Reviewed, UniProtKB/Swiss-Prot P94469 (LEVB_BACST)

Names and origin

Protein names

Recommended name:
    Levanbiose-producing levanase
    EC=3.2.1.64
Alternative name(s):
    2,6-beta-fructan 6-levanbiohydrolase
    Levanase

Gene names

Name:	levB
Synonyms:	surC, surD

Organism

Bacillus stearothermophilus (Geobacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	395 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the degradation of levan mainly into levanbiose (difructose) By similarity. Can also hydrolyze inulin. Ref.1
Catalytic activity	Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i.e. 6-(beta-D-fructofuranosyl)-D-fructose, from the end of the chain.
Subcellular location	Membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.
Biophysicochemical properties	Temperature dependence: Optimum temperature is 55 degrees Celsius.
Sequence caution	The sequence AAB97112.1 differs from that shown. Reason: Frameshift at positions 40 and 351.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2,6-beta-fructan 6-levanbiohydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›395	›395	Levanbiose-producing levanase		PRO_0000344253
Regions
	Region	59 – 60	2	Substrate binding By similarity
	Region	124 – 125	2	Substrate binding By similarity
Sites
	Active site	1	1	By similarity
	Binding site	173	1	Substrate By similarity
	Binding site	261	1	Substrate By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	395	1

Sequences

	Sequence		Length	Mass (Da)
	P94469-1 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 1ECDBAFE754F9DE3	FASTA	395	45,098
	10 20 30 40 50 60 DPQKPIYFNG KYHYYYLYNR GYPKGNGTEW RHYVSDDLVH WTDEGVAIPK YTNPDGDIWT 70 80 90 100 110 120 GSVVVDKENT AGFGKNALVA IVTQPSAKDK KQEQYLWYST DKGKSFKFYS GNPVMPNPGT 130 140 150 160 170 180 DDFRDPKVIW DDQDNKWVMV MAEGSKIGFY ESDNLKDWHY TSGFFPEQTG MVECPDLYMM 190 200 210 220 230 240 RASDGTNKWV LGASANGKPW GKPNTYAYWT GSFDGKEFKA DQTEAQWLDY GFDWYGGVTF 250 260 270 280 290 300 EDSKSTDPLE KRYALAWMNN WDYANNTPTM KNGFNGTDSV IREIRLKEQD GTYSLVSQPI 310 320 330 340 350 360 EALEQLTVST DEIEDQDVNG SKTLSITGDT YQLDTDLSWS ELKNAGVRLR ESEDQKRHID 370 380 390 VGIFAEDGYA YVNRAATNQP DKSNTYVESK APYDV

References

[1]	"A novel levansucrase-levanase gene cluster in Bacillus stearothermophilus ATCC12980." Li Y., Triccas J.A., Ferenci T. Biochim. Biophys. Acta 1353:203-208(1997) [PubMed: 9349714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TEMPERATURE DEPENDENCE. Strain: ATCC 12980 / DSM 22 / IFO 12550 / NCIB 8923 / NCTC 10339.
[2]	"Homologous locus of Bacillus subtilis and Bacillus stearothermophilus genomes containing levansucrase and levanase genes." Naumoff D.G. Mol. Biol. (Mosk.) 33:207-210(1999) [PubMed: 10377564] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFTS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U34875 Genomic DNA. Translation: AAB97112.1. Frameshift.
3D structure databases
SMR	P94469. Positions 1-384.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Family and domain databases
InterPro	IPR001362. Glyco_hydro_32. IPR013148. Glyco_hydro_32_N. [Graphical view]
Pfam	PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEVB_BACST

Accession

Primary (citable) accession number: P94469

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	January 19, 2010
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)