaliP94592.
    SMRiP94592.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100019621.

    Proteomic databases

    PaxDbiP94592.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB15646; CAB15646; BSU36290.
    GeneIDi936907.
    KEGGibsu:BSU36290.
    PATRICi18979280. VBIBacSub10457_3802.

    Phylogenomic databases

    eggNOGiENOG410667J. Bacteria.
    COG0561. LUCA.
    HOGENOMiHOG000184780.
    InParanoidiP94592.
    OMAiNTWVISA.
    PhylomeDBiP94592.

    Enzyme and pathway databases

    BioCyciBSUB:BSU36290-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiP94592.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR000150. Hypothet_cof.
    [Graphical view]
    PfamiPF08282. Hydrolase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR00099. Cof-subfamily. 1 hit.
    TIGR01484. HAD-SF-IIB. 1 hit.
    PROSITEiPS01228. COF_1. 1 hit.
    PS01229. COF_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiYWPJ_BACSU
    AccessioniPrimary (citable) accession number: P94592
    Secondary accession number(s): Q795B2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: July 1, 1997
    Last modified: November 2, 2016
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.