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Reviewed, UniProtKB/Swiss-Prot P94531 (ABFA_BACSU)

Last modified December 15, 2009. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Alpha-N-arabinofuranosidase 1
      Short name=Arabinosidase 1
    EC=3.2.1.55
Gene names
Name: abfA
Ordered Locus Names: BSU28720
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesBacillaceaeBacillus

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

Induction

Transcription is repressed by glucose and by the binding of araR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of araR to the DNA, thus allowing expression of the gene. Ref.5

Sequence similarities

Belongs to the glycosyl hydrolase 51 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processL-arabinose metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionalpha-N-arabinofuranosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500Alpha-N-arabinofuranosidase 1
PRO_0000057701

Experimental info

Sequence conflict1141A → P in CAA99595. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P94531-1 [UniParc].

Last modified July 7, 2009. Version 2.
Checksum: 3EEA8F8ADA3D705A

FASTA50057,034
        10         20         30         40         50         60 
MKKARMIVDK EYKIGEVDKR IYGSFIEHMG RAVYEGIYEP DHPEADEDGF RKDVQSLIKE 

        70         80         90        100        110        120 
LQVPIIRYPG GNFLSGYNWE DGVGPVENRP RRLDLAWQTT ETNEVGTNEF LSWAKKVNTE 

       130        140        150        160        170        180 
VNMAVNLGTR GIDAARNLVE YCNHPKGSYW SDLRRSHGYE QPYGIKTWCL GNEMDGPWQI 

       190        200        210        220        230        240 
GHKTADEYGR LAAETAKVMK WVDPSIELVA CGSSNSGMPT FIDWEAKVLE HTYEHVDYIS 

       250        260        270        280        290        300 
LHTYYGNRDN NLPNYLARSM DLDHFIKSVA ATCDYVKAKT RSKKTINLSL DEWNVWYHSN 

       310        320        330        340        350        360 
EADKKVEPWI TARPILEDIY NFEDALLVGS LLITMLQHAD RVKIACLAQL VNVIAPIMTE 

       370        380        390        400        410        420 
KGGEAWRQPI FYPYMHASVY GRGESLKPLI SSPKYDCSDF TDVPYVDAAV VYSEEEETLT 

       430        440        450        460        470        480 
IFAVNKAEDQ METEISLRGF ESYQIAEHIV LEHQDIKATN QHNRKNVVPH SNGSSSVSEN 

       490        500 
GLTAHFTPLS WNVIRLKKQS 

« Hide

References

« Hide 'large scale' references
[1]"The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression."
Sa-Nogueira I.M.G., Nogueira T.V., Soares S., de Lencastre H.
Microbiology 143:957-969(1997) [PubMed: 9084180] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism."
Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R.
Microbiology 142:3067-3078(1996) [PubMed: 8969504] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 114.
[5]"Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis."
Mota L.J., Tavares P., Sa-Nogueira I.M.G.
Mol. Microbiol. 33:476-489(1999) [PubMed: 10417639] [Abstract]
Cited for: TRANSCRIPTIONAL REGULATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X89810 Genomic DNA. Translation: CAA61937.1.
Z75208 Genomic DNA. Translation: CAA99595.1.
AL009126 Genomic DNA. Translation: CAB14832.2.
PIRC69580.
RefSeqNP_390750.2.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGH51. Glycoside Hydrolase Family 51.

Genome annotation databases

GeneID937509.
GenomeReviewsGene locus BSU28720 in contig AL009126_GR.
KEGGbsu:BSU28720.
NMPDRfig|224308.1.peg.2875.

Organism-specific databases

SubtiListBG11900. abfA. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMHBG654651.

Enzyme and pathway databases

BRENDA3.2.1.55. 150.

Family and domain databases

InterProIPR010720. Alpha-L-AF_C.
IPR017853. Glyco_hydro_catalytic_core.
[Graphical view]
PfamPF06964. Alpha-L-AF_C. 1 hit.
[Graphical view]
SMARTSM00813. Alpha-L-AF_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameABFA_BACSU
AccessionPrimary (citable) accession number: P94531
Secondary accession number(s): O05096
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 7, 2009
Last modified: December 15, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents