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Protein

Long-chain-fatty-acid--CoA ligase

Gene

lcfA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. long-chain fatty acid-CoA ligase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU28560-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase (EC:6.2.1.3)
Alternative name(s):
Long-chain acyl-CoA synthetase
Gene namesi
Name:lcfA
Ordered Locus Names:BSU28560
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU28560. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560Long-chain-fatty-acid--CoA ligasePRO_0000193124Add
BLAST

Proteomic databases

PaxDbiP94547.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU28560.

Structurei

3D structure databases

ProteinModelPortaliP94547.
SMRiP94547. Positions 18-556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0318.
HOGENOMiHOG000229983.
InParanoidiP94547.
KOiK01897.
OMAiITFSDFH.
OrthoDBiEOG6MH5BV.
PhylomeDBiP94547.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P94547-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQSQKPWLAE YPNDIPHELP LPNKTLQSIL TDSAARFPDK TAISFYGKKL
60 70 80 90 100
TFHDILTDAL KLAAFLQCNG LQKGDRVAVM LPNCPQTVIS YYGVLFAGGI
110 120 130 140 150
VVQTNPLYTE HELEYQLRDA QVSVIITLDL LFPKAIKMKT LSIVDQILIT
160 170 180 190 200
SVKDYLPFPK NILYPLTQKQ KVHIDFDKTA NIHTFASCMK QEKTELLTIP
210 220 230 240 250
KIDPEHDIAV LQYTGGTTGA PKGVMLTHQN ILANTEMCAA WMYDVKEGAE
260 270 280 290 300
KVLGIVPFFH VYGLTAVMNY SIKLGFEMIL LPKFDPLETL KIIDKHKPTL
310 320 330 340 350
FPGAPTIYIG LLHHPELQHY DLSSIKSCLS GSAALPVEVK QKFEKVTGGK
360 370 380 390 400
LVEGYGLSEA SPVTHANFIW GKNKPGSIGC PWPSTDAAIY SEETGELAAP
410 420 430 440 450
YEHGEIIVKG PQVMKGYWNK PEETAAVLRD GWLFTGDMGY MDEEGFFYIA
460 470 480 490 500
DRKKDIIIAG GYNIYPREVE EALYEHEAIQ EIVVAGVPDS YRGETVKAFV
510 520 530 540 550
VLKKGAKADT EELDAFARSR LAPYKVPKAY EFRKELPKTA VGKILRRRLL
560
EEETENHHIK
Length:560
Mass (Da):62,692
Last modified:May 1, 1997 - v1
Checksum:i416A664E5926C7EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75208 Genomic DNA. Translation: CAA99571.1.
AL009126 Genomic DNA. Translation: CAB14816.1.
PIRiD69649.
RefSeqiNP_390734.1. NC_000964.3.
WP_004399166.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14816; CAB14816; BSU28560.
GeneIDi936505.
KEGGibsu:BSU28560.
PATRICi18977600. VBIBacSub10457_2988.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75208 Genomic DNA. Translation: CAA99571.1.
AL009126 Genomic DNA. Translation: CAB14816.1.
PIRiD69649.
RefSeqiNP_390734.1. NC_000964.3.
WP_004399166.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP94547.
SMRiP94547. Positions 18-556.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU28560.

Proteomic databases

PaxDbiP94547.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14816; CAB14816; BSU28560.
GeneIDi936505.
KEGGibsu:BSU28560.
PATRICi18977600. VBIBacSub10457_2988.

Organism-specific databases

GenoListiBSU28560. [Micado]

Phylogenomic databases

eggNOGiCOG0318.
HOGENOMiHOG000229983.
InParanoidiP94547.
KOiK01897.
OMAiITFSDFH.
OrthoDBiEOG6MH5BV.
PhylomeDBiP94547.

Enzyme and pathway databases

BioCyciBSUB:BSU28560-MONOMER.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism."
    Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R.
    Microbiology 142:3067-3078(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiLCFA_BACSU
AccessioniPrimary (citable) accession number: P94547
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: January 7, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.