Skip Header

Contribute Send feedback
Read comments (?) or add your own

P94523 (ARAA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
L-arabinose isomerase
EC=5.3.1.4
Gene names
Name:araA
Ordered Locus Names:BSU28800
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the conversion of L-arabinose to L-ribulose By similarity. HAMAP MF_00519

Catalytic activity

L-arabinose = L-ribulose. HAMAP MF_00519

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP MF_00519

Pathway

Carbohydrate degradation; L-arabinose degradation via L-ribulose; D-xylulose 5-phosphate from L-arabinose (bacterial route): step 1/3. HAMAP MF_00519

Induction

Transcription is repressed by glucose and by the binding of AraR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of AraR to the DNA, thus allowing expression of the gene. Ref.5

Sequence similarities

Belongs to the arabinose isomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496L-arabinose isomerase HAMAP MF_00519
PRO_0000198382

Sites

Metal binding3051Manganese By similarity
Metal binding3301Manganese By similarity
Metal binding3471Manganese By similarity
Metal binding4461Manganese By similarity

Experimental info

Sequence conflict85 – 9410AKMWIEGLSS → SQKLWKRRPFPP in CAA99587. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P94523 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: E0DCF8B736364F18

FASTA49656,122
        10         20         30         40         50         60 
MLQTKDYEFW FVTGSQHLYG EETLELVDQH AKSICEGLSG ISSRYKITHK PVVTSPETIR 

        70         80         90        100        110        120 
ELLREAEYSE TCAGIITWMH TFSPAKMWIE GLSSYQKPLM HLHTQYNRDI PWGTIDMDFM 

       130        140        150        160        170        180 
NSNQSAHGDR EYGYINSRMG LSRKVIAGYW DDEEVKKEMS QWMDTAAALN ESRHIKVARF 

       190        200        210        220        230        240 
GDNMRHVAVT DGDKVGAHIQ FGWQVDGYGI GDLVEVMDRI TDDEVDTLYA EYDRLYVISE 

       250        260        270        280        290        300 
ETKRDEAKVA SIKEQAKIEL GLTAFLEQGG YTAFTTSFEV LHGMKQLPGL AVQRLMEKGY 

       310        320        330        340        350        360 
GFAGEGDWKT AALVRMMKIM AKGKRTSFME DYTYHFEPGN EMILGSHMLE VCPTVALDQP 

       370        380        390        400        410        420 
KIEVHSLSIG GKEDPARLVF NGISGSAIQA SIVDIGGRFR LVLNEVNGQE IEKDMPNLPV 

       430        440        450        460        470        480 
ARVLWKPEPS LKTAAEAWIL AGGAHHTCLS YELTAEQMLD WAEMAGIESV LISRDTTIHK 

       490 
LKHELKWNEA LYRLQK

« Hide

References

« Hide 'large scale' references
[1]"The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression."
Sa-Nogueira I.M.G., Nogueira T.V., Soares S., de Lencastre H.
Microbiology 143:957-969(1997) [PubMed: 9084180] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism."
Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R.
Microbiology 142:3067-3078(1996) [PubMed: 8969504] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 85-94.
[5]"Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis."
Mota L.J., Tavares P., Sa-Nogueira I.M.G.
Mol. Microbiol. 33:476-489(1999) [PubMed: 10417639] [Abstract]
Cited for: TRANSCRIPTIONAL REGULATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X89408 Genomic DNA. Translation: CAA61585.1.
Z75208 Genomic DNA. Translation: CAA99587.1.
AL009126 Genomic DNA. Translation: CAB14840.2.
PIRC69587.
RefSeqNP_390758.2. NC_000964.3.

3D structure databases

ProteinModelPortalP94523.
SMRP94523. Positions 1-494.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000004100; EBBACP00000004100; EBBACG00000004092.
GeneID936764.
GenomeReviewsGene locus BSU28800 in contig AL009126_GR.
KEGGbsu:BSU28800.
PATRIC18977652. VBIBacSub10457_3014.

Organism-specific databases

GenoListBSU28800. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000001793.
HOGENOMHBG297198.
PhylomeDBP94523.
ProtClustDBPRK02929.

Enzyme and pathway databases

BioCycBSUB:BSU28800-MONOMER.
BRENDA5.3.1.4. 700.

Family and domain databases

HAMAPMF_00519. Arabinose_Isome.
[Tree]
InterProIPR024664. Ara_Isoase_C.
IPR004216. Fuc/Ara_isomerase_C.
IPR009015. Fucose_isomerase_N/cen.
IPR003762. Lara_isomerase.
[Graphical view]
KOK01804.
PfamPF11762. Arabinose_Iso_C. 1 hit.
PF02610. Arabinose_Isome. 1 hit.
[Graphical view]
PIRSFPIRSF001478. L-ara_isomerase. 1 hit.
ProDomPD018364. Lara_isomerase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF50443. Fuc_isomerase_C. 1 hit.
SSF53743. Fuc_isomerase_N. 1 hit.
ProtoNetSearch...

Entry information

Entry nameARAA_BACSU
AccessionPrimary (citable) accession number: P94523
Secondary accession number(s): O05184
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2002
Last modified: January 25, 2012
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents