Reviewed,
UniProtKB/Swiss-Prot P94468 (SACB_BACST)
Last modified
June 16, 2009.
Version 45.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Inactive levansucrase | ||||
| Gene names |
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| Organism | Bacillus stearothermophilus (Geobacillus stearothermophilus) | ||||
| Taxonomic identifier | 1422 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus |
Protein attributes
| Sequence length | 473 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Subcellular location | Secreted Potential. |
| Sequence similarities | Belongs to the glycosyl hydrolase 68 family. |
| Caution | According to Ref.1, does not have levansucrase activity, despite a very strong similarity to sacB from B.subtilis. Ser-356 and Gln-363 are present instead of the conserved Phe and positively charged residue Lys/His/Arg, respectively, which could be important for activity. These point mutations might explain the inactivity of the enzyme. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Secreted |
| Domain | Signal |
| Gene Ontology (GO) | |
| Biological process | sugar utilization Inferred from electronic annotation. Source: InterPro |
| Cellular component | extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | levansucrase activity Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| [1] | "A novel levansucrase-levanase gene cluster in Bacillus stearothermophilus ATCC12980." Li Y., Triccas J.A., Ferenci T. Biochim. Biophys. Acta 1353:203-208(1997) [PubMed: 9349714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], LACK OF FUNCTION AS A LEVANSUCRASE. Strain: ATCC 12980 / DSM 22 / IFO 12550 / NCIB 8923 / NCTC 10339. |
| [2] | "Homologous locus of Bacillus subtilis and Bacillus stearothermophilus genomes containing levansucrase and levanase genes." Naumoff D.G. Mol. Biol. (Mosk.) 33:207-210(1999) [PubMed: 10377564] [Abstract] Cited for: DISCUSSION OF SEQUENCE WITH RESPECT TO INACTIVITY. |
Cross-references
Sequence databases | |
|---|---|
| U34874 Genomic DNA. Translation: AAB97111.1. | |
3D structure databases | |
| SMR | P94468. Positions 34-473. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GH68. Glycoside Hydrolase Family 68. |
Enzyme and pathway databases | |
| BRENDA | 2.4.1.10. 266715. |
Family and domain databases | |
| InterPro | IPR003469. Glyco_hydro_68. [Graphical view] |
| Pfam | PF02435. Glyco_hydro_68. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | SACB_BACST | ||||||||
| Accession | Primary (citable) accession number: P94468 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


