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P94571 (BCRC_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Undecaprenyl-diphosphatase BcrC

EC=3.6.1.27
Alternative name(s):
Undecaprenyl pyrophosphate phosphatase
Gene names
Name:bcrC
Synonyms:ywoA
Ordered Locus Names:BSU36530
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length193 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin. Ref.3 Ref.4 Ref.6

Catalytic activity

Ditrans,octacis-undecaprenyl diphosphate + H2O = ditrans,octacis-undecaprenyl phosphate + phosphate. Ref.6

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.6.

Induction

Expression is sigma M, sigma X and sigma I-dependent. Induced by vancomycin and bacitracin, via sigma M. Expression is maximal during early exponential phase and decreases during stationary phase. Ref.3 Ref.5 Ref.7

Disruption phenotype

Disruption results in a marked decrease of resistance to bacitracin. Ref.5

Miscellaneous

Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available.

Sequence similarities

Belongs to the BcrC/YbjG family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 193193Undecaprenyl-diphosphatase BcrC
PRO_0000049990

Regions

Transmembrane24 – 4421Helical; Potential
Transmembrane53 – 7321Helical; Potential
Transmembrane119 – 13921Helical; Potential
Transmembrane140 – 16021Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
P94571 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: E7C1ACC1556BC6CE

FASTA19321,723
        10         20         30         40         50         60 
MNYEIFKAIH GLSHHNSVLD SIMVFITEYA IVAYALILLA IWLFGNTQSR KHVLYAGITG 

        70         80         90        100        110        120 
IAGLVINYLI TLVYFEPRPF VAHTVHTLIP HAADASFPSD HTTGALAISI AMLFRNRKIG 

       130        140        150        160        170        180 
WPLVIFGLLT GFSRIWVGHH YPVDVLGSLV VAIIIGFLFF RFSDLLRPFV DLVVRIYEAI 

       190 
INKLTKKPTD QNF 

« Hide

References

« Hide 'large scale' references
[1]"The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Regulation of the Bacillus subtilis bcrC bacitracin resistance gene by two extracytoplasmic function sigma factors."
Cao M., Helmann J.D.
J. Bacteriol. 184:6123-6129(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN BACITRACIN RESISTANCE, INDUCTION.
Strain: 168 / CU1065.
[4]"YtsCD and YwoA, two independent systems that confer bacitracin resistance to Bacillus subtilis."
Bernard R., Joseph P., Guiseppi A., Chippaux M., Denizot F.
FEMS Microbiol. Lett. 228:93-97(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: 168.
[5]"A bacitracin-resistant Bacillus subtilis gene encodes a homologue of the membrane-spanning subunit of the Bacillus licheniformis ABC transporter."
Ohki R., Tateno K., Okada Y., Okajima H., Asai K., Sadaie Y., Murata M., Aiso T.
J. Bacteriol. 185:51-59(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, DISRUPTION PHENOTYPE.
Strain: 168.
[6]"BcrC from Bacillus subtilis acts as an undecaprenyl pyrophosphate phosphatase in bacitracin resistance."
Bernard R., El Ghachi M., Mengin-Lecreulx D., Chippaux M., Denizot F.
J. Biol. Chem. 280:28852-28857(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
Strain: 168.
[7]"Genetic evidence for the actin homolog gene mreBH and the bacitracin resistance gene bcrC as targets of the alternative sigma factor sigI of Bacillus subtilis."
Tseng C.-L., Shaw G.-C.
J. Bacteriol. 190:1561-1567(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z82987 Genomic DNA. Translation: CAB05382.1.
AL009126 Genomic DNA. Translation: CAB15670.1.
PIRD70064.
RefSeqNP_391534.1. NC_000964.3.

3D structure databases

ProteinModelPortalP94571.
SMRP94571. Positions 64-161.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU36530.

Protein family/group databases

TCDB9.B.105.1.3. the lead resistance fusion protein (pbrbc) family.

Proteomic databases

PaxDbP94571.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15670; CAB15670; BSU36530.
GeneID936946.
KEGGbsu:BSU36530.
PATRIC18979330. VBIBacSub10457_3827.

Organism-specific databases

GenoListBSU36530. [Micado]

Phylogenomic databases

eggNOGCOG0671.
HOGENOMHOG000221066.
OMARIWVGHH.
OrthoDBEOG661HBQ.
PhylomeDBP94571.

Enzyme and pathway databases

BioCycBSUB:BSU36530-MONOMER.

Family and domain databases

Gene3D1.20.144.10. 1 hit.
InterProIPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamPF01569. PAP2. 1 hit.
[Graphical view]
SMARTSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMSSF48317. SSF48317. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBCRC_BACSU
AccessionPrimary (citable) accession number: P94571
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList