P94585 (MSCL_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Large-conductance mechanosensitive channel

Gene names

Name:	mscL
Synonyms:	ywpC
Ordered Locus Names:	BSU36360

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	130 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell By similarity. HAMAP MF_00115
Subunit structure	Homopentamer By similarity. HAMAP MF_00115
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_00115.
Sequence similarities	Belongs to the MscL family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ionic channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 130	130	Large-conductance mechanosensitive channel HAMAP MF_00115		PRO_0000192433
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 40	27	Helical; Potential
	Topological domain	41 – 65	25	Extracellular Potential
	Transmembrane	66 – 87	22	Helical; Potential
	Topological domain	88 – 130	43	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P94585 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 0941C01C2AAA8A9A	FASTA	130	14,280
	10 20 30 40 50 60 MWNEFKAFAM RGNIVDLAIG VVIGGAFGKI VTSLVNDIIM PLVGLLLGGL DFSGLSFTFG 70 80 90 100 110 120 DAVVKYGSFI QTIVNFLIIS FSIFIVIRTL NGLRRKKEAE EEAAEEAVDA QEELLKEIRD 130 LLKQQAKSPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)." Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P. Microbiology 143:3313-3328(1997) [PubMed: 9353933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z83337 Genomic DNA. Translation: CAB05944.1. AL009126 Genomic DNA. Translation: CAB15653.1.
PIR	E70065.
RefSeq	NP_391517.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P94585.
SMR	P94585. Positions 1-126.
ModBase	Search...
Protein family/group databases
TCDB	1.A.22.1.3. large conductance mechanosensitive ion channel (MscL) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000001553; EBBACP00000001553; EBBACG00000001551.
GeneID	936909.
GenomeReviews	Gene locus BSU36360 in contig AL009126_GR.
KEGG	bsu:BSU36360.
NMPDR	fig|224308.1.peg.3643.
PATRIC	18979298. VBIBacSub10457_3811.
Organism-specific databases
GenoList	BSU36360. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000002096.
HOGENOM	HBG727604.
OMA	VIDFVIV.
PhylomeDB	P94585.
ProtClustDB	PRK13955.
Enzyme and pathway databases
BioCyc	BSUB:BSU36360-MONOMER.
Family and domain databases
HAMAP	MF_00115. MscL. [Tree]
InterPro	IPR019823. Mechanosensitive_channel_CS. IPR001185. MS_channel. [Graphical view]
KO	K03282.
Pfam	PF01741. MscL. 1 hit. [Graphical view]
PRINTS	PR01264. MECHCHANNEL.
SUPFAM	SSF81330. MS_channel. 1 hit.
TIGRFAMs	TIGR00220. MscL. 1 hit.
PROSITE	PS01327. MSCL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSCL_BACSU

Accession

Primary (citable) accession number: P94585

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 1, 1997
Last modified:	January 25, 2012
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program