P94494 (ALR2_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase 2
EC=5.1.1.1

Gene names

Name:	alr2
Synonyms:	yncD
Ordered Locus Names:	BSU17640

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Alanine racemase 2 HAMAP MF_01201		PRO_0000114501
Sites
	Active site	39	1	Proton acceptor; specific for D-alanine By similarity
	Active site	272	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	39	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P94494 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: FE446C21107FBA6E	FASTA	394	43,648
	10 20 30 40 50 60 MIKLCREVWI EVNLDAVKKN LRAIRRHIPH KSKIMAVVKA NGYGHGSIEV ARHALEHGAS 70 80 90 100 110 120 ELAVASVEEG IVLRKAGITA PILVLGFTSL SCVKKSAAWN ITLSAFQVDW MKEANEILEK 130 140 150 160 170 180 EASANRLAIH INVDTGMGRL GVRTKEELLE VVKALKASKF LRWTGIFTHF STADEPDTTL 190 200 210 220 230 240 TKLQHEKFIS FLSFLKKQGI ELPTVHMCNT AAAIAFPEFS ADMIRLGIGL YGLYPSAYIK 250 260 270 280 290 300 QLNLVKLEPA LSLKARIAYV KTMRTEPRTV SYGATYIAEP NEVIATLPIG YADGYSRALS 310 320 330 340 350 360 NRGFVLHRGK RVPVAGRVTM DMIMVSLGEN GEGKQGDEVV IYGKQKGAEI SVDEVAEMLN 370 380 390 TINYEVVSTL SRRIPRFYIR DGEIFKVSTP VLYV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 26 kb region of the Bacillus subtilis genome downstream of spoVJ." Borchert S., Klein C., Piksa B., Hammelmann M., Entian K.-D. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U66480 Genomic DNA. Translation: AAB41097.1. AL009126 Genomic DNA. Translation: CAB13648.1.
PIR	F69888.
RefSeq	NP_389646.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P94494.
SMR	P94494. Positions 3-391.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000002352; EBBACP00000002352; EBBACG00000002347.
GeneID	939550.
GenomeReviews	Gene locus BSU17640 in contig AL009126_GR.
KEGG	bsu:BSU17640.
NMPDR	fig|224308.1.peg.1768.
PATRIC	18975345. VBIBacSub10457_1864.
Organism-specific databases
GenoList	BSU17640. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000001615.
HOGENOM	HBG712172.
OMA	MLNTINY.
PhylomeDB	P94494.
ProtClustDB	CLSK887341.
Enzyme and pathway databases
BioCyc	BSUB:BSU17640-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR2_BACSU

Accession

Primary (citable) accession number: P94494

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	January 25, 2012
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program