Reviewed, UniProtKB/Swiss-Prot P94494 (ALR2_BACSU)

Names and origin

Protein names

Recommended name:
    Alanine racemase 2
    EC=5.1.1.1

Gene names

Name:	alr2
Synonyms:	yncD
Ordered Locus Names:	BSU17640

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Alanine racemase 2 HAMAP MF_01201		PRO_0000114501
Sites
	Active site	39	1	Proton acceptor; specific for D-alanine By similarity
	Active site	272	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	39	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P94494-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: FE446C21107FBA6E	FASTA	394	43,648
	10 20 30 40 50 60 MIKLCREVWI EVNLDAVKKN LRAIRRHIPH KSKIMAVVKA NGYGHGSIEV ARHALEHGAS 70 80 90 100 110 120 ELAVASVEEG IVLRKAGITA PILVLGFTSL SCVKKSAAWN ITLSAFQVDW MKEANEILEK 130 140 150 160 170 180 EASANRLAIH INVDTGMGRL GVRTKEELLE VVKALKASKF LRWTGIFTHF STADEPDTTL 190 200 210 220 230 240 TKLQHEKFIS FLSFLKKQGI ELPTVHMCNT AAAIAFPEFS ADMIRLGIGL YGLYPSAYIK 250 260 270 280 290 300 QLNLVKLEPA LSLKARIAYV KTMRTEPRTV SYGATYIAEP NEVIATLPIG YADGYSRALS 310 320 330 340 350 360 NRGFVLHRGK RVPVAGRVTM DMIMVSLGEN GEGKQGDEVV IYGKQKGAEI SVDEVAEMLN 370 380 390 TINYEVVSTL SRRIPRFYIR DGEIFKVSTP VLYV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 26 kb region of the Bacillus subtilis genome downstream of spoVJ." Borchert S., Klein C., Piksa B., Hammelmann M., Entian K.-D. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U66480 Genomic DNA. Translation: AAB41097.1. AL009126 Genomic DNA. Translation: CAB13648.1.
PIR	F69888.
RefSeq	NP_389646.1.
3D structure databases
HSSP	HSSP built from PDB template 1BD0 based on UniProtKB P10724.
ModBase	Search...
Genome annotation databases
GeneID	939550.
GenomeReviews	Gene locus BSU17640 in contig AL009126_GR.
KEGG	bsu:BSU17640.
NMPDR	fig|224308.1.peg.1768.
Organism-specific databases
SubtiList	BG12267. alr2. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P94494.
OMA	KSKIMAV.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU1765-MON.
BRENDA	5.1.1.1. 150.
Family and domain databases
HAMAP	MF_01201. [Tree]
InterPro	IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR000821. Ala_racemase_reg. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR2_BACSU

Accession

Primary (citable) accession number: P94494

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	November 3, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)