Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P94547 (LCFA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Long-chain-fatty-acid--CoA ligase

EC=6.2.1.3
Alternative name(s):
Long-chain acyl-CoA synthetase
Gene names
Name:lcfA
Ordered Locus Names:BSU28560
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
   Biological processFatty acid metabolism
Lipid metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

long-chain fatty acid-CoA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 560560Long-chain-fatty-acid--CoA ligase
PRO_0000193124

Sequences

Sequence LengthMass (Da)Tools
P94547 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 416A664E5926C7EA

FASTA56062,692
        10         20         30         40         50         60 
MQSQKPWLAE YPNDIPHELP LPNKTLQSIL TDSAARFPDK TAISFYGKKL TFHDILTDAL 

        70         80         90        100        110        120 
KLAAFLQCNG LQKGDRVAVM LPNCPQTVIS YYGVLFAGGI VVQTNPLYTE HELEYQLRDA 

       130        140        150        160        170        180 
QVSVIITLDL LFPKAIKMKT LSIVDQILIT SVKDYLPFPK NILYPLTQKQ KVHIDFDKTA 

       190        200        210        220        230        240 
NIHTFASCMK QEKTELLTIP KIDPEHDIAV LQYTGGTTGA PKGVMLTHQN ILANTEMCAA 

       250        260        270        280        290        300 
WMYDVKEGAE KVLGIVPFFH VYGLTAVMNY SIKLGFEMIL LPKFDPLETL KIIDKHKPTL 

       310        320        330        340        350        360 
FPGAPTIYIG LLHHPELQHY DLSSIKSCLS GSAALPVEVK QKFEKVTGGK LVEGYGLSEA 

       370        380        390        400        410        420 
SPVTHANFIW GKNKPGSIGC PWPSTDAAIY SEETGELAAP YEHGEIIVKG PQVMKGYWNK 

       430        440        450        460        470        480 
PEETAAVLRD GWLFTGDMGY MDEEGFFYIA DRKKDIIIAG GYNIYPREVE EALYEHEAIQ 

       490        500        510        520        530        540 
EIVVAGVPDS YRGETVKAFV VLKKGAKADT EELDAFARSR LAPYKVPKAY EFRKELPKTA 

       550        560 
VGKILRRRLL EEETENHHIK 

« Hide

References

« Hide 'large scale' references
[1]"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism."
Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R.
Microbiology 142:3067-3078(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z75208 Genomic DNA. Translation: CAA99571.1.
AL009126 Genomic DNA. Translation: CAB14816.1.
PIRD69649.
RefSeqNP_390734.1. NC_000964.3.

3D structure databases

ProteinModelPortalP94547.
SMRP94547. Positions 18-556.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU28560.

Proteomic databases

PaxDbP94547.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14816; CAB14816; BSU28560.
GeneID936505.
KEGGbsu:BSU28560.
PATRIC18977600. VBIBacSub10457_2988.

Organism-specific databases

GenoListBSU28560. [Micado]

Phylogenomic databases

eggNOGCOG0318.
HOGENOMHOG000229983.
KOK01897.
OMATFSDFHD.
OrthoDBEOG6MH5BV.
ProtClustDBCLSK887693.

Enzyme and pathway databases

BioCycBSUB:BSU28560-MONOMER.

Family and domain databases

InterProIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLCFA_BACSU
AccessionPrimary (citable) accession number: P94547
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList