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Protein

Undecaprenyl-diphosphatase BcrC

Gene

bcrC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.3 Publications

Catalytic activityi

Ditrans,octacis-undecaprenyl diphosphate + H2O = ditrans,octacis-undecaprenyl phosphate + phosphate.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciBSUB:BSU36530-MONOMER.
BRENDAi3.6.1.27. 658.

Protein family/group databases

TCDBi9.B.105.1.3. the lead resistance fusion protein (pbrbc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Undecaprenyl-diphosphatase BcrC (EC:3.6.1.27)
Alternative name(s):
Undecaprenyl pyrophosphate phosphatase
Gene namesi
Name:bcrC
Synonyms:ywoA
Ordered Locus Names:BSU36530
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU36530. [Micado]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei24 – 4421HelicalSequence AnalysisAdd
BLAST
Transmembranei53 – 7321HelicalSequence AnalysisAdd
BLAST
Transmembranei119 – 13921HelicalSequence AnalysisAdd
BLAST
Transmembranei140 – 16021HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption results in a marked decrease of resistance to bacitracin.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 193193Undecaprenyl-diphosphatase BcrCPRO_0000049990Add
BLAST

Proteomic databases

PaxDbiP94571.

Expressioni

Inductioni

Expression is sigma M, sigma X and sigma I-dependent. Induced by vancomycin and bacitracin, via sigma M. Expression is maximal during early exponential phase and decreases during stationary phase.3 Publications

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019746.

Structurei

3D structure databases

ProteinModelPortaliP94571.
SMRiP94571. Positions 64-161.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BcrC/YbjG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0671.
HOGENOMiHOG000221066.
InParanoidiP94571.
OMAiRIWVGHH.
OrthoDBiEOG661HBQ.
PhylomeDBiP94571.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequencei

Sequence statusi: Complete.

P94571-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYEIFKAIH GLSHHNSVLD SIMVFITEYA IVAYALILLA IWLFGNTQSR
60 70 80 90 100
KHVLYAGITG IAGLVINYLI TLVYFEPRPF VAHTVHTLIP HAADASFPSD
110 120 130 140 150
HTTGALAISI AMLFRNRKIG WPLVIFGLLT GFSRIWVGHH YPVDVLGSLV
160 170 180 190
VAIIIGFLFF RFSDLLRPFV DLVVRIYEAI INKLTKKPTD QNF
Length:193
Mass (Da):21,723
Last modified:May 1, 1997 - v1
Checksum:iE7C1ACC1556BC6CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z82987 Genomic DNA. Translation: CAB05382.1.
AL009126 Genomic DNA. Translation: CAB15670.1.
PIRiD70064.
RefSeqiNP_391534.1. NC_000964.3.
WP_003243362.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB15670; CAB15670; BSU36530.
GeneIDi936946.
KEGGibsu:BSU36530.
PATRICi18979330. VBIBacSub10457_3827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z82987 Genomic DNA. Translation: CAB05382.1.
AL009126 Genomic DNA. Translation: CAB15670.1.
PIRiD70064.
RefSeqiNP_391534.1. NC_000964.3.
WP_003243362.1. NZ_JNCM01000034.1.

3D structure databases

ProteinModelPortaliP94571.
SMRiP94571. Positions 64-161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100019746.

Protein family/group databases

TCDBi9.B.105.1.3. the lead resistance fusion protein (pbrbc) family.

Proteomic databases

PaxDbiP94571.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15670; CAB15670; BSU36530.
GeneIDi936946.
KEGGibsu:BSU36530.
PATRICi18979330. VBIBacSub10457_3827.

Organism-specific databases

GenoListiBSU36530. [Micado]

Phylogenomic databases

eggNOGiCOG0671.
HOGENOMiHOG000221066.
InParanoidiP94571.
OMAiRIWVGHH.
OrthoDBiEOG661HBQ.
PhylomeDBiP94571.

Enzyme and pathway databases

BioCyciBSUB:BSU36530-MONOMER.
BRENDAi3.6.1.27. 658.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
    Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
    Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Regulation of the Bacillus subtilis bcrC bacitracin resistance gene by two extracytoplasmic function sigma factors."
    Cao M., Helmann J.D.
    J. Bacteriol. 184:6123-6129(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN BACITRACIN RESISTANCE, INDUCTION.
    Strain: 168 / CU1065.
  4. "YtsCD and YwoA, two independent systems that confer bacitracin resistance to Bacillus subtilis."
    Bernard R., Joseph P., Guiseppi A., Chippaux M., Denizot F.
    FEMS Microbiol. Lett. 228:93-97(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: 168.
  5. "A bacitracin-resistant Bacillus subtilis gene encodes a homologue of the membrane-spanning subunit of the Bacillus licheniformis ABC transporter."
    Ohki R., Tateno K., Okada Y., Okajima H., Asai K., Sadaie Y., Murata M., Aiso T.
    J. Bacteriol. 185:51-59(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, DISRUPTION PHENOTYPE.
    Strain: 168.
  6. "BcrC from Bacillus subtilis acts as an undecaprenyl pyrophosphate phosphatase in bacitracin resistance."
    Bernard R., El Ghachi M., Mengin-Lecreulx D., Chippaux M., Denizot F.
    J. Biol. Chem. 280:28852-28857(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
    Strain: 168.
  7. "Genetic evidence for the actin homolog gene mreBH and the bacitracin resistance gene bcrC as targets of the alternative sigma factor sigI of Bacillus subtilis."
    Tseng C.-L., Shaw G.-C.
    J. Bacteriol. 190:1561-1567(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: 168.

Entry informationi

Entry nameiBCRC_BACSU
AccessioniPrimary (citable) accession number: P94571
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: June 24, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.