Reviewed, UniProtKB/Swiss-Prot P94524 (ARAB_BACSU)

Names and origin

Protein names

Recommended name:
    Ribulokinase
    EC=2.7.1.16

Gene names

Name:	araB
Ordered Locus Names:	BSU28790

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L(or D)-ribulose = ADP + L(or D)-ribulose 5-phosphate. HAMAP MF_00520
Pathway	Carbohydrate degradation; L-arabinose degradation via L-ribulose; D-xylulose 5-phosphate from L-arabinose (bacterial route): step 2/3. HAMAP MF_00520
Induction	Transcription is repressed by glucose and by the binding of araR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of araR to the DNA, thus allowing expression of the gene. Ref.4
Sequence similarities	Belongs to the ribulokinase family.

Ontologies

Keywords
Biological process	Arabinose catabolism Carbohydrate metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-arabinose catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ribulokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Ribulokinase HAMAP MF_00520		PRO_0000198357
Experimental info
	Sequence conflict	59	1	P → F in CAA61586. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P94524-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: DE72A1AB73AF0AEF	FASTA	560	61,010
	10 20 30 40 50 60 MAYTIGVDFG TLSGRAVLVH VQTGEELAAA VKEYRHAVID TVLPKTGQKL PRDWALQHPA 70 80 90 100 110 120 DYLEVLETTI PSLLEQTGVD PKDIIGIGID FTACTILPID SSGQPLCMLP EYEEEPHSYV 130 140 150 160 170 180 KLWKHHAAQK HADRLNQIAE EEGEAFLQRY GGKISSEWMI PKVMQIAEEA PHIYEAADRI 190 200 210 220 230 240 IEAADWIVYQ LCGSLKRSNC TAGYKAMWSE KAGYPSDDFF EKLNPSMKTI TKDKLSGSIH 250 260 270 280 290 300 SVGEKAGSLT EKMAKLTGLL PGTAVAVANV DAHVSVPAVG ITEPGKMLMI MGTSTCHVLL 310 320 330 340 350 360 GEEVHIVPGM CGVVDNGILP GYAGYEAGQS CVGDHFDWFV KTCVPPAYQE EAKEKNIGVH 370 380 390 400 410 420 ELLSEKANHQ APGESGLLAL DWWNGNRSTL VDADLTGMLL GMTLLTKPEE IYRALVEATA 430 440 450 460 470 480 YGTRMIIETF KESGVPIEEL FAAGGIAEKN PFVMQIYADV TNMDIKISGS PQAPALGSAI 490 500 510 520 530 540 FGALAAGKEK GGYDDIKKAA ANMGKLKDIT YTPNAENAAV YEKLYAEYKE LVHYFGKENH 550 560 VMKRLKTIKN LQFSSAAKKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression." Sa-Nogueira I.M.G., Nogueira T.V., Soares S., de Lencastre H. Microbiology 143:957-969(1997) [PubMed: 9084180] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism." Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R. Microbiology 142:3067-3078(1996) [PubMed: 8969504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis." Mota L.J., Tavares P., Sa-Nogueira I.M.G. Mol. Microbiol. 33:476-489(1999) [PubMed: 10417639] [Abstract] Cited for: TRANSCRIPTIONAL REGULATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89408 Genomic DNA. Translation: CAA61586.1. Z75208 Genomic DNA. Translation: CAA99588.1. AL009126 Genomic DNA. Translation: CAB14839.1.
PIR	D69587.
RefSeq	NP_390757.1.
3D structure databases
SMR	P94524. Positions 2-532.
ModBase	Search...
Genome annotation databases
GeneID	936019.
GenomeReviews	Gene locus BSU28790 in contig AL009126_GR.
KEGG	bsu:BSU28790.
NMPDR	fig|224308.1.peg.2882.
Organism-specific databases
SubtiList	BG11905. araB. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG312681.
OMA	EATAYGT.
PhylomeDB	P94524.
Enzyme and pathway databases
BRENDA	2.7.1.16. 150.
Family and domain databases
HAMAP	MF_00520. Ribulokinase. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005929. L_ribulokin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01234. L-ribulokinase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARAB_BACSU

Accession

Primary (citable) accession number: P94524
Secondary accession number(s): O05185

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	February 9, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)