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Protein

dITP/XTP pyrophosphatase

Gene

ysnA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

XTP + H2O = XMP + diphosphate.UniRule annotation
dITP + H2O = dIMP + diphosphate.UniRule annotation
ITP + H2O = IMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44MagnesiumUniRule annotation1
Active sitei73Proton acceptorUniRule annotation1
Metal bindingi73MagnesiumUniRule annotation1
Binding sitei74Substrate; via amide nitrogenUniRule annotation1
Binding sitei179SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU28360-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Name:ysnA
Ordered Locus Names:BSU28360
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001781291 – 198dITP/XTP pyrophosphataseAdd BLAST198

Proteomic databases

PaxDbiP94558.
PRIDEiP94558.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015491.

Structurei

3D structure databases

ProteinModelPortaliP94558.
SMRiP94558.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 16Substrate bindingUniRule annotation6
Regioni156 – 159Substrate bindingUniRule annotation4
Regioni184 – 185Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108V82. Bacteria.
COG0127. LUCA.
HOGENOMiHOG000293319.
InParanoidiP94558.
KOiK02428.
OMAiYDPIFQP.
PhylomeDBiP94558.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR020922. dITP/XTP_pyrophosphatase.
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

Sequencei

Sequence statusi: Complete.

P94558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIMKEAIIA THNPGKVKEF KEILEPRGYD VKSLAEIGFT EEIEETGHTF
60 70 80 90 100
EENAIMKAEA VAKAVNKMVI ADDSGLSIDN LGGRPGVYSA RYAGEQKDDQ
110 120 130 140 150
ANIEKVLSEL KGIEKEQRTA RFRCALAVSI PGEETKTVEG HVEGYIAEEP
160 170 180 190
RGEYGFGYDP IFIVKDKDKT MAELTSDEKN KISHRADALK KLSKLLEA
Length:198
Mass (Da):21,933
Last modified:May 1, 1997 - v1
Checksum:i217D1BDBFDBF2C19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75208 Genomic DNA. Translation: CAA99555.1.
AL009126 Genomic DNA. Translation: CAB14796.1.
PIRiC69986.
RefSeqiNP_390714.1. NC_000964.3.
WP_010886588.1. NZ_CP019663.1.

Genome annotation databases

EnsemblBacteriaiCAB14796; CAB14796; BSU28360.
GeneIDi937465.
KEGGibsu:BSU28360.
PATRICifig|224308.43.peg.2966.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75208 Genomic DNA. Translation: CAA99555.1.
AL009126 Genomic DNA. Translation: CAB14796.1.
PIRiC69986.
RefSeqiNP_390714.1. NC_000964.3.
WP_010886588.1. NZ_CP019663.1.

3D structure databases

ProteinModelPortaliP94558.
SMRiP94558.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015491.

Proteomic databases

PaxDbiP94558.
PRIDEiP94558.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14796; CAB14796; BSU28360.
GeneIDi937465.
KEGGibsu:BSU28360.
PATRICifig|224308.43.peg.2966.

Phylogenomic databases

eggNOGiENOG4108V82. Bacteria.
COG0127. LUCA.
HOGENOMiHOG000293319.
InParanoidiP94558.
KOiK02428.
OMAiYDPIFQP.
PhylomeDBiP94558.

Enzyme and pathway databases

BioCyciBSUB:BSU28360-MONOMER.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR020922. dITP/XTP_pyrophosphatase.
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIXTPA_BACSU
AccessioniPrimary (citable) accession number: P94558
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: May 1, 1997
Last modified: June 7, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.