P94549 (FADB_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable enoyl-CoA hydratase
EC=4.2.1.17

Gene names

Name:	fadB
Synonyms:	ysiB
Ordered Locus Names:	BSU28540

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the degradation of long-chain fatty acids.
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
Pathway	Lipid metabolism; fatty acid beta-oxidation.
Induction	Repressed by fadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize FadR as to its binding to fadR boxes on target DNA and thus derepress transcription. Ref.3
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid degradation Lipid metabolism
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Probable enoyl-CoA hydratase		PRO_0000360671

Sequences

	Sequence		Length	Mass (Da)
	P94549 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 5C1B3C3119C6F860	FASTA	258	27,554
	10 20 30 40 50 60 MNAISLAVDQ FVAVLTIHNP PANALSSRIL EELSSCLDQC ETDAGVRSII IHGEGRFFSA 70 80 90 100 110 120 GADIKEFTSL KGNEDSSLLA ERGQQLMERI ESFPKPIIAA IHGAALGGGL ELAMACHIRI 130 140 150 160 170 180 AAEDAKLGLP ELNLGIIPGF AGTQRLPRYV GTAKALELIG SGEPISGKEA LDLGLVSIGA 190 200 210 220 230 240 KDEAEVIEKA KALAAKFAEK SPQTLASLLE LLYSNKVYSY EGSLKLEAKR FGEAFESEDA 250 KEGIQAFLEK RKPQFKGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism." Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R. Microbiology 142:3067-3078(1996) [PubMed: 8969504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation." Matsuoka H., Hirooka K., Fujita Y. J. Biol. Chem. 282:5180-5194(2007) [PubMed: 17189250] [Abstract] Cited for: GENE NAME, INDUCTION. Strain: 168.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z75208 Genomic DNA. Translation: CAA99573.1. AL009126 Genomic DNA. Translation: CAB14814.1.
PIR	G69985.
RefSeq	NP_390732.1. NC_000964.3.
3D structure databases
HSSP	HSSP built from PDB template 1MJ3 based on UniProtKB P14604.
ProteinModelPortal	P94549.
SMR	P94549. Positions 1-257.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000000471; EBBACP00000000471; EBBACG00000000469.
GeneID	937448.
GenomeReviews	Gene locus BSU28540 in contig AL009126_GR.
KEGG	bsu:BSU28540.
NMPDR	fig|224308.1.peg.2857.
PATRIC	18977596. VBIBacSub10457_2986.
Organism-specific databases
GenoList	BSU28540. [Micado]
Phylogenomic databases
GeneTree	EBGT00070000031862.
HOGENOM	HBG748731.
OMA	ACHIRIA.
PhylomeDB	P94549.
ProtClustDB	PRK07658.
Enzyme and pathway databases
BioCyc	BSUB:BSU28540-MONOMER.
Family and domain databases
InterPro	IPR001753. Crotonase_core. IPR018376. Enoyl-CoA_hyd/isom_CS. [Graphical view]
KO	K13767.
Pfam	PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FADB_BACSU

Accession

Primary (citable) accession number: P94549
Secondary accession number(s): Q795X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	May 1, 1997
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program