P94468 (SACB_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inactive levansucrase

Gene names

Name:	sacB
Synonyms:	surB

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 68 family.
Caution	According to Ref.1, does not have levansucrase activity, despite a very strong similarity to sacB from B.subtilis. Ser-356 and Gln-363 are present instead of the conserved Phe and positively charged residue Lys/His/Arg, respectively, which could be important for activity. These point mutations might explain the inactivity of the enzyme.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Gene Ontology (GO)
Biological process	carbohydrate utilization Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	levansucrase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	By similarity
	Chain	30 – 473	444	Inactive levansucrase		PRO_0000012248

Sequences

	Sequence		Length	Mass (Da)
	P94468 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 12F7A272EC2B771E	FASTA	473	52,855
	10 20 30 40 50 60 MNIKKFAKQA TVLTFTTALL AGGATQAFAK ETNQKPYKET YGISHITRHD MLQIPEQQKN 70 80 90 100 110 120 EKYQVPEFDS STIKNISSAK GLDVWDSWPL QNADGTVANY HGYHIVFALA GDPKNADDTS 130 140 150 160 170 180 IYMFYQKVGE TSIDSWKTPG RVFKDSDKFD ANDSILKDQT QEWSGSATFT SDGKIRLFYT 190 200 210 220 230 240 DFSGKHYGKQ TLTTAQVNVS ASDSSLNING VEDYKSIFDG DSKTYQNVQQ FIDEGNYSSG 250 260 270 280 290 300 DNHTLRDPHY VEDKGHKYLV FEANTGTEDG YQGEESLFNK AYYGKSTSFF RQESQKLLQS 310 320 330 340 350 360 DKNRTAELAN GALGMIELND DYTLKKVMKP LIASNTVTDE IERANVFKMN GKWYLSTDSR 370 380 390 400 410 420 GSQMTIDGIT SNDIYMLGYV SNSLTGPYKP LNKTGLVLKM DLDPNDVTFT YSHFAVPQAT 430 440 450 460 470 GNNVVITSYM TNRGFYADKQ STFAPSFLLN IQGKKTSVVK ASILDQGQLT VNQ

References

[1]	"A novel levansucrase-levanase gene cluster in Bacillus stearothermophilus ATCC12980." Li Y., Triccas J.A., Ferenci T. Biochim. Biophys. Acta 1353:203-208(1997) [PubMed: 9349714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], LACK OF FUNCTION AS A LEVANSUCRASE. Strain: ATCC 12980 / DSM 22 / JCM 2501 / NBRC 12550 / NCIMB 8923 / NCTC 10339 / VKM B-510.
[2]	"Homologous locus of Bacillus subtilis and Bacillus stearothermophilus genomes containing levansucrase and levanase genes." Naumoff D.G. Mol. Biol. (Mosk.) 33:207-210(1999) [PubMed: 10377564] [Abstract] Cited for: DISCUSSION OF SEQUENCE WITH RESPECT TO INACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U34874 Genomic DNA. Translation: AAB97111.1.
3D structure databases
ProteinModelPortal	P94468.
SMR	P94468. Positions 34-473.
ModBase	Search...
Protein family/group databases
CAZy	GH68. Glycoside Hydrolase Family 68.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR023296. Glyco_hydro_43_beta-prop. IPR003469. Glyco_hydro_68. [Graphical view]
Gene3D	G3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 2 hits.
Pfam	PF02435. Glyco_hydro_68. 1 hit. [Graphical view]
SUPFAM	SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SACB_GEOSE

Accession

Primary (citable) accession number: P94468

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	December 14, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program