P94531 (ABFA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-N-arabinofuranosidase 1
Short name=Arabinosidase 1
EC=3.2.1.55

Gene names

Name:	abfA
Ordered Locus Names:	BSU28720

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.
Induction	Transcription is repressed by glucose and by the binding of AraR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of AraR to the DNA, thus allowing expression of the gene. Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 51 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-arabinose metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-N-arabinofuranosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Alpha-N-arabinofuranosidase 1		PRO_0000057701
Experimental info
	Sequence conflict	114	1	A → P in CAA99595. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P94531 [UniParc]. Last modified July 7, 2009. Version 2. Checksum: 3EEA8F8ADA3D705A	FASTA	500	57,034
	10 20 30 40 50 60 MKKARMIVDK EYKIGEVDKR IYGSFIEHMG RAVYEGIYEP DHPEADEDGF RKDVQSLIKE 70 80 90 100 110 120 LQVPIIRYPG GNFLSGYNWE DGVGPVENRP RRLDLAWQTT ETNEVGTNEF LSWAKKVNTE 130 140 150 160 170 180 VNMAVNLGTR GIDAARNLVE YCNHPKGSYW SDLRRSHGYE QPYGIKTWCL GNEMDGPWQI 190 200 210 220 230 240 GHKTADEYGR LAAETAKVMK WVDPSIELVA CGSSNSGMPT FIDWEAKVLE HTYEHVDYIS 250 260 270 280 290 300 LHTYYGNRDN NLPNYLARSM DLDHFIKSVA ATCDYVKAKT RSKKTINLSL DEWNVWYHSN 310 320 330 340 350 360 EADKKVEPWI TARPILEDIY NFEDALLVGS LLITMLQHAD RVKIACLAQL VNVIAPIMTE 370 380 390 400 410 420 KGGEAWRQPI FYPYMHASVY GRGESLKPLI SSPKYDCSDF TDVPYVDAAV VYSEEEETLT 430 440 450 460 470 480 IFAVNKAEDQ METEISLRGF ESYQIAEHIV LEHQDIKATN QHNRKNVVPH SNGSSSVSEN 490 500 GLTAHFTPLS WNVIRLKKQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression." Sa-Nogueira I.M.G., Nogueira T.V., Soares S., de Lencastre H. Microbiology 143:957-969(1997) [PubMed: 9084180] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism." Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R. Microbiology 142:3067-3078(1996) [PubMed: 8969504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 114.
[5]	"Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis." Mota L.J., Tavares P., Sa-Nogueira I.M.G. Mol. Microbiol. 33:476-489(1999) [PubMed: 10417639] [Abstract] Cited for: TRANSCRIPTIONAL REGULATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89810 Genomic DNA. Translation: CAA61937.1. Z75208 Genomic DNA. Translation: CAA99595.1. AL009126 Genomic DNA. Translation: CAB14832.2.
PIR	C69580.
RefSeq	NP_390750.2. NC_000964.3.
3D structure databases
ProteinModelPortal	P94531.
SMR	P94531. Positions 3-498.
ModBase	Search...
Protein family/group databases
CAZy	GH51. Glycoside Hydrolase Family 51.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000002808; EBBACP00000002808; EBBACG00000002803.
GeneID	937509.
GenomeReviews	Gene locus BSU28720 in contig AL009126_GR.
KEGG	bsu:BSU28720.
NMPDR	fig|224308.1.peg.2875.
PATRIC	18977636. VBIBacSub10457_3006.
Organism-specific databases
GenoList	BSU28720. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000002580.
HOGENOM	HBG654651.
PhylomeDB	P94531.
ProtClustDB	CLSK2539632.
Enzyme and pathway databases
BioCyc	BSUB:BSU28720-MONOMER. MetaCyc:BSU28720-MONOMER.
Family and domain databases
InterPro	IPR010720. Alpha-L-AF_C. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K01209.
Pfam	PF06964. Alpha-L-AF_C. 1 hit. [Graphical view]
SMART	SM00813. Alpha-L-AF_C. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ABFA_BACSU

Accession

Primary (citable) accession number: P94531
Secondary accession number(s): O05096

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 7, 2009
Last modified:	January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program